BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing

BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing

Abstract Objective We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing. Results BRAF V600 mutations of the V600E type were...

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Bibliographic Details
Main Authors: Hanggoro Tri Rinonce, Rovi Panji Mustiko Aji, Ni’mah Hayati, Maria Fransiska Pudjohartono, Bidari Kameswari, Sumadi Lukman Anwar, Irianiwati
Format: Article
Language:English
Published: BMC 2020-03-01
Series:BMC Research Notes
Subjects:
Melanoma
Skin cancer
BRAF
Pyrosequencing
Indonesia
Online Access:http://link.springer.com/article/10.1186/s13104-020-05000-w
Description
Summary:Abstract Objective We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing. Results BRAF V600 mutations of the V600E type were found in 21 (53.85%) specimens. The variant allele frequencies (VAFs) ranged from 5.07 to 94.70%, averaging 29.05%. However, most cases had low VAFs, with 13 (61.9%) specimens below 20% and 4 (19.05%) below 10%.
ISSN:1756-0500

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