Chemokine localization in bronchial angiogenesis.
Angiogenesis in the lung involves the systemic bronchial vasculature and becomes prominent when chronic inflammation prevails. Mechanisms for neovascularization following pulmonary ischemia include growth factor transit from ischemic parenchyma to upstream bronchial arteries, inflammatory cell migra...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3679055?pdf=render |
| _version_ | 1818096876029739008 |
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| author | Maria Grazia Perino Aigul Moldobaeva John Jenkins Elizabeth M Wagner |
| author_facet | Maria Grazia Perino Aigul Moldobaeva John Jenkins Elizabeth M Wagner |
| author_sort | Maria Grazia Perino |
| collection | DOAJ |
| description | Angiogenesis in the lung involves the systemic bronchial vasculature and becomes prominent when chronic inflammation prevails. Mechanisms for neovascularization following pulmonary ischemia include growth factor transit from ischemic parenchyma to upstream bronchial arteries, inflammatory cell migration/recruitment through the perfusing artery, and paracrine effects of lung cells within the left bronchus, the niche where arteriogenesis takes place. We analyzed left lung bronchoalveolar lavage (BAL) fluid and left bronchus homogenates after left pulmonary artery ligation (LPAL) in rats, immediately after the onset of ischemia (0 h), 6 h and 24 h later. Additionally, we tested the effectiveness of dexamethasone on decreasing inflammation (0-24 h LPAL) and angiogenesis at early (3 d LPAL; bronchial endothelial proliferation) and late (14 d LPAL; blood flow) stages. After LPAL (6 h), BAL protein, total inflammatory cells, macrophages, and polymorphonuclear cells increased significantly. In parallel, pro-angiogenic CXC chemokines increased in BAL and the left main-stem bronchus (CXCL1) or only within the bronchus (CXCL2). Dexamethasone treatment reduced total BAL protein, inflammatory cells (total and polymorphonuclear cells), and CXCL1 but not CXCL2 in BAL. By contrast, no decrease was seen in either chemokine within the bronchial tissue, in proliferating bronchial endothelial cells, or in systemic perfusion of the left lung. Our results confirm the presence of CXC chemokines within BAL fluid as well as within the left mainstem bronchus. Despite significant reduction in lung injury and inflammation with dexamethasone treatment, chemokine expression within the bronchial tissue as well as angiogenesis were not affected. Our results suggest that early changes within the bronchial niche contribute to subsequent neovascularization during pulmonary ischemia. |
| first_indexed | 2024-12-10T23:11:35Z |
| format | Article |
| id | doaj.art-9085afc3909e4fdb85bbe8926a0b0ace |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T23:11:35Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9085afc3909e4fdb85bbe8926a0b0ace2022-12-22T01:29:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6643210.1371/journal.pone.0066432Chemokine localization in bronchial angiogenesis.Maria Grazia PerinoAigul MoldobaevaJohn JenkinsElizabeth M WagnerAngiogenesis in the lung involves the systemic bronchial vasculature and becomes prominent when chronic inflammation prevails. Mechanisms for neovascularization following pulmonary ischemia include growth factor transit from ischemic parenchyma to upstream bronchial arteries, inflammatory cell migration/recruitment through the perfusing artery, and paracrine effects of lung cells within the left bronchus, the niche where arteriogenesis takes place. We analyzed left lung bronchoalveolar lavage (BAL) fluid and left bronchus homogenates after left pulmonary artery ligation (LPAL) in rats, immediately after the onset of ischemia (0 h), 6 h and 24 h later. Additionally, we tested the effectiveness of dexamethasone on decreasing inflammation (0-24 h LPAL) and angiogenesis at early (3 d LPAL; bronchial endothelial proliferation) and late (14 d LPAL; blood flow) stages. After LPAL (6 h), BAL protein, total inflammatory cells, macrophages, and polymorphonuclear cells increased significantly. In parallel, pro-angiogenic CXC chemokines increased in BAL and the left main-stem bronchus (CXCL1) or only within the bronchus (CXCL2). Dexamethasone treatment reduced total BAL protein, inflammatory cells (total and polymorphonuclear cells), and CXCL1 but not CXCL2 in BAL. By contrast, no decrease was seen in either chemokine within the bronchial tissue, in proliferating bronchial endothelial cells, or in systemic perfusion of the left lung. Our results confirm the presence of CXC chemokines within BAL fluid as well as within the left mainstem bronchus. Despite significant reduction in lung injury and inflammation with dexamethasone treatment, chemokine expression within the bronchial tissue as well as angiogenesis were not affected. Our results suggest that early changes within the bronchial niche contribute to subsequent neovascularization during pulmonary ischemia.http://europepmc.org/articles/PMC3679055?pdf=render |
| spellingShingle | Maria Grazia Perino Aigul Moldobaeva John Jenkins Elizabeth M Wagner Chemokine localization in bronchial angiogenesis. PLoS ONE |
| title | Chemokine localization in bronchial angiogenesis. |
| title_full | Chemokine localization in bronchial angiogenesis. |
| title_fullStr | Chemokine localization in bronchial angiogenesis. |
| title_full_unstemmed | Chemokine localization in bronchial angiogenesis. |
| title_short | Chemokine localization in bronchial angiogenesis. |
| title_sort | chemokine localization in bronchial angiogenesis |
| url | http://europepmc.org/articles/PMC3679055?pdf=render |
| work_keys_str_mv | AT mariagraziaperino chemokinelocalizationinbronchialangiogenesis AT aigulmoldobaeva chemokinelocalizationinbronchialangiogenesis AT johnjenkins chemokinelocalizationinbronchialangiogenesis AT elizabethmwagner chemokinelocalizationinbronchialangiogenesis |