Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor
Curli, a type of fimbriae widely distributed in uropathogenic Escherichia coli (UPEC), are involved in adhesion to human bladder cell surfaces and biofilm development. The role of UPEC curli was evaluated in a murine model of urinary tract infection. The aim of this study was to establish the role o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02063/full |
| _version_ | 1818518918555238400 |
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| author | Víctor M. Luna-Pineda Leticia Moreno-Fierros Vicenta Cázares-Domínguez Damaris Ilhuicatzi-Alvarado Sara A. Ochoa Ariadnna Cruz-Córdova Pedro Valencia-Mayoral Alejandra Rodríguez-Leviz Juan Xicohtencatl-Cortes |
| author_facet | Víctor M. Luna-Pineda Leticia Moreno-Fierros Vicenta Cázares-Domínguez Damaris Ilhuicatzi-Alvarado Sara A. Ochoa Ariadnna Cruz-Córdova Pedro Valencia-Mayoral Alejandra Rodríguez-Leviz Juan Xicohtencatl-Cortes |
| author_sort | Víctor M. Luna-Pineda |
| collection | DOAJ |
| description | Curli, a type of fimbriae widely distributed in uropathogenic Escherichia coli (UPEC), are involved in adhesion to human bladder cell surfaces and biofilm development. The role of UPEC curli was evaluated in a murine model of urinary tract infection. The aim of this study was to establish the role of curli in C57BL/6 mice transurethrally infected with curli-producing and non-curli-producing UPEC strains. We confirmed that curli enhanced UPEC colonization in the urinary tract, resulting in damage to both the bladder and kidney. Intranasal immunization with recombinant CsgA protein protected against colonization by curli-producing UPEC in the urinary tract. Quantification of cytokines from urinary tract organs showed increases in interleukin-6 and tumor necrosis factor (TNF) release in the kidneys 48 h postinfection with curli-producing UPEC. By contrast, mice infected with non-curli-producing UPEC showed the highest release of interleukin-6, -10, and -17A and TNF. Curli may obscure other fimbriae and LPS, preventing interactions with Toll-like receptors. When intranasal immunization with recombinant FimH and PapG proteins and subsequent infection with this strain were performed, cytokine quantification showed a decrease in the stimulation and release by the uroepithelium. Thus, curli are amyloid-like fimbriae that enhances colonization in the urinary tract and a possible fitness factor. |
| first_indexed | 2024-12-11T01:16:52Z |
| format | Article |
| id | doaj.art-90a451bc67564e87afd14dd771845990 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T01:16:52Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-90a451bc67564e87afd14dd7718459902022-12-22T01:25:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02063484204Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness FactorVíctor M. Luna-Pineda0Leticia Moreno-Fierros1Vicenta Cázares-Domínguez2Damaris Ilhuicatzi-Alvarado3Sara A. Ochoa4Ariadnna Cruz-Córdova5Pedro Valencia-Mayoral6Alejandra Rodríguez-Leviz7Juan Xicohtencatl-Cortes8Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoLaboratorio de Inmunidad en Mucosas, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoLaboratorio de Inmunidad en Mucosas, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoDepartamento de Patología, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoDepartamento de Patología, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoCurli, a type of fimbriae widely distributed in uropathogenic Escherichia coli (UPEC), are involved in adhesion to human bladder cell surfaces and biofilm development. The role of UPEC curli was evaluated in a murine model of urinary tract infection. The aim of this study was to establish the role of curli in C57BL/6 mice transurethrally infected with curli-producing and non-curli-producing UPEC strains. We confirmed that curli enhanced UPEC colonization in the urinary tract, resulting in damage to both the bladder and kidney. Intranasal immunization with recombinant CsgA protein protected against colonization by curli-producing UPEC in the urinary tract. Quantification of cytokines from urinary tract organs showed increases in interleukin-6 and tumor necrosis factor (TNF) release in the kidneys 48 h postinfection with curli-producing UPEC. By contrast, mice infected with non-curli-producing UPEC showed the highest release of interleukin-6, -10, and -17A and TNF. Curli may obscure other fimbriae and LPS, preventing interactions with Toll-like receptors. When intranasal immunization with recombinant FimH and PapG proteins and subsequent infection with this strain were performed, cytokine quantification showed a decrease in the stimulation and release by the uroepithelium. Thus, curli are amyloid-like fimbriae that enhances colonization in the urinary tract and a possible fitness factor.https://www.frontiersin.org/article/10.3389/fmicb.2019.02063/fulluropathogenic Escherichia colicurliurinary tract infectionfitness factorCsgA protein |
| spellingShingle | Víctor M. Luna-Pineda Leticia Moreno-Fierros Vicenta Cázares-Domínguez Damaris Ilhuicatzi-Alvarado Sara A. Ochoa Ariadnna Cruz-Córdova Pedro Valencia-Mayoral Alejandra Rodríguez-Leviz Juan Xicohtencatl-Cortes Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor Frontiers in Microbiology uropathogenic Escherichia coli curli urinary tract infection fitness factor CsgA protein |
| title | Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor |
| title_full | Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor |
| title_fullStr | Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor |
| title_full_unstemmed | Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor |
| title_short | Curli of Uropathogenic Escherichia coli Enhance Urinary Tract Colonization as a Fitness Factor |
| title_sort | curli of uropathogenic escherichia coli enhance urinary tract colonization as a fitness factor |
| topic | uropathogenic Escherichia coli curli urinary tract infection fitness factor CsgA protein |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02063/full |
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