Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i>
<i>Sanghuangporus</i> <i>baumii</i> is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and its growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic a...
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/648 |
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| author | Zengcai Liu Xinyu Tong Ruipeng Liu Li Zou |
| author_facet | Zengcai Liu Xinyu Tong Ruipeng Liu Li Zou |
| author_sort | Zengcai Liu |
| collection | DOAJ |
| description | <i>Sanghuangporus</i> <i>baumii</i> is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and its growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic analyses, we found four terpenoid hormones and a central gene, isopentenyl diphosphate isomerase (<i>IDI</i>), involved in growth and development. Additionally, an exogenous hormone test was used to further confirm the importance of the four terpenoid hormones. Finally, hormone content determination and qRT−PCR were performed to explore the growth and development mechanism; we found thatcis-zeatin (CZ) plays a major role in the mycelia stage, trans-zeatin (TZ) and gibberellin A4 (GA4) are important in the primordia stage, GA4 is crucial for the fruiting bodies stage, and abscisic acid (ABA) may be a marker of maturity. The <i>IDI</i> gene was also found to affectterpenoid hormone content by regulating the relative gene transcript levels, thereby controlling morphological changes in <i>S. baumii</i>. Our results revealthe growth and development mechanisms of <i>S. baumii</i> and may promote the breeding and utilisation of high-quality varieties. |
| first_indexed | 2024-03-09T10:16:31Z |
| format | Article |
| id | doaj.art-90aa3beeb3ad41c6ac60328a8a6ac22c |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:16:31Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-90aa3beeb3ad41c6ac60328a8a6ac22c2023-12-01T22:19:45ZengMDPI AGJournal of Fungi2309-608X2022-06-018764810.3390/jof8070648Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i>Zengcai Liu0Xinyu Tong1Ruipeng Liu2Li Zou3College of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, ChinaCollege of Forestry, Northeast Forestry University, Harbin 150040, China<i>Sanghuangporus</i> <i>baumii</i> is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and its growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic analyses, we found four terpenoid hormones and a central gene, isopentenyl diphosphate isomerase (<i>IDI</i>), involved in growth and development. Additionally, an exogenous hormone test was used to further confirm the importance of the four terpenoid hormones. Finally, hormone content determination and qRT−PCR were performed to explore the growth and development mechanism; we found thatcis-zeatin (CZ) plays a major role in the mycelia stage, trans-zeatin (TZ) and gibberellin A4 (GA4) are important in the primordia stage, GA4 is crucial for the fruiting bodies stage, and abscisic acid (ABA) may be a marker of maturity. The <i>IDI</i> gene was also found to affectterpenoid hormone content by regulating the relative gene transcript levels, thereby controlling morphological changes in <i>S. baumii</i>. Our results revealthe growth and development mechanisms of <i>S. baumii</i> and may promote the breeding and utilisation of high-quality varieties.https://www.mdpi.com/2309-608X/8/7/648<i>Sanghuangporus</i> <i>baumii</i>growth and developmentmetabolome and transcriptomeisopentenyl diphosphate isomeraseterpenoid biosynthesishormone |
| spellingShingle | Zengcai Liu Xinyu Tong Ruipeng Liu Li Zou Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> Journal of Fungi <i>Sanghuangporus</i> <i>baumii</i> growth and development metabolome and transcriptome isopentenyl diphosphate isomerase terpenoid biosynthesis hormone |
| title | Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> |
| title_full | Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> |
| title_fullStr | Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> |
| title_full_unstemmed | Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> |
| title_short | Metabolome and Transcriptome Profiling Reveal That Four Terpenoid Hormones Dominate the Growth and Development of <i>Sanghuangporus</i> <i>baumii</i> |
| title_sort | metabolome and transcriptome profiling reveal that four terpenoid hormones dominate the growth and development of i sanghuangporus i i baumii i |
| topic | <i>Sanghuangporus</i> <i>baumii</i> growth and development metabolome and transcriptome isopentenyl diphosphate isomerase terpenoid biosynthesis hormone |
| url | https://www.mdpi.com/2309-608X/8/7/648 |
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