In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid
Fowl typhoid, a septicaemic disease of poultry, is caused by <i>Salmonella</i> Gallinarum and leads to severe economic losses. The aim of the present study was to isolate, select and characterize indigenous probiotic lactobacilli with anti-<i>Salmonella</i> Gallinarum activit...
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MDPI AG
2023-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/8/1284 |
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| author | Adnan Mehmood Muhammad Nawaz Masood Rabbani Muhammad Hassan Mushtaq |
| author_facet | Adnan Mehmood Muhammad Nawaz Masood Rabbani Muhammad Hassan Mushtaq |
| author_sort | Adnan Mehmood |
| collection | DOAJ |
| description | Fowl typhoid, a septicaemic disease of poultry, is caused by <i>Salmonella</i> Gallinarum and leads to severe economic losses. The aim of the present study was to isolate, select and characterize indigenous probiotic lactobacilli with anti-<i>Salmonella</i> Gallinarum activity. A total 55 lactobacilli were isolated from the caeca and ileum parts of healthy chickens and identified to species level by 16S rDNA sequencing. All the isolates were initially screened for antimicrobial activity and selected isolates were further subjected to in vitro evaluation of probiotic properties. Lactobacilli isolates (<i>n</i> = 21) showed varying levels of activity (08–18 mm) against <i>Salmonella</i> Gallinarum. These selected isolates also showed tolerance to acidic conditions (pH 3 and 4). Out of these 21 isolates, 13 showed growth (>0.5 OD at 600 nm) 0.3% bile salts. Moreover, these isolates also had the ability of auto-aggregation (20.05 ± 0.62%–50.70 ± 1.40%), and co-aggregation with <i>Salmonella</i> Gallinarum (5.22 ± 0.21%–42.07 ± 0.70%). Results revealed that lactobacilli had a higher level of resistance to vancomycin (100%), streptomycin (100%), ciprofloxacin (95%), gentamicin (90%), doxycycline (90%), oxytetracycline (85%), and bacitracin (80%), and a lower level of resistance to penicillin (33%), erythromycin (28%), chloramphenicol (23%), fusidic acid (23%) and amoxicillin (4%). The <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 were sensitive to most of the antibiotics. The overall results revealed that two <i>Limosilactobacillus fermentum</i> strains (PC-10 and PC-76) fulfill the in vitro selection criteria of probiotics, i.e, tolerance to low pH, resistance to bile salts, auto-aggregation, co-aggregation with <i>Salmonella</i> Gallinarum, and absence of acquired antibiotic resistance. The <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 also inhibited the (>5 log10) growth of <i>Salmonella</i> Gallinarum in co-culture assay. It is concluded that <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 may be further investigated and developed as anti-<i>Salmonella</i> Gallinarum probiotics for poultry. |
| first_indexed | 2024-03-11T05:19:06Z |
| format | Article |
| id | doaj.art-90789575f6af422787f5795503d67f87 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:19:06Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-90789575f6af422787f5795503d67f872023-11-17T17:59:34ZengMDPI AGAnimals2076-26152023-04-01138128410.3390/ani13081284In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl TyphoidAdnan Mehmood0Muhammad Nawaz1Masood Rabbani2Muhammad Hassan Mushtaq3Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore 54000, PakistanFowl typhoid, a septicaemic disease of poultry, is caused by <i>Salmonella</i> Gallinarum and leads to severe economic losses. The aim of the present study was to isolate, select and characterize indigenous probiotic lactobacilli with anti-<i>Salmonella</i> Gallinarum activity. A total 55 lactobacilli were isolated from the caeca and ileum parts of healthy chickens and identified to species level by 16S rDNA sequencing. All the isolates were initially screened for antimicrobial activity and selected isolates were further subjected to in vitro evaluation of probiotic properties. Lactobacilli isolates (<i>n</i> = 21) showed varying levels of activity (08–18 mm) against <i>Salmonella</i> Gallinarum. These selected isolates also showed tolerance to acidic conditions (pH 3 and 4). Out of these 21 isolates, 13 showed growth (>0.5 OD at 600 nm) 0.3% bile salts. Moreover, these isolates also had the ability of auto-aggregation (20.05 ± 0.62%–50.70 ± 1.40%), and co-aggregation with <i>Salmonella</i> Gallinarum (5.22 ± 0.21%–42.07 ± 0.70%). Results revealed that lactobacilli had a higher level of resistance to vancomycin (100%), streptomycin (100%), ciprofloxacin (95%), gentamicin (90%), doxycycline (90%), oxytetracycline (85%), and bacitracin (80%), and a lower level of resistance to penicillin (33%), erythromycin (28%), chloramphenicol (23%), fusidic acid (23%) and amoxicillin (4%). The <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 were sensitive to most of the antibiotics. The overall results revealed that two <i>Limosilactobacillus fermentum</i> strains (PC-10 and PC-76) fulfill the in vitro selection criteria of probiotics, i.e, tolerance to low pH, resistance to bile salts, auto-aggregation, co-aggregation with <i>Salmonella</i> Gallinarum, and absence of acquired antibiotic resistance. The <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 also inhibited the (>5 log10) growth of <i>Salmonella</i> Gallinarum in co-culture assay. It is concluded that <i>Limosilactobacillus fermentum</i> PC-10 and PC-76 may be further investigated and developed as anti-<i>Salmonella</i> Gallinarum probiotics for poultry.https://www.mdpi.com/2076-2615/13/8/1284fowl typhoid<i>Salmonella</i> Gallinarumprobiotics<i>Limosilactobacillus fermentum</i> |
| spellingShingle | Adnan Mehmood Muhammad Nawaz Masood Rabbani Muhammad Hassan Mushtaq In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid Animals fowl typhoid <i>Salmonella</i> Gallinarum probiotics <i>Limosilactobacillus fermentum</i> |
| title | In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid |
| title_full | In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid |
| title_fullStr | In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid |
| title_full_unstemmed | In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid |
| title_short | In Vitro Characterization of Probiotic Potential of <i>Limosilactobacillus fermentum</i> against <i>Salmonella</i> Gallinarum Causing Fowl Typhoid |
| title_sort | in vitro characterization of probiotic potential of i limosilactobacillus fermentum i against i salmonella i gallinarum causing fowl typhoid |
| topic | fowl typhoid <i>Salmonella</i> Gallinarum probiotics <i>Limosilactobacillus fermentum</i> |
| url | https://www.mdpi.com/2076-2615/13/8/1284 |
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