Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors
In this paper, the characterization of the main techniques and transducers employed to measure local and global strains induced by uniaxial loading of murine tibiae is presented. Micro strain gauges and digital image correlation (DIC) were tested to measure local strains, while a moving coil motor-b...
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MDPI AG
2019-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/23/5109 |
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| author | Emanuele Rizzuto Barbara Peruzzi Mariagrazia Giudice Enrica Urciuoli Erika Pittella Emanuele Piuzzi Antonio Musarò Zaccaria Del Prete |
| author_facet | Emanuele Rizzuto Barbara Peruzzi Mariagrazia Giudice Enrica Urciuoli Erika Pittella Emanuele Piuzzi Antonio Musarò Zaccaria Del Prete |
| author_sort | Emanuele Rizzuto |
| collection | DOAJ |
| description | In this paper, the characterization of the main techniques and transducers employed to measure local and global strains induced by uniaxial loading of murine tibiae is presented. Micro strain gauges and digital image correlation (DIC) were tested to measure local strains, while a moving coil motor-based length transducer was employed to measure relative global shortening. Local strain is the crucial parameter to be measured when dealing with bone cell mechanotransduction, so we characterized these techniques in the experimental conditions known to activate cell mechanosensing in vivo. The experimental tests were performed using tibia samples excised from twenty-two C57BL/6 mice. To evaluate measurement repeatability we computed the standard deviation of ten repetitive compressions to the mean value. This value was lower than 3% for micro strain gauges, and in the range of 7%−10% for DIC and the length transducer. The coefficient of variation, i.e., the standard deviation to the mean value, was about 35% for strain gauges and the length transducer, and about 40% for DIC. These results provided a comprehensive characterization of three methodologies for local and global bone strain measurement, suggesting a possible field of application on the basis of their advantages and limitations. |
| first_indexed | 2024-04-14T01:44:09Z |
| format | Article |
| id | doaj.art-908f641a24924e94829f6982ca588473 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T01:44:09Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-908f641a24924e94829f6982ca5884732022-12-22T02:19:37ZengMDPI AGSensors1424-82202019-11-011923510910.3390/s19235109s19235109Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different SensorsEmanuele Rizzuto0Barbara Peruzzi1Mariagrazia Giudice2Enrica Urciuoli3Erika Pittella4Emanuele Piuzzi5Antonio Musarò6Zaccaria Del Prete7Department of Mechanical and Aerospace Engineering, Sapienza University of Rome, 00184 Rome, ItalyMultifactorial Disease and Complex Phenotype Research Area, Children’s Hospital Bambino Gesù, 00146 Rome, ItalySapienza University of Rome, 00185 Rome, ItalyMultifactorial Disease and Complex Phenotype Research Area, Children’s Hospital Bambino Gesù, 00146 Rome, ItalyDepartment of Information, Telecommunication and Electronic Engineering, Sapienza University of Rome, 00184 Rome, ItalyDepartment of Information, Telecommunication and Electronic Engineering, Sapienza University of Rome, 00184 Rome, ItalyInstitute Pasteur Cenci-Bolognetti, DAHFMO-Unit of Histology and Medical Embryology, IIM, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Mechanical and Aerospace Engineering, Sapienza University of Rome, 00184 Rome, ItalyIn this paper, the characterization of the main techniques and transducers employed to measure local and global strains induced by uniaxial loading of murine tibiae is presented. Micro strain gauges and digital image correlation (DIC) were tested to measure local strains, while a moving coil motor-based length transducer was employed to measure relative global shortening. Local strain is the crucial parameter to be measured when dealing with bone cell mechanotransduction, so we characterized these techniques in the experimental conditions known to activate cell mechanosensing in vivo. The experimental tests were performed using tibia samples excised from twenty-two C57BL/6 mice. To evaluate measurement repeatability we computed the standard deviation of ten repetitive compressions to the mean value. This value was lower than 3% for micro strain gauges, and in the range of 7%−10% for DIC and the length transducer. The coefficient of variation, i.e., the standard deviation to the mean value, was about 35% for strain gauges and the length transducer, and about 40% for DIC. These results provided a comprehensive characterization of three methodologies for local and global bone strain measurement, suggesting a possible field of application on the basis of their advantages and limitations.https://www.mdpi.com/1424-8220/19/23/5109strain measurementdigital image correlation (dic)strain gaugestissue biomechanicsmechanotransductionuniaxial loadingbone tissue |
| spellingShingle | Emanuele Rizzuto Barbara Peruzzi Mariagrazia Giudice Enrica Urciuoli Erika Pittella Emanuele Piuzzi Antonio Musarò Zaccaria Del Prete Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors Sensors strain measurement digital image correlation (dic) strain gauges tissue biomechanics mechanotransduction uniaxial loading bone tissue |
| title | Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors |
| title_full | Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors |
| title_fullStr | Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors |
| title_full_unstemmed | Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors |
| title_short | Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors |
| title_sort | detection of the strains induced in murine tibias by ex vivo uniaxial loading with different sensors |
| topic | strain measurement digital image correlation (dic) strain gauges tissue biomechanics mechanotransduction uniaxial loading bone tissue |
| url | https://www.mdpi.com/1424-8220/19/23/5109 |
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