Circadian regulation of the Drosophila astrocyte transcriptome.
Recent studies have demonstrated that astrocytes cooperate with neurons of the brain to mediate circadian control of many rhythmic processes including locomotor activity and sleep. Transcriptional profiling studies have described the overall rhythmic landscape of the brain, but few have employed app...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-09-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009790 |
| _version_ | 1811188955585970176 |
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| author | Samantha You Alder M Yu Mary A Roberts Ivanna J Joseph F Rob Jackson |
| author_facet | Samantha You Alder M Yu Mary A Roberts Ivanna J Joseph F Rob Jackson |
| author_sort | Samantha You |
| collection | DOAJ |
| description | Recent studies have demonstrated that astrocytes cooperate with neurons of the brain to mediate circadian control of many rhythmic processes including locomotor activity and sleep. Transcriptional profiling studies have described the overall rhythmic landscape of the brain, but few have employed approaches that reveal heterogeneous, cell-type specific rhythms of the brain. Using cell-specific isolation of ribosome-bound RNAs in Drosophila, we constructed the first circadian "translatome" for astrocytes. This analysis identified 293 "cycling genes" in astrocytes, most with mammalian orthologs. A subsequent behavioral genetic screen identified a number of genes whose expression is required in astrocytes for normal sleep behavior. In particular, we show that certain genes known to regulate fly innate immune responses are also required for normal sleep patterns. |
| first_indexed | 2024-04-11T14:26:38Z |
| format | Article |
| id | doaj.art-907c60a3d5454f08ab1c50de388699c3 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-11T14:26:38Z |
| publishDate | 2021-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-907c60a3d5454f08ab1c50de388699c32022-12-22T04:18:50ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-09-01179e100979010.1371/journal.pgen.1009790Circadian regulation of the Drosophila astrocyte transcriptome.Samantha YouAlder M YuMary A RobertsIvanna J JosephF Rob JacksonRecent studies have demonstrated that astrocytes cooperate with neurons of the brain to mediate circadian control of many rhythmic processes including locomotor activity and sleep. Transcriptional profiling studies have described the overall rhythmic landscape of the brain, but few have employed approaches that reveal heterogeneous, cell-type specific rhythms of the brain. Using cell-specific isolation of ribosome-bound RNAs in Drosophila, we constructed the first circadian "translatome" for astrocytes. This analysis identified 293 "cycling genes" in astrocytes, most with mammalian orthologs. A subsequent behavioral genetic screen identified a number of genes whose expression is required in astrocytes for normal sleep behavior. In particular, we show that certain genes known to regulate fly innate immune responses are also required for normal sleep patterns.https://doi.org/10.1371/journal.pgen.1009790 |
| spellingShingle | Samantha You Alder M Yu Mary A Roberts Ivanna J Joseph F Rob Jackson Circadian regulation of the Drosophila astrocyte transcriptome. PLoS Genetics |
| title | Circadian regulation of the Drosophila astrocyte transcriptome. |
| title_full | Circadian regulation of the Drosophila astrocyte transcriptome. |
| title_fullStr | Circadian regulation of the Drosophila astrocyte transcriptome. |
| title_full_unstemmed | Circadian regulation of the Drosophila astrocyte transcriptome. |
| title_short | Circadian regulation of the Drosophila astrocyte transcriptome. |
| title_sort | circadian regulation of the drosophila astrocyte transcriptome |
| url | https://doi.org/10.1371/journal.pgen.1009790 |
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