Fluorescence Polarization-Based Bioassays: New Horizons
Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the prepar...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/20/24/7132 |
| _version_ | 1797544894246420480 |
|---|---|
| author | Olga D. Hendrickson Nadezhda A. Taranova Anatoly V. Zherdev Boris B. Dzantiev Sergei A. Eremin |
| author_facet | Olga D. Hendrickson Nadezhda A. Taranova Anatoly V. Zherdev Boris B. Dzantiev Sergei A. Eremin |
| author_sort | Olga D. Hendrickson |
| collection | DOAJ |
| description | Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples. For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on differences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex. However, the results of recent studies demonstrate the efficacy of fluorescence polarization as a detected signal in many bioanalytical methods. This review summarizes and comparatively characterizes these developments. It considers the integration of fluorescence polarization with the use of alternative receptor molecules and various fluorophores; different schemes for the formation of detectable complexes and the amplification of the signals generated by them. New techniques for the detection of metal ions, nucleic acids, and enzymatic reactions based on fluorescence polarization are also considered. |
| first_indexed | 2024-03-10T14:07:55Z |
| format | Article |
| id | doaj.art-909fcd5762794083a49c0db9f46180d1 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T14:07:55Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-909fcd5762794083a49c0db9f46180d12023-11-21T00:32:02ZengMDPI AGSensors1424-82202020-12-012024713210.3390/s20247132Fluorescence Polarization-Based Bioassays: New HorizonsOlga D. Hendrickson0Nadezhda A. Taranova1Anatoly V. Zherdev2Boris B. Dzantiev3Sergei A. Eremin4A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaFluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples. For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on differences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex. However, the results of recent studies demonstrate the efficacy of fluorescence polarization as a detected signal in many bioanalytical methods. This review summarizes and comparatively characterizes these developments. It considers the integration of fluorescence polarization with the use of alternative receptor molecules and various fluorophores; different schemes for the formation of detectable complexes and the amplification of the signals generated by them. New techniques for the detection of metal ions, nucleic acids, and enzymatic reactions based on fluorescence polarization are also considered.https://www.mdpi.com/1424-8220/20/24/7132fluorescence polarizationimmunoassayrotation of moleculesbioreceptorsantibodiesaptamers |
| spellingShingle | Olga D. Hendrickson Nadezhda A. Taranova Anatoly V. Zherdev Boris B. Dzantiev Sergei A. Eremin Fluorescence Polarization-Based Bioassays: New Horizons Sensors fluorescence polarization immunoassay rotation of molecules bioreceptors antibodies aptamers |
| title | Fluorescence Polarization-Based Bioassays: New Horizons |
| title_full | Fluorescence Polarization-Based Bioassays: New Horizons |
| title_fullStr | Fluorescence Polarization-Based Bioassays: New Horizons |
| title_full_unstemmed | Fluorescence Polarization-Based Bioassays: New Horizons |
| title_short | Fluorescence Polarization-Based Bioassays: New Horizons |
| title_sort | fluorescence polarization based bioassays new horizons |
| topic | fluorescence polarization immunoassay rotation of molecules bioreceptors antibodies aptamers |
| url | https://www.mdpi.com/1424-8220/20/24/7132 |
| work_keys_str_mv | AT olgadhendrickson fluorescencepolarizationbasedbioassaysnewhorizons AT nadezhdaataranova fluorescencepolarizationbasedbioassaysnewhorizons AT anatolyvzherdev fluorescencepolarizationbasedbioassaysnewhorizons AT borisbdzantiev fluorescencepolarizationbasedbioassaysnewhorizons AT sergeiaeremin fluorescencepolarizationbasedbioassaysnewhorizons |