Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells
In this work is presented the synthesis of the inclusion complexes of amygdalin with β-cyclodextrin by three methods: kneading, co-precipitation and freeze drying and their characterization.The inclusion compounds were synthesized using a molar ratio of amygdalin: β-cyclodextrin of 1:1. Synthesized...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535220302410 |
| _version_ | 1818306371788996608 |
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| author | Eleonora Marian Narcis Duteanu Laura Vicas Gerlinde Rusu Tunde Jurca Mariana Muresan Otilia Micle Adriana Corina Hangan Roxana Liana Stan Corina Ionescu Bogdan Sevastre Emöke Páll |
| author_facet | Eleonora Marian Narcis Duteanu Laura Vicas Gerlinde Rusu Tunde Jurca Mariana Muresan Otilia Micle Adriana Corina Hangan Roxana Liana Stan Corina Ionescu Bogdan Sevastre Emöke Páll |
| author_sort | Eleonora Marian |
| collection | DOAJ |
| description | In this work is presented the synthesis of the inclusion complexes of amygdalin with β-cyclodextrin by three methods: kneading, co-precipitation and freeze drying and their characterization.The inclusion compounds were synthesized using a molar ratio of amygdalin: β-cyclodextrin of 1:1. Synthesized compounds were analyzed by FTIR spectroscopy, X-rays, thermal analysis and the results confirm the formation of inclusion compounds by amygdalin with β-cyclodextrin.The studies carried out have shown the protective effect of amygdalin-β-cyclodextrin compounds obtained by freeze drying, kneading and coprecipitation against free radicals (SOD-like activity in vivo) and the results of in vitro cell cytotoxicity of the compounds on HeLa cell line compared to pure amygdalin. Compound obtained by freeze drying has the best SOD-like activity and cytotoxicity on HeLa tumor cells, so it may be considered as potential therapeutic agent. |
| first_indexed | 2024-12-13T06:41:26Z |
| format | Article |
| id | doaj.art-90aa8df98e1d4086ac308338cf07c09f |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-13T06:41:26Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-90aa8df98e1d4086ac308338cf07c09f2022-12-21T23:56:25ZengElsevierArabian Journal of Chemistry1878-53522020-08-0113868286837Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cellsEleonora Marian0Narcis Duteanu1Laura Vicas2Gerlinde Rusu3Tunde Jurca4Mariana Muresan5Otilia Micle6Adriana Corina Hangan7Roxana Liana Stan8Corina Ionescu9Bogdan Sevastre10Emöke Páll11University of Oradea, Medicine and Pharmacy Faculty, 1 December Square, 410028 Oradea, RomaniaUniversity Politehnica of Timisoara, Faculty of Industrial Chemistry and Environmental Engineering, 2 Victoria Square, 300006 Timisoara, Romania; Corresponding author.University of Oradea, Medicine and Pharmacy Faculty, 1 December Square, 410028 Oradea, RomaniaUniversity Politehnica of Timisoara, Faculty of Industrial Chemistry and Environmental Engineering, 2 Victoria Square, 300006 Timisoara, RomaniaUniversity of Oradea, Medicine and Pharmacy Faculty, 1 December Square, 410028 Oradea, RomaniaUniversity of Oradea, Medicine and Pharmacy Faculty, 1 December Square, 410028 Oradea, RomaniaUniversity of Oradea, Medicine and Pharmacy Faculty, 1 December Square, 410028 Oradea, RomaniaDepartment of Inorganic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Iuliu-Hațieganu” Cluj-Napoca, Cluj-Napoca 4000012, RomaniaDepartment of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, University of Medicine and Pharmacy ,Iuliu-Hațieganu” Cluj-Napoca, Cluj-Napoca 400012, RomaniaDepartment of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, University of Medicine and Pharmacy ,Iuliu-Hațieganu” Cluj-Napoca, Cluj-Napoca 400012, RomaniaParaclinic Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca 400372, RomaniaParaclinic Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca 400372, RomaniaIn this work is presented the synthesis of the inclusion complexes of amygdalin with β-cyclodextrin by three methods: kneading, co-precipitation and freeze drying and their characterization.The inclusion compounds were synthesized using a molar ratio of amygdalin: β-cyclodextrin of 1:1. Synthesized compounds were analyzed by FTIR spectroscopy, X-rays, thermal analysis and the results confirm the formation of inclusion compounds by amygdalin with β-cyclodextrin.The studies carried out have shown the protective effect of amygdalin-β-cyclodextrin compounds obtained by freeze drying, kneading and coprecipitation against free radicals (SOD-like activity in vivo) and the results of in vitro cell cytotoxicity of the compounds on HeLa cell line compared to pure amygdalin. Compound obtained by freeze drying has the best SOD-like activity and cytotoxicity on HeLa tumor cells, so it may be considered as potential therapeutic agent.http://www.sciencedirect.com/science/article/pii/S1878535220302410Amygdalinβ-cyclodextrinInclusion compoundsFree radicalsCytotoxicity |
| spellingShingle | Eleonora Marian Narcis Duteanu Laura Vicas Gerlinde Rusu Tunde Jurca Mariana Muresan Otilia Micle Adriana Corina Hangan Roxana Liana Stan Corina Ionescu Bogdan Sevastre Emöke Páll Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells Arabian Journal of Chemistry Amygdalin β-cyclodextrin Inclusion compounds Free radicals Cytotoxicity |
| title | Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells |
| title_full | Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells |
| title_fullStr | Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells |
| title_full_unstemmed | Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells |
| title_short | Synthesis, characterization of inclusion compounds of amygdalin with β-cyclodextrin and sod-like activity and cytotoxicity on hela tumor cells |
| title_sort | synthesis characterization of inclusion compounds of amygdalin with β cyclodextrin and sod like activity and cytotoxicity on hela tumor cells |
| topic | Amygdalin β-cyclodextrin Inclusion compounds Free radicals Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S1878535220302410 |
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