Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances
The aim of this study was to examine the effect of orthodontic appliances on antimicrobial and antioxidant capacity of the saliva. For this purpose seventeen children (11 girls, 6 boys) aged between 12–20 years with no systemic disease were selected. Unstimulated whole saliva sample was collected be...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2002-08-01
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| Series: | Turkish Journal of Orthodontics |
| Subjects: | |
| Online Access: |
http://www.turkjorthod.org/archives/archive-detail/article-preview/salva-lysozyme-levels-and-antox-dant-enzyme-actvty/53965
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| _version_ | 1797767273108209664 |
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| author | Hülya Kılıçoğlu Koray Gümüştaş Figen Gürdöl |
| author_facet | Hülya Kılıçoğlu Koray Gümüştaş Figen Gürdöl |
| author_sort | Hülya Kılıçoğlu |
| collection | DOAJ |
| description | The aim of this study was to examine the effect of orthodontic appliances on antimicrobial and antioxidant capacity of the saliva. For this purpose seventeen children (11 girls, 6 boys) aged between 12–20 years with no systemic disease were selected. Unstimulated whole saliva sample was collected before orthodontic treatment of each patient. The sample collection was repeated on third and sixth month after the placement of brackets, bands and wires. AH saliva samples were kept at −35°C until studied. The antimicrobial protein lysozyme assays were performed by the method of Osserman and Lawlor. The antioxidant defense enzyme superoxide dismutase (SOD) activity was measured using the nitroblue tetrazolium assay. The protein content was measured by the method of Lowry. The data were evaluated statistically with paired-t test. Three months after the placement of orthodontic appliances, lysozyme levels were unchanged; however they were significantly reduced on the sixth month of treatment (1.11 ±0.62 mg/mg protein vs 0.63±0.41 mg/mg protein; p<0.05). On the other hand, SOD activity was continuously elevated following the treatment. Mean SOD activity in saliva was 3.93±1.73 U/mg protein on the third month, and 5.21+2.19 U/mg protein on the sixth month which were both significantly higher than the basal level (2.83+1.26 U/mg protein; p<0.01). No significant difference in the total protein content in unstimulated saliva was observed between the three stages of the study. It is concluded that fixed orthodontic appliances may alter the antibacterial and antioxidant capacity of the saliva following a six-month treatment. |
| first_indexed | 2024-03-12T20:37:20Z |
| format | Article |
| id | doaj.art-90ab209b7c574381a8ca672f0492d9e8 |
| institution | Directory Open Access Journal |
| issn | 2528-9659 2148-9505 |
| language | English |
| last_indexed | 2024-03-12T20:37:20Z |
| publishDate | 2002-08-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Turkish Journal of Orthodontics |
| spelling | doaj.art-90ab209b7c574381a8ca672f0492d9e82023-08-01T12:54:53ZengGalenos YayineviTurkish Journal of Orthodontics2528-96592148-95052002-08-01152879110.13076/1300-3550-15-2-8713049054Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc ApplıancesHülya Kılıçoğlu0Koray Gümüştaş1Figen Gürdöl2 Department of Orthodontics, Faculty of Dentistry, Istanbul University, Çapa Department of Biochemistry, Cerrahpaşa Faculty of Medicine, Istanbul University, Cerrahpaşa Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Çapa The aim of this study was to examine the effect of orthodontic appliances on antimicrobial and antioxidant capacity of the saliva. For this purpose seventeen children (11 girls, 6 boys) aged between 12–20 years with no systemic disease were selected. Unstimulated whole saliva sample was collected before orthodontic treatment of each patient. The sample collection was repeated on third and sixth month after the placement of brackets, bands and wires. AH saliva samples were kept at −35°C until studied. The antimicrobial protein lysozyme assays were performed by the method of Osserman and Lawlor. The antioxidant defense enzyme superoxide dismutase (SOD) activity was measured using the nitroblue tetrazolium assay. The protein content was measured by the method of Lowry. The data were evaluated statistically with paired-t test. Three months after the placement of orthodontic appliances, lysozyme levels were unchanged; however they were significantly reduced on the sixth month of treatment (1.11 ±0.62 mg/mg protein vs 0.63±0.41 mg/mg protein; p<0.05). On the other hand, SOD activity was continuously elevated following the treatment. Mean SOD activity in saliva was 3.93±1.73 U/mg protein on the third month, and 5.21+2.19 U/mg protein on the sixth month which were both significantly higher than the basal level (2.83+1.26 U/mg protein; p<0.01). No significant difference in the total protein content in unstimulated saliva was observed between the three stages of the study. It is concluded that fixed orthodontic appliances may alter the antibacterial and antioxidant capacity of the saliva following a six-month treatment. http://www.turkjorthod.org/archives/archive-detail/article-preview/salva-lysozyme-levels-and-antox-dant-enzyme-actvty/53965 salivalysozymesuperoxide dismutasefixed orthodontic appliances |
| spellingShingle | Hülya Kılıçoğlu Koray Gümüştaş Figen Gürdöl Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances Turkish Journal of Orthodontics saliva lysozyme superoxide dismutase fixed orthodontic appliances |
| title | Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances |
| title_full | Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances |
| title_fullStr | Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances |
| title_full_unstemmed | Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances |
| title_short | Salıva Lysozyme Levels And Antıoxı-Dant Enzyme Actıvıty In Patıents Wıth Fıxed Orthodontıc Applıances |
| title_sort | saliva lysozyme levels and antioxi dant enzyme activity in patients with fixed orthodontic appliances |
| topic | saliva lysozyme superoxide dismutase fixed orthodontic appliances |
| url |
http://www.turkjorthod.org/archives/archive-detail/article-preview/salva-lysozyme-levels-and-antox-dant-enzyme-actvty/53965
|
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