Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells
Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/4/381 |
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| author | Giulia Orlandi Elia Bari Laura Catenacci Milena Sorrenti Lorena Segale Silvio Faragò Marzio Sorlini Carla Renata Arciola Maria Luisa Torre Sara Perteghella |
| author_facet | Giulia Orlandi Elia Bari Laura Catenacci Milena Sorrenti Lorena Segale Silvio Faragò Marzio Sorlini Carla Renata Arciola Maria Luisa Torre Sara Perteghella |
| author_sort | Giulia Orlandi |
| collection | DOAJ |
| description | Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs. |
| first_indexed | 2024-03-10T20:20:27Z |
| format | Article |
| id | doaj.art-909795cefc304e639f2ad4bc85b6040c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:20:27Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-909795cefc304e639f2ad4bc85b6040c2023-11-19T22:15:02ZengMDPI AGPharmaceutics1999-49232020-04-0112438110.3390/pharmaceutics12040381Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal CellsGiulia Orlandi0Elia Bari1Laura Catenacci2Milena Sorrenti3Lorena Segale4Silvio Faragò5Marzio Sorlini6Carla Renata Arciola7Maria Luisa Torre8Sara Perteghella9Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Largo Donegani 2/3, 28100 Novara, ItalySilk Division, Innovhub, Stazioni Sperimentali per l’Industria, 20133 Milan, ItalyInnovative Technologies Department, SUPSI, University of Applied Sciences and Arts of Southern Switzerland, Via Pobiette 11, 6928 Manno, SwitzerlandLaboratorio di Patologia delle Infezioni Associate all’Impianto, IRCCS Istituto ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyMesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs.https://www.mdpi.com/1999-4923/12/4/381silk-sericin nanoparticlesproanthocyanidinsquercetinepigallocatechin gallatetissue regenerationmesenchymal stem/stromal cells |
| spellingShingle | Giulia Orlandi Elia Bari Laura Catenacci Milena Sorrenti Lorena Segale Silvio Faragò Marzio Sorlini Carla Renata Arciola Maria Luisa Torre Sara Perteghella Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells Pharmaceutics silk-sericin nanoparticles proanthocyanidins quercetin epigallocatechin gallate tissue regeneration mesenchymal stem/stromal cells |
| title | Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells |
| title_full | Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells |
| title_fullStr | Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells |
| title_full_unstemmed | Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells |
| title_short | Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells |
| title_sort | polyphenols loaded sericin self assembling nanoparticles a slow release for regeneration by tissue resident mesenchymal stem stromal cells |
| topic | silk-sericin nanoparticles proanthocyanidins quercetin epigallocatechin gallate tissue regeneration mesenchymal stem/stromal cells |
| url | https://www.mdpi.com/1999-4923/12/4/381 |
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