Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells
Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed a correlation between exposure to IR and the development of eye lens cataracts. Cataracts remain the leading cause of blindness around the world. The plasma membranes of lens fibre cells are one of the m...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Advances in Redox Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667137922000297 |
| _version_ | 1797871522923151360 |
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| author | Alice Uwineza Ian Cummins Miguel Jarrin Alexia A. Kalligeraki Stephen Barnard Marco Mol Genny Degani Alessandra A. Altomare Giancarlo Aldini An Schreurs Detlef Balschun Elizabeth A. Ainsbury Irundika HK Dias Roy A. Quinlan |
| author_facet | Alice Uwineza Ian Cummins Miguel Jarrin Alexia A. Kalligeraki Stephen Barnard Marco Mol Genny Degani Alessandra A. Altomare Giancarlo Aldini An Schreurs Detlef Balschun Elizabeth A. Ainsbury Irundika HK Dias Roy A. Quinlan |
| author_sort | Alice Uwineza |
| collection | DOAJ |
| description | Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed a correlation between exposure to IR and the development of eye lens cataracts. Cataracts remain the leading cause of blindness around the world. The plasma membranes of lens fibre cells are one of the most cholesterolrich membranes in the human body, forming lipid rafts and contributing to the biophysical properties of lens fibre plasma membrane. Liquid chromatography followed by mass spectrometry was used to analyse bovine eye lens lipid membrane fractions after exposure to 5 and 50 Gy and eye lenses taken from wholebody 2 Gy-irradiated mice. Although cholesterol levels do not change significantly, IR dose-dependant formation of the oxysterols 7β-hydroxycholesterol, 7-ketocholesterol and 5, 6-epoxycholesterol in bovine lens nucleus membrane extracts was observed. Whole-body X-ray exposure (2 Gy) of 12-week old mice resulted in an increase in 7β-hydroxycholesterol and 7-ketocholesterol in their eye lenses. Their increase regressed over 24 h in the living lens cortex after IR exposure. This study also demonstrated that the IR-induced fold increase in oxysterols was greater in the mouse lens cortex than the nucleus. Further work is required to elucidate the mechanistic link(s) between oxysterols and IR-induced cataract, but these data evidence for the first time that IR exposure of mice results in oxysterol formation in their eye lenses. |
| first_indexed | 2024-04-10T00:44:07Z |
| format | Article |
| id | doaj.art-909a757273cb4520b74c0f1bdaf82b5f |
| institution | Directory Open Access Journal |
| issn | 2667-1379 |
| language | English |
| last_indexed | 2024-04-10T00:44:07Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Redox Research |
| spelling | doaj.art-909a757273cb4520b74c0f1bdaf82b5f2023-03-14T04:09:11ZengElsevierAdvances in Redox Research2667-13792023-04-017100057Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cellsAlice Uwineza0Ian Cummins1Miguel Jarrin2Alexia A. Kalligeraki3Stephen Barnard4Marco Mol5Genny Degani6Alessandra A. Altomare7Giancarlo Aldini8An Schreurs9Detlef Balschun10Elizabeth A. Ainsbury11Irundika HK Dias12Roy A. Quinlan13Department of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United Kingdom; Biophysical Sciences Institute, University of Durham, South Road, Durham D1 3LE, United KingdomDepartment of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United KingdomDepartment of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United Kingdom; Biophysical Sciences Institute, University of Durham, South Road, Durham D1 3LE, United KingdomDepartment of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United Kingdom; Biophysical Sciences Institute, University of Durham, South Road, Durham D1 3LE, United KingdomDepartment of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United Kingdom; Biophysical Sciences Institute, University of Durham, South Road, Durham D1 3LE, United Kingdom; UK Health Security Agency, Cytogenetics and Pathology Group, Centre for Radiation, Chemical and Environmental Hazards Division, Chilton, Oxon OX11 0RQ, Didcot, United KingdomDepartment of Pharmaceutical Sciences, Via Mangiagalli 25, Milano 20133, ItalyDepartment of Biosciences, Via Celoria 26, Milano 20133, ItalyDepartment of Pharmaceutical Sciences, Via Mangiagalli 25, Milano 20133, ItalyDepartment of Pharmaceutical Sciences, Via Mangiagalli 25, Milano 20133, ItalyBrain & Cognition, Leuven Brain Institute, KU Leuven, Leuven, BelgiumBrain & Cognition, Leuven Brain Institute, KU Leuven, Leuven, BelgiumUK Health Security Agency, Cytogenetics and Pathology Group, Centre for Radiation, Chemical and Environmental Hazards Division, Chilton, Oxon OX11 0RQ, Didcot, United KingdomAston Medical School, Aston University, B4 7ET, Birmingham, United Kingdom; Corresponding authors.Department of Biosciences, University of Durham, Upper Mountjoy Science Site, South Road, Durham DH1 3LE, United Kingdom; Biophysical Sciences Institute, University of Durham, South Road, Durham D1 3LE, United Kingdom; Department of Biological Structure, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, United States; Corresponding authors.Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed a correlation between exposure to IR and the development of eye lens cataracts. Cataracts remain the leading cause of blindness around the world. The plasma membranes of lens fibre cells are one of the most cholesterolrich membranes in the human body, forming lipid rafts and contributing to the biophysical properties of lens fibre plasma membrane. Liquid chromatography followed by mass spectrometry was used to analyse bovine eye lens lipid membrane fractions after exposure to 5 and 50 Gy and eye lenses taken from wholebody 2 Gy-irradiated mice. Although cholesterol levels do not change significantly, IR dose-dependant formation of the oxysterols 7β-hydroxycholesterol, 7-ketocholesterol and 5, 6-epoxycholesterol in bovine lens nucleus membrane extracts was observed. Whole-body X-ray exposure (2 Gy) of 12-week old mice resulted in an increase in 7β-hydroxycholesterol and 7-ketocholesterol in their eye lenses. Their increase regressed over 24 h in the living lens cortex after IR exposure. This study also demonstrated that the IR-induced fold increase in oxysterols was greater in the mouse lens cortex than the nucleus. Further work is required to elucidate the mechanistic link(s) between oxysterols and IR-induced cataract, but these data evidence for the first time that IR exposure of mice results in oxysterol formation in their eye lenses.http://www.sciencedirect.com/science/article/pii/S2667137922000297Eye lensAge-related cataractCholesterolLipid raftsX-raysIonising radiation |
| spellingShingle | Alice Uwineza Ian Cummins Miguel Jarrin Alexia A. Kalligeraki Stephen Barnard Marco Mol Genny Degani Alessandra A. Altomare Giancarlo Aldini An Schreurs Detlef Balschun Elizabeth A. Ainsbury Irundika HK Dias Roy A. Quinlan Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells Advances in Redox Research Eye lens Age-related cataract Cholesterol Lipid rafts X-rays Ionising radiation |
| title | Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells |
| title_full | Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells |
| title_fullStr | Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells |
| title_full_unstemmed | Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells |
| title_short | Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells |
| title_sort | identification and quantification of ionising radiation induced oxysterol formation in membranes of lens fibre cells |
| topic | Eye lens Age-related cataract Cholesterol Lipid rafts X-rays Ionising radiation |
| url | http://www.sciencedirect.com/science/article/pii/S2667137922000297 |
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