Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products
The artificial production of <i>Ophiocordyceps</i> <i>sinensis</i> mycelia and fruiting bodies and the Chinese cordyceps has been established. However, the volatile components from these <i>O. sinensis</i> products are not fully identified. An efficient, convenien...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/7/1603 |
| _version_ | 1797571823069560832 |
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| author | Xuehong Qiu Li Cao Richou Han |
| author_facet | Xuehong Qiu Li Cao Richou Han |
| author_sort | Xuehong Qiu |
| collection | DOAJ |
| description | The artificial production of <i>Ophiocordyceps</i> <i>sinensis</i> mycelia and fruiting bodies and the Chinese cordyceps has been established. However, the volatile components from these <i>O. sinensis</i> products are not fully identified. An efficient, convenient, and widely used approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography and quadrupole time-of-flight mass spectrometry (GC×GC-QTOFMS) was developed for the extraction and the analysis of volatile compounds from three categories of 16 products, including <i>O. sinensis</i> fungus, <i>Thitarodes</i> hosts of <i>O. sinensis</i>, and the Chinese cordyceps. A total of 120 volatile components including 36 alkanes, 25 terpenes, 17 aromatic hydrocarbons, 10 ketones, 5 olefines, 5 alcohols, 3 phenols, and 19 other compounds were identified. The contents of these components varied greatly among the products but alkanes, especially 2,5,6-trimethyldecane, 2,3-dimethylundecane and 2,2,4,4-tetramethyloctane, are the dominant compounds in general. Three categories of volatile compounds were confirmed by partial least squares-discriminant analysis (PLS-DA). This study provided an ideal method for characterizing and distinguishing different <i>O. sinensis</i> and insect hosts-based products. |
| first_indexed | 2024-03-10T20:47:02Z |
| format | Article |
| id | doaj.art-90a04a6db0e04db4a5a498bfac660bce |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:47:02Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-90a04a6db0e04db4a5a498bfac660bce2023-11-19T20:16:30ZengMDPI AGMolecules1420-30492020-03-01257160310.3390/molecules25071603Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host ProductsXuehong Qiu0Li Cao1Richou Han2Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, Guangdong, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, Guangdong, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, Guangdong, ChinaThe artificial production of <i>Ophiocordyceps</i> <i>sinensis</i> mycelia and fruiting bodies and the Chinese cordyceps has been established. However, the volatile components from these <i>O. sinensis</i> products are not fully identified. An efficient, convenient, and widely used approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography and quadrupole time-of-flight mass spectrometry (GC×GC-QTOFMS) was developed for the extraction and the analysis of volatile compounds from three categories of 16 products, including <i>O. sinensis</i> fungus, <i>Thitarodes</i> hosts of <i>O. sinensis</i>, and the Chinese cordyceps. A total of 120 volatile components including 36 alkanes, 25 terpenes, 17 aromatic hydrocarbons, 10 ketones, 5 olefines, 5 alcohols, 3 phenols, and 19 other compounds were identified. The contents of these components varied greatly among the products but alkanes, especially 2,5,6-trimethyldecane, 2,3-dimethylundecane and 2,2,4,4-tetramethyloctane, are the dominant compounds in general. Three categories of volatile compounds were confirmed by partial least squares-discriminant analysis (PLS-DA). This study provided an ideal method for characterizing and distinguishing different <i>O. sinensis</i> and insect hosts-based products.https://www.mdpi.com/1420-3049/25/7/1603<i>Ophiocordyceps sinensis</i><i>Thitarodes</i> hostsChinese cordycepsvolatile compoundsHS-SPME GC×GC-QTOFMSpartial least squares-discriminant analysis |
| spellingShingle | Xuehong Qiu Li Cao Richou Han Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products Molecules <i>Ophiocordyceps sinensis</i> <i>Thitarodes</i> hosts Chinese cordyceps volatile compounds HS-SPME GC×GC-QTOFMS partial least squares-discriminant analysis |
| title | Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products |
| title_full | Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products |
| title_fullStr | Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products |
| title_full_unstemmed | Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products |
| title_short | Analysis of Volatile Components in Different <i>Ophiocordyceps sinensis</i> and Insect Host Products |
| title_sort | analysis of volatile components in different i ophiocordyceps sinensis i and insect host products |
| topic | <i>Ophiocordyceps sinensis</i> <i>Thitarodes</i> hosts Chinese cordyceps volatile compounds HS-SPME GC×GC-QTOFMS partial least squares-discriminant analysis |
| url | https://www.mdpi.com/1420-3049/25/7/1603 |
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