Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters
The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fer...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-12-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2014;volume=16;issue=6;spage=838;epage=844;aulast=Sarrate |
| _version_ | 1811233107659980800 |
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| author | Zaida Sarrate Francesca Vidal Joan Blanco |
| author_facet | Zaida Sarrate Francesca Vidal Joan Blanco |
| author_sort | Zaida Sarrate |
| collection | DOAJ |
| description | The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants. |
| first_indexed | 2024-04-12T11:14:50Z |
| format | Article |
| id | doaj.art-907b4a0c117e427789a0e1a535b55442 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-04-12T11:14:50Z |
| publishDate | 2014-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-907b4a0c117e427789a0e1a535b554422022-12-22T03:35:31ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622014-12-0116683884410.4103/1008-682X.135126Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parametersZaida SarrateFrancesca VidalJoan BlancoThe aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2014;volume=16;issue=6;spage=838;epage=844;aulast=Sarratechiasmata count; human spermatocytes; multiplex fluorescence in situ hybridization; polymorphic karyotype; seminal parameters |
| spellingShingle | Zaida Sarrate Francesca Vidal Joan Blanco Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters Asian Journal of Andrology chiasmata count; human spermatocytes; multiplex fluorescence in situ hybridization; polymorphic karyotype; seminal parameters |
| title | Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters |
| title_full | Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters |
| title_fullStr | Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters |
| title_full_unstemmed | Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters |
| title_short | Meiotic abnormalities in metaphase I human spermatocytes from infertile males: frequencies, chromosomes involved, and the relationships with polymorphic karyotype and seminal parameters |
| title_sort | meiotic abnormalities in metaphase i human spermatocytes from infertile males frequencies chromosomes involved and the relationships with polymorphic karyotype and seminal parameters |
| topic | chiasmata count; human spermatocytes; multiplex fluorescence in situ hybridization; polymorphic karyotype; seminal parameters |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2014;volume=16;issue=6;spage=838;epage=844;aulast=Sarrate |
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