GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT
This research was carried out to study genetic diversity of ampicillin-resistant Vibrio from various stages of tiger shrimp larvae (Penaeus Monodon) development from,Tambak Inti Rakyat hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates w...
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2011-11-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/160 |
| _version_ | 1797758582041608192 |
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| author | WIDANARNI WIDANARNI ANTONIUS SUWANTO |
| author_facet | WIDANARNI WIDANARNI ANTONIUS SUWANTO |
| author_sort | WIDANARNI WIDANARNI |
| collection | DOAJ |
| description | This research was carried out to study genetic diversity of ampicillin-resistant Vibrio from
various stages of tiger shrimp larvae (Penaeus Monodon) development from,Tambak Inti Rakyat
hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were
isolated using thiosulphate citrate bile-salt sucrose agar (TCBS-Agar) and seawater complete
agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be
grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae
and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also
present in their respective rearing water of each stage and some natural feed. Schizotyping
analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates
could be grouped into at least 13 different genotypes. Therefore, schizotyping was more
discriminative than physiological characterization. This study showed that particular groups of Vibrio
colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups
of Vibrio might be the dominant microbiota which could suppress the development of other Vibrio
including the pathogenic Vibrio.
Key words : Shrimp/ampicillin-resistant K/fcno/schizotyping |
| first_indexed | 2024-03-12T18:32:04Z |
| format | Article |
| id | doaj.art-90769ee8512f4926aa62795e94058fef |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-12T18:32:04Z |
| publishDate | 2011-11-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-90769ee8512f4926aa62795e94058fef2023-08-02T08:16:27ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2011-11-011510.11598/btb.2000.0.15.160GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENTWIDANARNI WIDANARNI0ANTONIUS SUWANTO1"Department of Aquaculture, Faculty of Fisheries and Marine Science Bogor Agricultural UniversityDepartment of Biology, Faculty of Science and Mathematics, and IUC Biotechnology, Bogor Agricultural University, Bogor 16680, Indonesia 'SEAMEO-BIOTROP, JI. Raya Tajur, Km 6 Bogor, IndonesiaThis research was carried out to study genetic diversity of ampicillin-resistant Vibrio from various stages of tiger shrimp larvae (Penaeus Monodon) development from,Tambak Inti Rakyat hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were isolated using thiosulphate citrate bile-salt sucrose agar (TCBS-Agar) and seawater complete agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also present in their respective rearing water of each stage and some natural feed. Schizotyping analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates could be grouped into at least 13 different genotypes. Therefore, schizotyping was more discriminative than physiological characterization. This study showed that particular groups of Vibrio colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups of Vibrio might be the dominant microbiota which could suppress the development of other Vibrio including the pathogenic Vibrio. Key words : Shrimp/ampicillin-resistant K/fcno/schizotypinghttps://journal.biotrop.org/index.php/biotropia/article/view/160 |
| spellingShingle | WIDANARNI WIDANARNI ANTONIUS SUWANTO GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT |
| title_full | GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT |
| title_fullStr | GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT |
| title_full_unstemmed | GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT |
| title_short | GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT |
| title_sort | genetic diversity of ampicillin resistant vibrio isolated from various stages of tiger shrimp larvae development |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/160 |
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