Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat
Dendritic cell activating receptor-1 (DCAR1) is a cell-surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody against rat DCAR1, and used this to characterize receptor expression and function. Rat DCAR1 was expressed on minor sub...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01060/full |
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| author | Michael R. Daws Britt Nakken Ana Lobato-Pascual Régis Josien Régis Josien Erik Dissen Sigbjørn Fossum |
| author_facet | Michael R. Daws Britt Nakken Ana Lobato-Pascual Régis Josien Régis Josien Erik Dissen Sigbjørn Fossum |
| author_sort | Michael R. Daws |
| collection | DOAJ |
| description | Dendritic cell activating receptor-1 (DCAR1) is a cell-surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody against rat DCAR1, and used this to characterize receptor expression and function. Rat DCAR1 was expressed on minor subsets of myeloid cells in lymphoid tissue, but was uniformly expressed at a high level by eosinophils, and at a low level by neutrophils. It was expressed by eosinophils in the peritoneal cavity and the lamina propria of the gut, and by subsets of macrophages or dendritic cells at these sites. Polarization of peritoneal macrophages showed that DCAR1 expression was absent on M1 macrophages, and increased on M2 macrophages. DCAR1 could be expressed as a homodimer and its associated with the activating adaptor protein FcεRIγ. This association allowed efficient phagocytosis of antibody-coated beads. Additionally, cross-linking of DCAR1 on the surface of rat eosinophils lead to production of reactive oxygen species. These data show that DCAR1 is an activating receptor. Its expression on M2 macrophages and eosinophils suggests that it may play a role in the immune response to parasites. |
| first_indexed | 2024-04-12T19:14:51Z |
| format | Article |
| id | doaj.art-909677a2645e4962b109cbfcc056486c |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T19:14:51Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-909677a2645e4962b109cbfcc056486c2022-12-22T03:19:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-05-011010.3389/fimmu.2019.01060438602Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the RatMichael R. Daws0Britt Nakken1Ana Lobato-Pascual2Régis Josien3Régis Josien4Erik Dissen5Sigbjørn Fossum6Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceLaboratoire d'Immunologie, CHU Nantes, Nantes, FranceDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDendritic cell activating receptor-1 (DCAR1) is a cell-surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody against rat DCAR1, and used this to characterize receptor expression and function. Rat DCAR1 was expressed on minor subsets of myeloid cells in lymphoid tissue, but was uniformly expressed at a high level by eosinophils, and at a low level by neutrophils. It was expressed by eosinophils in the peritoneal cavity and the lamina propria of the gut, and by subsets of macrophages or dendritic cells at these sites. Polarization of peritoneal macrophages showed that DCAR1 expression was absent on M1 macrophages, and increased on M2 macrophages. DCAR1 could be expressed as a homodimer and its associated with the activating adaptor protein FcεRIγ. This association allowed efficient phagocytosis of antibody-coated beads. Additionally, cross-linking of DCAR1 on the surface of rat eosinophils lead to production of reactive oxygen species. These data show that DCAR1 is an activating receptor. Its expression on M2 macrophages and eosinophils suggests that it may play a role in the immune response to parasites.https://www.frontiersin.org/article/10.3389/fimmu.2019.01060/fullmacrophagegranulocytedendritic cellC-type lectinreceptor |
| spellingShingle | Michael R. Daws Britt Nakken Ana Lobato-Pascual Régis Josien Régis Josien Erik Dissen Sigbjørn Fossum Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat Frontiers in Immunology macrophage granulocyte dendritic cell C-type lectin receptor |
| title | Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat |
| title_full | Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat |
| title_fullStr | Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat |
| title_full_unstemmed | Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat |
| title_short | Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat |
| title_sort | dendritic cell activating receptor 1 dcar1 associates with fcεriγ and is expressed by myeloid cell subsets in the rat |
| topic | macrophage granulocyte dendritic cell C-type lectin receptor |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01060/full |
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