Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism.
Calorie restriction (CR) is an intervention known to extend the lifespan of a wide variety of organisms. In S. cerevisiae, chronological lifespan is prolonged by decreasing glucose availability in the culture media, a model for CR. The mechanism has been proposed to involve an increase in the oxidat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3569431?pdf=render |
| _version_ | 1818553302751641600 |
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| author | Erich B Tahara Fernanda M Cunha Thiago O Basso Bianca E Della Bianca Andreas K Gombert Alicia J Kowaltowski |
| author_facet | Erich B Tahara Fernanda M Cunha Thiago O Basso Bianca E Della Bianca Andreas K Gombert Alicia J Kowaltowski |
| author_sort | Erich B Tahara |
| collection | DOAJ |
| description | Calorie restriction (CR) is an intervention known to extend the lifespan of a wide variety of organisms. In S. cerevisiae, chronological lifespan is prolonged by decreasing glucose availability in the culture media, a model for CR. The mechanism has been proposed to involve an increase in the oxidative (versus fermentative) metabolism of glucose. Here, we measured wild-type and respiratory incompetent (ρ(0)) S. cerevisiae biomass formation, pH, oxygen and glucose consumption, and the evolution of ethanol, glycerol, acetate, pyruvate and succinate levels during the course of 28 days of chronological aging, aiming to identify metabolic changes responsible for the effects of CR. The concomitant and quantitative measurements allowed for calculations of conversion factors between different pairs of substrates and products, maximum specific substrate consumption and product formation rates and maximum specific growth rates. Interestingly, we found that the limitation of glucose availability in CR S. cerevisiae cultures hysteretically increases oxygen consumption rates many hours after the complete exhaustion of glucose from the media. Surprisingly, glucose-to-ethanol conversion and cellular growth supported by glucose were not quantitatively altered by CR. Instead, we found that CR primed the cells for earlier, faster and more efficient metabolism of respiratory substrates, especially ethanol. Since lifespan-enhancing effects of CR are absent in respiratory incompetent ρ(0) cells, we propose that the hysteretic effect of glucose limitation on oxidative metabolism is central toward chronological lifespan extension by CR in this yeast. |
| first_indexed | 2024-12-12T09:24:07Z |
| format | Article |
| id | doaj.art-9084b37352c24951b02ea4b045203075 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T09:24:07Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9084b37352c24951b02ea4b0452030752022-12-22T00:29:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5638810.1371/journal.pone.0056388Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism.Erich B TaharaFernanda M CunhaThiago O BassoBianca E Della BiancaAndreas K GombertAlicia J KowaltowskiCalorie restriction (CR) is an intervention known to extend the lifespan of a wide variety of organisms. In S. cerevisiae, chronological lifespan is prolonged by decreasing glucose availability in the culture media, a model for CR. The mechanism has been proposed to involve an increase in the oxidative (versus fermentative) metabolism of glucose. Here, we measured wild-type and respiratory incompetent (ρ(0)) S. cerevisiae biomass formation, pH, oxygen and glucose consumption, and the evolution of ethanol, glycerol, acetate, pyruvate and succinate levels during the course of 28 days of chronological aging, aiming to identify metabolic changes responsible for the effects of CR. The concomitant and quantitative measurements allowed for calculations of conversion factors between different pairs of substrates and products, maximum specific substrate consumption and product formation rates and maximum specific growth rates. Interestingly, we found that the limitation of glucose availability in CR S. cerevisiae cultures hysteretically increases oxygen consumption rates many hours after the complete exhaustion of glucose from the media. Surprisingly, glucose-to-ethanol conversion and cellular growth supported by glucose were not quantitatively altered by CR. Instead, we found that CR primed the cells for earlier, faster and more efficient metabolism of respiratory substrates, especially ethanol. Since lifespan-enhancing effects of CR are absent in respiratory incompetent ρ(0) cells, we propose that the hysteretic effect of glucose limitation on oxidative metabolism is central toward chronological lifespan extension by CR in this yeast.http://europepmc.org/articles/PMC3569431?pdf=render |
| spellingShingle | Erich B Tahara Fernanda M Cunha Thiago O Basso Bianca E Della Bianca Andreas K Gombert Alicia J Kowaltowski Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. PLoS ONE |
| title | Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. |
| title_full | Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. |
| title_fullStr | Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. |
| title_full_unstemmed | Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. |
| title_short | Calorie restriction hysteretically primes aging Saccharomyces cerevisiae toward more effective oxidative metabolism. |
| title_sort | calorie restriction hysteretically primes aging saccharomyces cerevisiae toward more effective oxidative metabolism |
| url | http://europepmc.org/articles/PMC3569431?pdf=render |
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