Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage
Seed vigour and viability, synchronous fluorescence spectroscopy, and proteomic profiles were analysed in field bean (Vicia faba var. minor) (<i>Vicia faba</i> var. <i>minor</i>) seeds (two cultivars) subjected to dry storage at −14 °C or +20 °C for 30 years. The seeds stored...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/10/11/545 |
| _version_ | 1827702344263925760 |
|---|---|
| author | Agnieszka I. Piotrowicz-Cieślak Magdalena Krupka Dariusz J. Michalczyk Bogdan Smyk Hanna Grajek Wiesław Podyma Katarzyna Głowacka |
| author_facet | Agnieszka I. Piotrowicz-Cieślak Magdalena Krupka Dariusz J. Michalczyk Bogdan Smyk Hanna Grajek Wiesław Podyma Katarzyna Głowacka |
| author_sort | Agnieszka I. Piotrowicz-Cieślak |
| collection | DOAJ |
| description | Seed vigour and viability, synchronous fluorescence spectroscopy, and proteomic profiles were analysed in field bean (Vicia faba var. minor) (<i>Vicia faba</i> var. <i>minor</i>) seeds (two cultivars) subjected to dry storage at −14 °C or +20 °C for 30 years. The seeds stored at −14 °C retained very high germinability (91–98%) until the end of the experiments, while seeds from the same lots but stored at room temperature completely lost viability. The deterioration of seeds stored at +20 °C was also manifested by a vast (4- to 6-fold) increase in leachate electroconductivity, and the changes in synchronous spectra and proteomic profiles. To carry out detailed analyses of seed proteins, protein extracts were pre-purified and divided into albumin, vicilin, and legumin. Only one protein, superoxide dismutase, was more abundant in deteriorated seeds (of one cultivar) compared to the high vigour seeds. The results show that seed deterioration strongly and specifically affects the contents of some storage proteins. Moreover, the colour of seed coats changes gradually, and seeds stored at −14 °C were light brown, while those constantly exposed to +20 °C turned black. Synchronous fluorescence spectroscopy showed that this change of colour was caused by formation of oxidized and condensed phenols and that the phenol content in seed coats decreased parallel to seed deterioration. |
| first_indexed | 2024-03-10T14:53:00Z |
| format | Article |
| id | doaj.art-908611fff90a45568668d67ea42de86b |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T14:53:00Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-908611fff90a45568668d67ea42de86b2023-11-20T20:49:14ZengMDPI AGAgriculture2077-04722020-11-01101154510.3390/agriculture10110545Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of StorageAgnieszka I. Piotrowicz-Cieślak0Magdalena Krupka1Dariusz J. Michalczyk2Bogdan Smyk3Hanna Grajek4Wiesław Podyma5Katarzyna Głowacka6Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn Oczapowskiego 1A, 10-718 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn Oczapowskiego 1A, 10-718 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn Oczapowskiego 1A, 10-718 Olsztyn, PolandDepartment of Physics and Biophysics, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, PolandDepartment of Physics and Biophysics, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, PolandPlant Breeding and Acclimatization Institute (IHAR), National Research Institute, Radzików, 05-870 Błonie, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn Oczapowskiego 1A, 10-718 Olsztyn, PolandSeed vigour and viability, synchronous fluorescence spectroscopy, and proteomic profiles were analysed in field bean (Vicia faba var. minor) (<i>Vicia faba</i> var. <i>minor</i>) seeds (two cultivars) subjected to dry storage at −14 °C or +20 °C for 30 years. The seeds stored at −14 °C retained very high germinability (91–98%) until the end of the experiments, while seeds from the same lots but stored at room temperature completely lost viability. The deterioration of seeds stored at +20 °C was also manifested by a vast (4- to 6-fold) increase in leachate electroconductivity, and the changes in synchronous spectra and proteomic profiles. To carry out detailed analyses of seed proteins, protein extracts were pre-purified and divided into albumin, vicilin, and legumin. Only one protein, superoxide dismutase, was more abundant in deteriorated seeds (of one cultivar) compared to the high vigour seeds. The results show that seed deterioration strongly and specifically affects the contents of some storage proteins. Moreover, the colour of seed coats changes gradually, and seeds stored at −14 °C were light brown, while those constantly exposed to +20 °C turned black. Synchronous fluorescence spectroscopy showed that this change of colour was caused by formation of oxidized and condensed phenols and that the phenol content in seed coats decreased parallel to seed deterioration.https://www.mdpi.com/2077-0472/10/11/545albuminleguminlong-term storage<i>Vicia faba</i> var. <i>minor</i> seedsvicilintestae |
| spellingShingle | Agnieszka I. Piotrowicz-Cieślak Magdalena Krupka Dariusz J. Michalczyk Bogdan Smyk Hanna Grajek Wiesław Podyma Katarzyna Głowacka Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage Agriculture albumin legumin long-term storage <i>Vicia faba</i> var. <i>minor</i> seeds vicilin testae |
| title | Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage |
| title_full | Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage |
| title_fullStr | Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage |
| title_full_unstemmed | Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage |
| title_short | Physiological Characteristics of Field Bean Seeds (<i>Vicia faba</i> var. <i>minor</i>) Subjected to 30 Years of Storage |
| title_sort | physiological characteristics of field bean seeds i vicia faba i var i minor i subjected to 30 years of storage |
| topic | albumin legumin long-term storage <i>Vicia faba</i> var. <i>minor</i> seeds vicilin testae |
| url | https://www.mdpi.com/2077-0472/10/11/545 |
| work_keys_str_mv | AT agnieszkaipiotrowiczcieslak physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT magdalenakrupka physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT dariuszjmichalczyk physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT bogdansmyk physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT hannagrajek physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT wiesławpodyma physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage AT katarzynagłowacka physiologicalcharacteristicsoffieldbeanseedsiviciafabaivariminorisubjectedto30yearsofstorage |