Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation
Background : The contributions of the gut microbiota to obesity and metabolic disease represent a potentially modifiable factor that may explain variation in risk between individuals. This study aimed to explore relationships among microbial composition and imputed functional attributes, a range of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society for the Study of Obesity
2024-03-01
|
| Series: | Journal of Obesity & Metabolic Syndrome |
| Subjects: | |
| Online Access: | http://www.jomes.org/journal/view.html?doi=10.7570/jomes23022 |
| _version_ | 1797235343894773760 |
|---|---|
| author | Kyle M. Hatton-Jones Nicholas P. West Mike W.C. Thang Pin-Yen Chen Peter Davoren Allan W. Cripps Amanda J. Cox |
| author_facet | Kyle M. Hatton-Jones Nicholas P. West Mike W.C. Thang Pin-Yen Chen Peter Davoren Allan W. Cripps Amanda J. Cox |
| author_sort | Kyle M. Hatton-Jones |
| collection | DOAJ |
| description | Background : The contributions of the gut microbiota to obesity and metabolic disease represent a potentially modifiable factor that may explain variation in risk between individuals. This study aimed to explore relationships among microbial composition and imputed functional attributes, a range of soluble metabolic and immune indices, and gene expression markers in males with or without evidence of metabolic dysregulation (MetDys). Methods : This case-control study included healthy males (n=15; 41.9±11.7 years; body mass index [BMI], 22.9±1.2 kg/m2) and males with evidence of MetDys (n=14; 46.6±10.0 years; BMI, 35.1±3.3 kg/m2) who provided blood and faecal samples for assessment of a range of metabolic and immune markers and microbial composition using 16S rRNA gene sequencing. Metagenomic functions were imputed from microbial sequence data for analysis. Results : In addition to elevated values in a range of traditional metabolic, adipokine and inflammatory indices in the MetDys group, 23 immunomodulatory genes were significantly altered in the MetDys group. Overall microbial diversity did not differ between groups; however, a trend for a higher relative abundance of the Bacteroidetes (P=0.06) and a lower relative abundance of the Verrucomicrobia (P=0.09) phyla was noted in the MetDys group. Using both family- and genera-level classifications, a partial least square discriminant analysis revealed unique microbial signatures between the groups. Conclusion : These findings confirm the need for ongoing investigations in human clinical cohorts to further resolve the relationships between the gut microbiota and metabolic and immune markers and risk for metabolic disease. |
| first_indexed | 2024-04-24T16:46:27Z |
| format | Article |
| id | doaj.art-907c6fde88ad4c48a8ea515c0b523831 |
| institution | Directory Open Access Journal |
| issn | 2508-6235 |
| language | English |
| last_indexed | 2024-04-24T16:46:27Z |
| publishDate | 2024-03-01 |
| publisher | Korean Society for the Study of Obesity |
| record_format | Article |
| series | Journal of Obesity & Metabolic Syndrome |
| spelling | doaj.art-907c6fde88ad4c48a8ea515c0b5238312024-03-29T07:47:10ZengKorean Society for the Study of ObesityJournal of Obesity & Metabolic Syndrome2508-62352024-03-01331647510.7570/jomes23022jomes23022Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic DysregulationKyle M. Hatton-Jones0Nicholas P. West1Mike W.C. Thang2Pin-Yen Chen3Peter Davoren4Allan W. Cripps5Amanda J. Cox6School of Pharmacy and Medical Science, Griffith University, Southport, AustraliaSchool of Pharmacy and Medical Science, Griffith University, Southport, AustraliaQCIF Facility for Advanced Bioinformatics, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, AustraliaSchool of Pharmacy and Medical Science, Griffith University, Southport, AustraliaDiabetes and Endocrinology, Gold Coast University Hospital, Southport, AustraliaMenzies Health Institute Queensland, Griffith University, Southport, AustraliaSchool of Pharmacy and Medical Science, Griffith University, Southport, AustraliaBackground : The contributions of the gut microbiota to obesity and metabolic disease represent a potentially modifiable factor that may explain variation in risk between individuals. This study aimed to explore relationships among microbial composition and imputed functional attributes, a range of soluble metabolic and immune indices, and gene expression markers in males with or without evidence of metabolic dysregulation (MetDys). Methods : This case-control study included healthy males (n=15; 41.9±11.7 years; body mass index [BMI], 22.9±1.2 kg/m2) and males with evidence of MetDys (n=14; 46.6±10.0 years; BMI, 35.1±3.3 kg/m2) who provided blood and faecal samples for assessment of a range of metabolic and immune markers and microbial composition using 16S rRNA gene sequencing. Metagenomic functions were imputed from microbial sequence data for analysis. Results : In addition to elevated values in a range of traditional metabolic, adipokine and inflammatory indices in the MetDys group, 23 immunomodulatory genes were significantly altered in the MetDys group. Overall microbial diversity did not differ between groups; however, a trend for a higher relative abundance of the Bacteroidetes (P=0.06) and a lower relative abundance of the Verrucomicrobia (P=0.09) phyla was noted in the MetDys group. Using both family- and genera-level classifications, a partial least square discriminant analysis revealed unique microbial signatures between the groups. Conclusion : These findings confirm the need for ongoing investigations in human clinical cohorts to further resolve the relationships between the gut microbiota and metabolic and immune markers and risk for metabolic disease.http://www.jomes.org/journal/view.html?doi=10.7570/jomes23022microbiotametabolic diseasesobesityinflammation |
| spellingShingle | Kyle M. Hatton-Jones Nicholas P. West Mike W.C. Thang Pin-Yen Chen Peter Davoren Allan W. Cripps Amanda J. Cox Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation Journal of Obesity & Metabolic Syndrome microbiota metabolic diseases obesity inflammation |
| title | Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation |
| title_full | Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation |
| title_fullStr | Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation |
| title_full_unstemmed | Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation |
| title_short | Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic Dysregulation |
| title_sort | gut microbiome and metabolic and immune indices in males with or without evidence of metabolic dysregulation |
| topic | microbiota metabolic diseases obesity inflammation |
| url | http://www.jomes.org/journal/view.html?doi=10.7570/jomes23022 |
| work_keys_str_mv | AT kylemhattonjones gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT nicholaspwest gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT mikewcthang gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT pinyenchen gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT peterdavoren gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT allanwcripps gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation AT amandajcox gutmicrobiomeandmetabolicandimmuneindicesinmaleswithorwithoutevidenceofmetabolicdysregulation |