Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1
Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset o...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00825/full |
| _version_ | 1811197969491296256 |
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| author | Daniel Perez-Zsolt Daniel Perez-Zsolt Jon Cantero-Pérez Jon Cantero-Pérez Itziar Erkizia Susana Benet Susana Benet Maria Pino Carla Serra-Peinado Alba Hernández-Gallego Alba Hernández-Gallego Josep Castellví Josep Castellví Gustavo Tapia Gustavo Tapia Gustavo Tapia Vicent Arnau-Saz Vicent Arnau-Saz Julio Garrido Antoni Tarrats Maria J. Buzón Javier Martinez-Picado Javier Martinez-Picado Javier Martinez-Picado Nuria Izquierdo-Useros Nuria Izquierdo-Useros Meritxell Genescà Meritxell Genescà |
| author_facet | Daniel Perez-Zsolt Daniel Perez-Zsolt Jon Cantero-Pérez Jon Cantero-Pérez Itziar Erkizia Susana Benet Susana Benet Maria Pino Carla Serra-Peinado Alba Hernández-Gallego Alba Hernández-Gallego Josep Castellví Josep Castellví Gustavo Tapia Gustavo Tapia Gustavo Tapia Vicent Arnau-Saz Vicent Arnau-Saz Julio Garrido Antoni Tarrats Maria J. Buzón Javier Martinez-Picado Javier Martinez-Picado Javier Martinez-Picado Nuria Izquierdo-Useros Nuria Izquierdo-Useros Meritxell Genescà Meritxell Genescà |
| author_sort | Daniel Perez-Zsolt |
| collection | DOAJ |
| description | Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies. |
| first_indexed | 2024-04-12T01:23:21Z |
| format | Article |
| id | doaj.art-90950858fdf74ccbb893ee3f80d7fed6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T01:23:21Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-90950858fdf74ccbb893ee3f80d7fed62022-12-22T03:53:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-04-011010.3389/fimmu.2019.00825447514Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1Daniel Perez-Zsolt0Daniel Perez-Zsolt1Jon Cantero-Pérez2Jon Cantero-Pérez3Itziar Erkizia4Susana Benet5Susana Benet6Maria Pino7Carla Serra-Peinado8Alba Hernández-Gallego9Alba Hernández-Gallego10Josep Castellví11Josep Castellví12Gustavo Tapia13Gustavo Tapia14Gustavo Tapia15Vicent Arnau-Saz16Vicent Arnau-Saz17Julio Garrido18Antoni Tarrats19Maria J. Buzón20Javier Martinez-Picado21Javier Martinez-Picado22Javier Martinez-Picado23Nuria Izquierdo-Useros24Nuria Izquierdo-Useros25Meritxell Genescà26Meritxell Genescà27IrsiCaixa AIDS Research Institute, Badalona, SpainDepartment of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Infectious Diseases, Vall d'Hebron Institut de Recerca, Barcelona, SpainInstitut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, SpainIrsiCaixa AIDS Research Institute, Badalona, SpainIrsiCaixa AIDS Research Institute, Badalona, SpainDepartment of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Barcelona, SpainIrsiCaixa AIDS Research Institute, Badalona, SpainDepartment of Infectious Diseases, Vall d'Hebron Institut de Recerca, Barcelona, SpainInstitut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, SpainPathology Department, Hospital Universitari Germans Trias i Pujol, Badalona, SpainPathology Department, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Morphological Sciences, Universitat Autònoma de Barcelona, Barcelona, SpainInstitut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, SpainPathology Department, Hospital Universitari Germans Trias i Pujol, Badalona, SpainDepartment of Morphological Sciences, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Infectious Diseases, Vall d'Hebron Institut de Recerca, Barcelona, SpainHospital Municipal de Badalona, Badalona, SpainDepartment of Obstetrics and Gynecology, Hospital Universitari Germans Trias i Pujol, Badalona, SpainDepartment of Infectious Diseases, Vall d'Hebron Institut de Recerca, Barcelona, SpainIrsiCaixa AIDS Research Institute, Badalona, Spain0University of Vic-Central University of Catalonia (UVic-UCC), Vic, Spain1Institució Catalana de Recerca i Estudis Avançats, Barcelona, SpainIrsiCaixa AIDS Research Institute, Badalona, SpainInstitut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, SpainDepartment of Infectious Diseases, Vall d'Hebron Institut de Recerca, Barcelona, SpainInstitut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, SpainAntigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. Yet, the molecular mechanism at the cervical mucosa underlying this viral transmission pathway remains unknown. Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies.https://www.frontiersin.org/article/10.3389/fimmu.2019.00825/fullcervixSiglec-1HIV-1trans-infectionmyeloid cells |
| spellingShingle | Daniel Perez-Zsolt Daniel Perez-Zsolt Jon Cantero-Pérez Jon Cantero-Pérez Itziar Erkizia Susana Benet Susana Benet Maria Pino Carla Serra-Peinado Alba Hernández-Gallego Alba Hernández-Gallego Josep Castellví Josep Castellví Gustavo Tapia Gustavo Tapia Gustavo Tapia Vicent Arnau-Saz Vicent Arnau-Saz Julio Garrido Antoni Tarrats Maria J. Buzón Javier Martinez-Picado Javier Martinez-Picado Javier Martinez-Picado Nuria Izquierdo-Useros Nuria Izquierdo-Useros Meritxell Genescà Meritxell Genescà Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 Frontiers in Immunology cervix Siglec-1 HIV-1 trans-infection myeloid cells |
| title | Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
| title_full | Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
| title_fullStr | Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
| title_full_unstemmed | Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
| title_short | Dendritic Cells From the Cervical Mucosa Capture and Transfer HIV-1 via Siglec-1 |
| title_sort | dendritic cells from the cervical mucosa capture and transfer hiv 1 via siglec 1 |
| topic | cervix Siglec-1 HIV-1 trans-infection myeloid cells |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00825/full |
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