Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources
IntroductionIn marine ecosystems, microbial communities are important drivers of material circulation and energy flow. The complex interactions between phytoplankton and bacterial communities constitute one of the most crucial ecological relationships in the marine environment. Inorganic nitrogen ca...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1086166/full |
| _version_ | 1797895416545542144 |
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| author | Xiaoxue Wei Feng Shi Zixi Chen Jianfeng Feng Jianfeng Feng Lin Zhu |
| author_facet | Xiaoxue Wei Feng Shi Zixi Chen Jianfeng Feng Jianfeng Feng Lin Zhu |
| author_sort | Xiaoxue Wei |
| collection | DOAJ |
| description | IntroductionIn marine ecosystems, microbial communities are important drivers of material circulation and energy flow. The complex interactions between phytoplankton and bacterial communities constitute one of the most crucial ecological relationships in the marine environment. Inorganic nitrogen can affect the type of relationship between algae and bacteria. However, the quantitative relationship between the bacterial communities, inorganic nitrogen, and phytoplankton remains unclear.MethodsUnder laboratory conditions, we altered the forms (nitrate and ammonium) and amounts of nitrogen sources to study the dynamics of bacterial biomass, diversity, and community structure in the phycosphere of the marine model species Phaeodactylum tricornutum. The bacterial community structure during P. tricornutum growth was analyzed using Illumina HiSeq sequencing of 16S rDNA amplicons.ResultsThe results indicated that inorganic nitrogen concentration was the main factor promoting P. tricornutum biomass growth. The change in the algal biomass would significantly increase the phycosphere bacterial biomass. The bacterial biomass in the algal-bacteria co-culture system was 1.5 ~ 5 times that of the conditional control groups without microalgae under the same culture conditions. The variation of P. tricornutum biomass also affected the bacterial communities in the phycosphere. When P. tricornutum was in the exponential phase (96 ~ 192 h), the bacterial community structure differed between the high- and low-concentration groups. The difference in the bacterial communities over time in the high-concentration groups was more prominent than in the low-concentration groups. Under high-concentration groups (HA and HN), the relative abundance of Marivita and Marinobacter, engaged in the transformation of aquatic inorganic nitrogen, gradually decreased with time. However, the relative abundance of Oceanicaulis, closely related to algal growth, gradually increased with time.DiscussionThe above phenomena might be related to the change in P. tricornutum biomass. Our results explain when and how the phycosphere bacterial communities responded to algal biomass variations. The study provides a foundation for the quantitative relationship among nutrients, microalgae, and bacteria in this system. |
| first_indexed | 2024-04-10T07:26:32Z |
| format | Article |
| id | doaj.art-9095da9e05eb4ddf9bcdcc3387ffb5c0 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T07:26:32Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-9095da9e05eb4ddf9bcdcc3387ffb5c02023-02-24T06:59:41ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-02-011010.3389/fmars.2023.10861661086166Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sourcesXiaoxue Wei0Feng Shi1Zixi Chen2Jianfeng Feng3Jianfeng Feng4Lin Zhu5College of Environmental Science and Engineering, Nankai University, Tianjin, ChinaDepartment of Government and Innovation Performance Evaluation, National Center for Science & Technology Evaluation, Beijing, ChinaCollege of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCollege of Environmental Science and Engineering, Nankai University, Tianjin, ChinaTianjin Key Laboratory of Environmental Treatment Technology for Complex Trans-Media Pollution, Nankai University, Tianjin, ChinaCollege of Environmental Science and Engineering, Nankai University, Tianjin, ChinaIntroductionIn marine ecosystems, microbial communities are important drivers of material circulation and energy flow. The complex interactions between phytoplankton and bacterial communities constitute one of the most crucial ecological relationships in the marine environment. Inorganic nitrogen can affect the type of relationship between algae and bacteria. However, the quantitative relationship between the bacterial communities, inorganic nitrogen, and phytoplankton remains unclear.MethodsUnder laboratory conditions, we altered the forms (nitrate and ammonium) and amounts of nitrogen sources to study the dynamics of bacterial biomass, diversity, and community structure in the phycosphere of the marine model species Phaeodactylum tricornutum. The bacterial community structure during P. tricornutum growth was analyzed using Illumina HiSeq sequencing of 16S rDNA amplicons.ResultsThe results indicated that inorganic nitrogen concentration was the main factor promoting P. tricornutum biomass growth. The change in the algal biomass would significantly increase the phycosphere bacterial biomass. The bacterial biomass in the algal-bacteria co-culture system was 1.5 ~ 5 times that of the conditional control groups without microalgae under the same culture conditions. The variation of P. tricornutum biomass also affected the bacterial communities in the phycosphere. When P. tricornutum was in the exponential phase (96 ~ 192 h), the bacterial community structure differed between the high- and low-concentration groups. The difference in the bacterial communities over time in the high-concentration groups was more prominent than in the low-concentration groups. Under high-concentration groups (HA and HN), the relative abundance of Marivita and Marinobacter, engaged in the transformation of aquatic inorganic nitrogen, gradually decreased with time. However, the relative abundance of Oceanicaulis, closely related to algal growth, gradually increased with time.DiscussionThe above phenomena might be related to the change in P. tricornutum biomass. Our results explain when and how the phycosphere bacterial communities responded to algal biomass variations. The study provides a foundation for the quantitative relationship among nutrients, microalgae, and bacteria in this system.https://www.frontiersin.org/articles/10.3389/fmars.2023.1086166/fullbacterial community structureammoniumnitratePhaeodactylum tricornutummarine |
| spellingShingle | Xiaoxue Wei Feng Shi Zixi Chen Jianfeng Feng Jianfeng Feng Lin Zhu Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources Frontiers in Marine Science bacterial community structure ammonium nitrate Phaeodactylum tricornutum marine |
| title | Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources |
| title_full | Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources |
| title_fullStr | Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources |
| title_full_unstemmed | Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources |
| title_short | Response of bacterial communities (Marivita, Marinobacter, and Oceanicaulis) in the phycosphere to the growth of Phaeodactylum tricornutum in different inorganic nitrogen sources |
| title_sort | response of bacterial communities marivita marinobacter and oceanicaulis in the phycosphere to the growth of phaeodactylum tricornutum in different inorganic nitrogen sources |
| topic | bacterial community structure ammonium nitrate Phaeodactylum tricornutum marine |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1086166/full |
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