Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics
In the present trial, 160 heavy-size male broiler chickens were allocated to 4 dietary treatments (control feed [C] and 5, 10 and 15% TM meal inclusion, respectively, with 5 replicate pens/treatment and 8 birds/pen) to evaluate the influence of TM meal on intestinal microbiota and mucin composition....
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1909 |
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| author | Ilaria Biasato Ilario Ferrocino Elena Grego Sihem Dabbou Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone |
| author_facet | Ilaria Biasato Ilario Ferrocino Elena Grego Sihem Dabbou Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone |
| author_sort | Ilaria Biasato |
| collection | DOAJ |
| description | In the present trial, 160 heavy-size male broiler chickens were allocated to 4 dietary treatments (control feed [C] and 5, 10 and 15% TM meal inclusion, respectively, with 5 replicate pens/treatment and 8 birds/pen) to evaluate the influence of TM meal on intestinal microbiota and mucin composition. The broiler chickens fed TM-based diets showed higher beta diversity of their cecal microbiota in comparison with the C birds (<i>p</i> < 0.001). A significant decrease of the relative abundance of Firmicutes phylum and lower Firmicutes:Bacteroidetes ratios (False Discovery Rate [FDR] < 0.05) were also identified in TM15 broiler chickens when compared to the C group. Furthermore, the TM birds showed decreased relative abundance of <i>Clostridium, Coprococcus,</i> L-<i>Ruminococcus and Ruminococcus</i> genera (FDR < 0.05). In relation to the gut mucin composition, higher mucin staining intensity was detected in the intestinal crypts of TM5 birds in comparison with the other TM groups (<i>p</i> < 0.05). In conclusion, dietary TM meal inclusion negatively influenced the cecal microbiota of heavy-size broiler chickens in terms of partial alteration of the physiological microbial population and reduction of the potential beneficial bacteria (with slightly more pronounced effects when testing the 10–15% inclusion levels). |
| first_indexed | 2024-03-10T15:31:40Z |
| format | Article |
| id | doaj.art-909370a71f3b479693020eb4fdd75108 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T15:31:40Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-909370a71f3b479693020eb4fdd751082023-11-20T17:35:15ZengMDPI AGAnimals2076-26152020-10-011010190910.3390/ani10101909Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin DynamicsIlaria Biasato0Ilario Ferrocino1Elena Grego2Sihem Dabbou3Francesco Gai4Laura Gasco5Luca Cocolin6Maria Teresa Capucchio7Achille Schiavone8Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyCenter Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, ItalyInstitute of Science of Food Production, National Research Council, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyDepartment of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), ItalyIn the present trial, 160 heavy-size male broiler chickens were allocated to 4 dietary treatments (control feed [C] and 5, 10 and 15% TM meal inclusion, respectively, with 5 replicate pens/treatment and 8 birds/pen) to evaluate the influence of TM meal on intestinal microbiota and mucin composition. The broiler chickens fed TM-based diets showed higher beta diversity of their cecal microbiota in comparison with the C birds (<i>p</i> < 0.001). A significant decrease of the relative abundance of Firmicutes phylum and lower Firmicutes:Bacteroidetes ratios (False Discovery Rate [FDR] < 0.05) were also identified in TM15 broiler chickens when compared to the C group. Furthermore, the TM birds showed decreased relative abundance of <i>Clostridium, Coprococcus,</i> L-<i>Ruminococcus and Ruminococcus</i> genera (FDR < 0.05). In relation to the gut mucin composition, higher mucin staining intensity was detected in the intestinal crypts of TM5 birds in comparison with the other TM groups (<i>p</i> < 0.05). In conclusion, dietary TM meal inclusion negatively influenced the cecal microbiota of heavy-size broiler chickens in terms of partial alteration of the physiological microbial population and reduction of the potential beneficial bacteria (with slightly more pronounced effects when testing the 10–15% inclusion levels).https://www.mdpi.com/2076-2615/10/10/1909insect mealmicrobiotamucinbroiler chicken |
| spellingShingle | Ilaria Biasato Ilario Ferrocino Elena Grego Sihem Dabbou Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics Animals insect meal microbiota mucin broiler chicken |
| title | Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics |
| title_full | Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics |
| title_fullStr | Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics |
| title_full_unstemmed | Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics |
| title_short | Yellow Mealworm Inclusion in Diets for Heavy-Size Broiler Chickens: Implications for Intestinal Microbiota and Mucin Dynamics |
| title_sort | yellow mealworm inclusion in diets for heavy size broiler chickens implications for intestinal microbiota and mucin dynamics |
| topic | insect meal microbiota mucin broiler chicken |
| url | https://www.mdpi.com/2076-2615/10/10/1909 |
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