Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase
<p>Abstract</p> <p>Background</p> <p>Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material. Consolidated bioprocessing (CBP), combining cellula...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2013-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://www.microbialcellfactories.com/content/12/1/14 |
| _version_ | 1828470711272865792 |
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| author | Kim Sujin Baek Seung-Ho Lee Kyusung Hahn Ji-Sook |
| author_facet | Kim Sujin Baek Seung-Ho Lee Kyusung Hahn Ji-Sook |
| author_sort | Kim Sujin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material. Consolidated bioprocessing (CBP), combining cellulase production, saccharification, and fermentation into one step, has been proposed as the most efficient way to reduce the production cost of cellulosic bioethanol. In this study, we developed a cellulolytic yeast consortium for CBP, based on the surface display of cellulosome structure, mimicking the cellulolytic bacterium, <it>Clostridium thermocellum</it>.</p> <p>Results</p> <p>We designed a cellulolytic yeast consortium composed of four different yeast strains capable of either displaying a scaffoldin (mini CipA) containing three cohesin domains derived from C. <it>thermocellum</it>, or secreting one of the three types of cellulases, <it>C. thermocellum</it> CelA (endoglucanase) containing its own dockerin, <it>Trichoderma reesei</it> CBHII (exoglucanase) fused with an exogenous dockerin from <it>C. thermocellum</it>, or <it>Aspergillus aculeatus</it> BGLI (β-glucosidase). The secreted dockerin-containing enzymes, CelA and CBHI, were randomly assembled to the surface-displayed mini CipA via cohesin-dockerin interactions. On the other hand, BGLI was independently assembled to the cell surface since we newly found that it already has a cell adhesion characteristic. We optimized the cellulosome activity and ethanol production by controlling the combination ratio among the four yeast strains. A mixture of cells with the optimized mini CipA:CelA:CBHII:BGLI ratio of 2:3:3:0.53 produced 1.80 g/l ethanol after 94 h, indicating about 20% increase compared with a consortium composed of an equal amount of each cell type (1.48 g/l).</p> <p>Conclusions</p> <p>We produced cellulosic ethanol using a cellulolytic yeast consortium, which is composed of cells displaying mini cellulosomes generated via random assembly of CelA and CBHII to a mini CipA, and cells displaying BGLI independently. One of the advantages of this system is that ethanol production can be easily optimized by simply changing the combination ratio of the different populations. In addition, there is no limitation on the number of enzymes to be incorporated into this cellulosome structure. Not only cellulases used in this study, but also any other enzymes, including cellulases and hemicellulases, could be applied just by fusing dockerin domains to the enzymes.</p> |
| first_indexed | 2024-12-11T04:59:28Z |
| format | Article |
| id | doaj.art-9086348b6afc417b9060c665dd232fd0 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-11T04:59:28Z |
| publishDate | 2013-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-9086348b6afc417b9060c665dd232fd02022-12-22T01:20:12ZengBMCMicrobial Cell Factories1475-28592013-02-011211410.1186/1475-2859-12-14Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidaseKim SujinBaek Seung-HoLee KyusungHahn Ji-Sook<p>Abstract</p> <p>Background</p> <p>Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material. Consolidated bioprocessing (CBP), combining cellulase production, saccharification, and fermentation into one step, has been proposed as the most efficient way to reduce the production cost of cellulosic bioethanol. In this study, we developed a cellulolytic yeast consortium for CBP, based on the surface display of cellulosome structure, mimicking the cellulolytic bacterium, <it>Clostridium thermocellum</it>.</p> <p>Results</p> <p>We designed a cellulolytic yeast consortium composed of four different yeast strains capable of either displaying a scaffoldin (mini CipA) containing three cohesin domains derived from C. <it>thermocellum</it>, or secreting one of the three types of cellulases, <it>C. thermocellum</it> CelA (endoglucanase) containing its own dockerin, <it>Trichoderma reesei</it> CBHII (exoglucanase) fused with an exogenous dockerin from <it>C. thermocellum</it>, or <it>Aspergillus aculeatus</it> BGLI (β-glucosidase). The secreted dockerin-containing enzymes, CelA and CBHI, were randomly assembled to the surface-displayed mini CipA via cohesin-dockerin interactions. On the other hand, BGLI was independently assembled to the cell surface since we newly found that it already has a cell adhesion characteristic. We optimized the cellulosome activity and ethanol production by controlling the combination ratio among the four yeast strains. A mixture of cells with the optimized mini CipA:CelA:CBHII:BGLI ratio of 2:3:3:0.53 produced 1.80 g/l ethanol after 94 h, indicating about 20% increase compared with a consortium composed of an equal amount of each cell type (1.48 g/l).</p> <p>Conclusions</p> <p>We produced cellulosic ethanol using a cellulolytic yeast consortium, which is composed of cells displaying mini cellulosomes generated via random assembly of CelA and CBHII to a mini CipA, and cells displaying BGLI independently. One of the advantages of this system is that ethanol production can be easily optimized by simply changing the combination ratio of the different populations. In addition, there is no limitation on the number of enzymes to be incorporated into this cellulosome structure. Not only cellulases used in this study, but also any other enzymes, including cellulases and hemicellulases, could be applied just by fusing dockerin domains to the enzymes.</p>http://www.microbialcellfactories.com/content/12/1/14Cellulosic bioethanolCellulosomeConsolidated bioprocessingYeast surface display |
| spellingShingle | Kim Sujin Baek Seung-Ho Lee Kyusung Hahn Ji-Sook Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase Microbial Cell Factories Cellulosic bioethanol Cellulosome Consolidated bioprocessing Yeast surface display |
| title | Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase |
| title_full | Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase |
| title_fullStr | Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase |
| title_full_unstemmed | Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase |
| title_short | Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase |
| title_sort | cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β glucosidase |
| topic | Cellulosic bioethanol Cellulosome Consolidated bioprocessing Yeast surface display |
| url | http://www.microbialcellfactories.com/content/12/1/14 |
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