Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish
Summary: Animal development proceeds in the presence of intimate microbial associations, but the extent to which different host cells across the body respond to resident microbes remains to be fully explored. Using the vertebrate model organism, the larval zebrafish, we assessed transcriptional resp...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723001067 |
| _version_ | 1828032511566938112 |
|---|---|
| author | Michelle S. Massaquoi Garth L. Kong Daisy Chilin-Fuentes Julia S. Ngo Patrick F. Horve Ellie Melancon M. Kristina Hamilton Judith S. Eisen Karen Guillemin |
| author_facet | Michelle S. Massaquoi Garth L. Kong Daisy Chilin-Fuentes Julia S. Ngo Patrick F. Horve Ellie Melancon M. Kristina Hamilton Judith S. Eisen Karen Guillemin |
| author_sort | Michelle S. Massaquoi |
| collection | DOAJ |
| description | Summary: Animal development proceeds in the presence of intimate microbial associations, but the extent to which different host cells across the body respond to resident microbes remains to be fully explored. Using the vertebrate model organism, the larval zebrafish, we assessed transcriptional responses to the microbiota across the entire body at single-cell resolution. We find that cell types across the body, not limited to tissues at host-microbe interfaces, respond to the microbiota. Responses are cell-type-specific, but across many tissues the microbiota enhances cell proliferation, increases metabolism, and stimulates a diversity of cellular activities, revealing roles for the microbiota in promoting developmental plasticity. This work provides a resource for exploring transcriptional responses to the microbiota across all cell types of the vertebrate body and generating new hypotheses about the interactions between vertebrate hosts and their microbiota. |
| first_indexed | 2024-04-10T15:06:33Z |
| format | Article |
| id | doaj.art-9091cf8dfc264a89bde72fd8d7891eb1 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-10T15:06:33Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-9091cf8dfc264a89bde72fd8d7891eb12023-02-15T04:27:49ZengElsevierCell Reports2211-12472023-02-01422112095Cell-type-specific responses to the microbiota across all tissues of the larval zebrafishMichelle S. Massaquoi0Garth L. Kong1Daisy Chilin-Fuentes2Julia S. Ngo3Patrick F. Horve4Ellie Melancon5M. Kristina Hamilton6Judith S. Eisen7Karen Guillemin8Institute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USA; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR 97402, USA; Thermo Fisher Scientific, 22025 20th Avenue SE, Bothell, WA 98021, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USAInstitute of Neuroscience, University of Oregon, 1254 University of Oregon, Eugene, OR 97403, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USA; Institute of Neuroscience, University of Oregon, 1254 University of Oregon, Eugene, OR 97403, USA; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR 97402, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USA; Institute of Neuroscience, University of Oregon, 1254 University of Oregon, Eugene, OR 97403, USAInstitute of Molecular Biology, University of Oregon, 1318 Franklin Boulevard, Eugene, OR 97403, USA; Humans and the Microbiome Program, CIFAR, Toronto, ON M5G 1M1, Canada; Corresponding authorSummary: Animal development proceeds in the presence of intimate microbial associations, but the extent to which different host cells across the body respond to resident microbes remains to be fully explored. Using the vertebrate model organism, the larval zebrafish, we assessed transcriptional responses to the microbiota across the entire body at single-cell resolution. We find that cell types across the body, not limited to tissues at host-microbe interfaces, respond to the microbiota. Responses are cell-type-specific, but across many tissues the microbiota enhances cell proliferation, increases metabolism, and stimulates a diversity of cellular activities, revealing roles for the microbiota in promoting developmental plasticity. This work provides a resource for exploring transcriptional responses to the microbiota across all cell types of the vertebrate body and generating new hypotheses about the interactions between vertebrate hosts and their microbiota.http://www.sciencedirect.com/science/article/pii/S2211124723001067CP: MicrobiologyCP: Immunology |
| spellingShingle | Michelle S. Massaquoi Garth L. Kong Daisy Chilin-Fuentes Julia S. Ngo Patrick F. Horve Ellie Melancon M. Kristina Hamilton Judith S. Eisen Karen Guillemin Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish Cell Reports CP: Microbiology CP: Immunology |
| title | Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish |
| title_full | Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish |
| title_fullStr | Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish |
| title_full_unstemmed | Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish |
| title_short | Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish |
| title_sort | cell type specific responses to the microbiota across all tissues of the larval zebrafish |
| topic | CP: Microbiology CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723001067 |
| work_keys_str_mv | AT michellesmassaquoi celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT garthlkong celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT daisychilinfuentes celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT juliasngo celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT patrickfhorve celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT elliemelancon celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT mkristinahamilton celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT judithseisen celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish AT karenguillemin celltypespecificresponsestothemicrobiotaacrossalltissuesofthelarvalzebrafish |