Efficient Generation of P53 Biallelic Mutations in Diannan Miniature Pigs Using RNA-Guided Base Editing

Efficient Generation of P53 Biallelic Mutations in Diannan Miniature Pigs Using RNA-Guided Base Editing

The base editing 3 (BE3) system, a single-base gene editing technology developed using CRISPR/Cas9n, has a broad range of applications for human disease model construction and gene therapy, as it is highly efficient, accurate, and non-destructive. P53 mutations are present in more than 50% of human...

Full description

Bibliographic Details
Main Authors: Honghui Li, Wenmin Cheng, Bowei Chen, Shaoxia Pu, Ninglin Fan, Xiaolin Zhang, Deling Jiao, Dejia Shi, Jianxiong Guo, Zhuo Li, Yubo Qing, Baoyu Jia, Hong-Ye Zhao, Hong-Jiang Wei
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Life
Subjects:
cancer
P53 gene
BE3 system
SCNT
point mutation
Online Access:https://www.mdpi.com/2075-1729/11/12/1417

Internet

https://www.mdpi.com/2075-1729/11/12/1417

Similar Items