Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus
Objective: To explore the effects of the extract of Sanchen powder (ESCP) combined with vancomycin on methicillin-resistant Staphylococcus aureus (MRSA) planktonic cells, biofilms, and virulence factors. Methods: The herbs in Sanchen powder (SCP) were extracted separately with 50% ethanol. Then, the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754822000229 |
| _version_ | 1797756651710709760 |
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| author | Jiajia Fan Honghao Sun Yuanyuan Liu Xin Li Huichao Wu Xiaoqiao Ren |
| author_facet | Jiajia Fan Honghao Sun Yuanyuan Liu Xin Li Huichao Wu Xiaoqiao Ren |
| author_sort | Jiajia Fan |
| collection | DOAJ |
| description | Objective: To explore the effects of the extract of Sanchen powder (ESCP) combined with vancomycin on methicillin-resistant Staphylococcus aureus (MRSA) planktonic cells, biofilms, and virulence factors. Methods: The herbs in Sanchen powder (SCP) were extracted separately with 50% ethanol. Then, the content of hydroxysafflower yellow A (HSYA) and cholic acid in the extract of artificial cattle bezoar (artificial Calculus bovis) and safflower (Carthamus tinctorius L.) was measured by ultraviolet visible-vis spectrophotometry and high-performance liquid chromatography. The effects of ESCP combined with vancomycin on MRSA by observing its biofilm viability were assessed using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium5-carboxanilide reduction assay and scanning electron microscopy. Additionally, enzyme activity was measured by plasma coagulase test and DNase test. Results: The cholic acid content of the artificial C. bovis extract was 7.34 (0.81) mg/g, and the HSYA content of the C. tinctorius extract was 9.18 (0.09) mg/g. The minimal inhibitory concentrations of ESCP and vancomycin were 25.6 mg/mL and 2 μg/mL. The minimum bactericidal concentration of ESCP was 51.2 mg/mL. ESCP combined with vancomycin could inhibit the expression of coagulase and bacteria in mature biofilms. Neither ESCP nor vancomycin had a significant effect on DNase. Conclusion: This study is the first to show that ESCP combined with vancomycin inhibits coagulase and MRSA embedded in mature biofilms and that it represents a promising treatment for MRSA infection. |
| first_indexed | 2024-03-12T18:04:35Z |
| format | Article |
| id | doaj.art-9092d93fac1a4fdab8e5518caf37411b |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T18:04:35Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-9092d93fac1a4fdab8e5518caf37411b2023-08-02T09:31:22ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482022-04-0192181187Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureusJiajia Fan0Honghao Sun1Yuanyuan Liu2Xin Li3Huichao Wu4Xiaoqiao Ren5School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaCorresponding author.; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, ChinaObjective: To explore the effects of the extract of Sanchen powder (ESCP) combined with vancomycin on methicillin-resistant Staphylococcus aureus (MRSA) planktonic cells, biofilms, and virulence factors. Methods: The herbs in Sanchen powder (SCP) were extracted separately with 50% ethanol. Then, the content of hydroxysafflower yellow A (HSYA) and cholic acid in the extract of artificial cattle bezoar (artificial Calculus bovis) and safflower (Carthamus tinctorius L.) was measured by ultraviolet visible-vis spectrophotometry and high-performance liquid chromatography. The effects of ESCP combined with vancomycin on MRSA by observing its biofilm viability were assessed using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium5-carboxanilide reduction assay and scanning electron microscopy. Additionally, enzyme activity was measured by plasma coagulase test and DNase test. Results: The cholic acid content of the artificial C. bovis extract was 7.34 (0.81) mg/g, and the HSYA content of the C. tinctorius extract was 9.18 (0.09) mg/g. The minimal inhibitory concentrations of ESCP and vancomycin were 25.6 mg/mL and 2 μg/mL. The minimum bactericidal concentration of ESCP was 51.2 mg/mL. ESCP combined with vancomycin could inhibit the expression of coagulase and bacteria in mature biofilms. Neither ESCP nor vancomycin had a significant effect on DNase. Conclusion: This study is the first to show that ESCP combined with vancomycin inhibits coagulase and MRSA embedded in mature biofilms and that it represents a promising treatment for MRSA infection.http://www.sciencedirect.com/science/article/pii/S2095754822000229Tibetan medicineSanchen powderVancomycinMethicillin-resistant Staphylococcus aureusAntibacterial effectTraditional Chinese medicine |
| spellingShingle | Jiajia Fan Honghao Sun Yuanyuan Liu Xin Li Huichao Wu Xiaoqiao Ren Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus Journal of Traditional Chinese Medical Sciences Tibetan medicine Sanchen powder Vancomycin Methicillin-resistant Staphylococcus aureus Antibacterial effect Traditional Chinese medicine |
| title | Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus |
| title_full | Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus |
| title_fullStr | Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus |
| title_short | Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus |
| title_sort | sanchen powder extract combined with vancomycin against methicillin resistant staphylococcus aureus |
| topic | Tibetan medicine Sanchen powder Vancomycin Methicillin-resistant Staphylococcus aureus Antibacterial effect Traditional Chinese medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2095754822000229 |
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