Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid
Abstract Ferrocene (Fc) is a widely used metallocene, with well‐characterized redox behaviour that is vastly used in a variety of applications. Ferrocene‐coupling to peptides renders the resulting bioorganometallic peptide conjugate redox active and sensitive to changes in the chemical environment....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-11-01
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| Series: | ChemElectroChem |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/celc.202300301 |
| _version_ | 1827752534751576064 |
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| author | Tsuimy Shao Junyan Wang Dr. Meissam Noroozifar Prof. Dr. Heinz‐Bernhard Kraatz |
| author_facet | Tsuimy Shao Junyan Wang Dr. Meissam Noroozifar Prof. Dr. Heinz‐Bernhard Kraatz |
| author_sort | Tsuimy Shao |
| collection | DOAJ |
| description | Abstract Ferrocene (Fc) is a widely used metallocene, with well‐characterized redox behaviour that is vastly used in a variety of applications. Ferrocene‐coupling to peptides renders the resulting bioorganometallic peptide conjugate redox active and sensitive to changes in the chemical environment. The disulfide‐linked ferrocene peptide conjugate (FcCO‐Phe‐Cys‐OH)2 was synthesized and characterized by NMR, MS, and FT‐IR spectroscopies and then exploited for the detection of ascorbic acid. Here we demonstrate that the Cu(II) complex of this peptide conjugate allows the detection of ascorbic acid with a limit of detection of 0.02 μM with a linear range of 0.1–25 μM. This is the first report of the use of a peptide metal complex for direct modification of a glass carbon electrode and its application for ascorbic acid detection and quantification, including in commercial beverages. The modified GCE exhibited excellent selectivity, stability, and sensitivity. |
| first_indexed | 2024-03-11T07:18:41Z |
| format | Article |
| id | doaj.art-9086432ade7e43c080cde4d954b4b4c6 |
| institution | Directory Open Access Journal |
| issn | 2196-0216 |
| language | English |
| last_indexed | 2024-03-11T07:18:41Z |
| publishDate | 2023-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemElectroChem |
| spelling | doaj.art-9086432ade7e43c080cde4d954b4b4c62023-11-17T08:08:58ZengWiley-VCHChemElectroChem2196-02162023-11-011022n/an/a10.1002/celc.202300301Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic AcidTsuimy Shao0Junyan Wang1Dr. Meissam Noroozifar2Prof. Dr. Heinz‐Bernhard Kraatz3Department of Physical and Environmental Sciences University of Toronto Scarborough 1265 Military Trail Toronto M1C 1A4 Ontario CanadaDepartment of Physical and Environmental Sciences University of Toronto Scarborough 1265 Military Trail Toronto M1C 1A4 Ontario CanadaDepartment of Physical and Environmental Sciences University of Toronto Scarborough 1265 Military Trail Toronto M1C 1A4 Ontario CanadaDepartment of Physical and Environmental Sciences University of Toronto Scarborough 1265 Military Trail Toronto M1C 1A4 Ontario CanadaAbstract Ferrocene (Fc) is a widely used metallocene, with well‐characterized redox behaviour that is vastly used in a variety of applications. Ferrocene‐coupling to peptides renders the resulting bioorganometallic peptide conjugate redox active and sensitive to changes in the chemical environment. The disulfide‐linked ferrocene peptide conjugate (FcCO‐Phe‐Cys‐OH)2 was synthesized and characterized by NMR, MS, and FT‐IR spectroscopies and then exploited for the detection of ascorbic acid. Here we demonstrate that the Cu(II) complex of this peptide conjugate allows the detection of ascorbic acid with a limit of detection of 0.02 μM with a linear range of 0.1–25 μM. This is the first report of the use of a peptide metal complex for direct modification of a glass carbon electrode and its application for ascorbic acid detection and quantification, including in commercial beverages. The modified GCE exhibited excellent selectivity, stability, and sensitivity.https://doi.org/10.1002/celc.202300301Ferrocene peptidebioorganometallicascorbic acidsensorvoltammetry |
| spellingShingle | Tsuimy Shao Junyan Wang Dr. Meissam Noroozifar Prof. Dr. Heinz‐Bernhard Kraatz Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid ChemElectroChem Ferrocene peptide bioorganometallic ascorbic acid sensor voltammetry |
| title | Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid |
| title_full | Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid |
| title_fullStr | Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid |
| title_full_unstemmed | Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid |
| title_short | Ferrocene‐Peptide Conjugate for the Detection and Quantification of Ascorbic Acid |
| title_sort | ferrocene peptide conjugate for the detection and quantification of ascorbic acid |
| topic | Ferrocene peptide bioorganometallic ascorbic acid sensor voltammetry |
| url | https://doi.org/10.1002/celc.202300301 |
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