The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants
Abstract Background The SnRK2s (Plant specific protein kinase) are involved in various biological processes, such as plant defense and environmental challenges. In Arabidopsis, AtSnRK2s regulate the expression of some metal transporters. For example, AtSnRK2.4 plays a role in the regulation of Arabi...
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5589-1 |
| _version_ | 1811335756607651840 |
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| author | Ruijiao Wang Chao Wang Qin Yao Xue Xiao Xing Fan Lina Sha Jian Zeng Houyang Kang Haiqin Zhang Yonghong Zhou Yi Wang |
| author_facet | Ruijiao Wang Chao Wang Qin Yao Xue Xiao Xing Fan Lina Sha Jian Zeng Houyang Kang Haiqin Zhang Yonghong Zhou Yi Wang |
| author_sort | Ruijiao Wang |
| collection | DOAJ |
| description | Abstract Background The SnRK2s (Plant specific protein kinase) are involved in various biological processes, such as plant defense and environmental challenges. In Arabidopsis, AtSnRK2s regulate the expression of some metal transporters. For example, AtSnRK2.4 plays a role in the regulation of Arabidopsis tolerance to Cd; AtSnRK2.2 and AtSnRK2.3 are involved in Cd uptake and translocation. However, the functions of their homologs, TpSnRK2.10 and TpSnRK2.11 from dwarf Polish wheat are unknown. Results TpSnRK2.11 encodes a cytoplasmic protein. TpSnRK2.10 and TpSnRK2.11 have different expression patterns at different growth stages. Expression of TpSnRK2.10 increased yeast’s sensitivity to Cd; conversely, expression of TpSnRK2.11 enhanced yeast’s tolerance to Cd. Overexpression of TpSnRK2.10 or TpSnRK2.11 did not affect Cd sensitivity in Arabidopsis, but significantly increased Cd accumulation in roots and shoots, and Cd translocation from roots to shoots. While, Fe accumulation was significantly increased in roots but decreased in shoots by overexpression of TpSnRK2.10; opposite results were observed in TpSnRK2.11-overexpressing lines. Subcellular distribution analysis found that overexpression of TpSnRK2.10 and TpSnRK2.11 increased Cd concentration in cell wall and organelle fractions of roots and shoots; meanwhile, they also differentially influenced Fe distribution. Conclusions These results indicated that TpSnRK2.10 and TpSnRK2.11 are involved in the uptakes and the translocations of Cd and Fe, possibly by regulating the expression of AtNRAMP1 and AtHMA4, and other genes involved in the synthesis of phytochelatins or hemicellolosic polysaccharides. |
| first_indexed | 2024-04-13T17:29:42Z |
| format | Article |
| id | doaj.art-90a9936881fe46228652c8539c7d61fb |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T17:29:42Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-90a9936881fe46228652c8539c7d61fb2022-12-22T02:37:37ZengBMCBMC Genomics1471-21642019-03-0120111310.1186/s12864-019-5589-1The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plantsRuijiao Wang0Chao Wang1Qin Yao2Xue Xiao3Xing Fan4Lina Sha5Jian Zeng6Houyang Kang7Haiqin Zhang8Yonghong Zhou9Yi Wang10Triticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityCollege of Resources, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityAbstract Background The SnRK2s (Plant specific protein kinase) are involved in various biological processes, such as plant defense and environmental challenges. In Arabidopsis, AtSnRK2s regulate the expression of some metal transporters. For example, AtSnRK2.4 plays a role in the regulation of Arabidopsis tolerance to Cd; AtSnRK2.2 and AtSnRK2.3 are involved in Cd uptake and translocation. However, the functions of their homologs, TpSnRK2.10 and TpSnRK2.11 from dwarf Polish wheat are unknown. Results TpSnRK2.11 encodes a cytoplasmic protein. TpSnRK2.10 and TpSnRK2.11 have different expression patterns at different growth stages. Expression of TpSnRK2.10 increased yeast’s sensitivity to Cd; conversely, expression of TpSnRK2.11 enhanced yeast’s tolerance to Cd. Overexpression of TpSnRK2.10 or TpSnRK2.11 did not affect Cd sensitivity in Arabidopsis, but significantly increased Cd accumulation in roots and shoots, and Cd translocation from roots to shoots. While, Fe accumulation was significantly increased in roots but decreased in shoots by overexpression of TpSnRK2.10; opposite results were observed in TpSnRK2.11-overexpressing lines. Subcellular distribution analysis found that overexpression of TpSnRK2.10 and TpSnRK2.11 increased Cd concentration in cell wall and organelle fractions of roots and shoots; meanwhile, they also differentially influenced Fe distribution. Conclusions These results indicated that TpSnRK2.10 and TpSnRK2.11 are involved in the uptakes and the translocations of Cd and Fe, possibly by regulating the expression of AtNRAMP1 and AtHMA4, and other genes involved in the synthesis of phytochelatins or hemicellolosic polysaccharides.http://link.springer.com/article/10.1186/s12864-019-5589-1WheatTpSnRK2CdBiological functionExpression pattern |
| spellingShingle | Ruijiao Wang Chao Wang Qin Yao Xue Xiao Xing Fan Lina Sha Jian Zeng Houyang Kang Haiqin Zhang Yonghong Zhou Yi Wang The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants BMC Genomics Wheat TpSnRK2 Cd Biological function Expression pattern |
| title | The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants |
| title_full | The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants |
| title_fullStr | The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants |
| title_full_unstemmed | The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants |
| title_short | The polish wheat (Triticum polonicum L.) TpSnRK2.10 and TpSnRK2.11 meditate the accumulation and the distribution of cd and Fe in transgenic Arabidopsis plants |
| title_sort | polish wheat triticum polonicum l tpsnrk2 10 and tpsnrk2 11 meditate the accumulation and the distribution of cd and fe in transgenic arabidopsis plants |
| topic | Wheat TpSnRK2 Cd Biological function Expression pattern |
| url | http://link.springer.com/article/10.1186/s12864-019-5589-1 |
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