Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i>
Aquatic habitats are very frequently polluted with different kinds of xenobiotics, including heavy metals. For biomonitoring studies of aquatic pollution, algae are frequently used, as they do not contain protective cuticle on the surface of their thalli and can accumulate pollutants over the whole...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/20/3607 |
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| author | Martin Bačkor Michal Goga Pragya Singh Viktória Tuptová |
| author_facet | Martin Bačkor Michal Goga Pragya Singh Viktória Tuptová |
| author_sort | Martin Bačkor |
| collection | DOAJ |
| description | Aquatic habitats are very frequently polluted with different kinds of xenobiotics, including heavy metals. For biomonitoring studies of aquatic pollution, algae are frequently used, as they do not contain protective cuticle on the surface of their thalli and can accumulate pollutants over the whole surface of thalli. However, this is a feature of most cryptogams. For this reason, we assessed the sensitivity of the aquatic moss <i>Taxiphyllum barbieri</i> (Java moss) to copper excess in a short-term study. Moss <i>T. barbieri</i> belongs to the common aquatic plants originating from Southeast Asia. To test the sensitivity (or tolerance) of the moss to excess Cu, selected concentrations (50, 250 and 500 µM) were employed in our 24 h studies. Total and intracellular Cu accumulation positively correlated with Cu availability in the water. This total and intracellular Cu accumulation was negatively correlated with decreased intracellular K content. Excess Cu negatively affected the composition of assimilation pigments and soluble proteins. Cu caused increased peroxidation of membrane lipids assessed using TBARS assay. Excess Cu decreased GSH to GSSG ratio and ascorbic acid content. We did not observe phytochelatin synthesis in this moss. The roles of selected amino acids, their intermediates and derivatives, as well as S-containing nucleosides and phenolic acids in Cu homeostasis and toxicity or tolerance were evaluated. We assume that this moss has potential for future employment in water quality evaluation. |
| first_indexed | 2024-03-10T20:56:46Z |
| format | Article |
| id | doaj.art-90ac66a520be43fb9d185019ee40e114 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:56:46Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-90ac66a520be43fb9d185019ee40e1142023-11-19T17:49:25ZengMDPI AGPlants2223-77472023-10-011220360710.3390/plants12203607Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i>Martin Bačkor0Michal Goga1Pragya Singh2Viktória Tuptová3Department of Biochemistry and Biotechnology, Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, Šafárik University, Mánesova 23, 041 67 Košice, SlovakiaDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, Šafárik University, Mánesova 23, 041 67 Košice, SlovakiaDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, Šafárik University, Mánesova 23, 041 67 Košice, SlovakiaAquatic habitats are very frequently polluted with different kinds of xenobiotics, including heavy metals. For biomonitoring studies of aquatic pollution, algae are frequently used, as they do not contain protective cuticle on the surface of their thalli and can accumulate pollutants over the whole surface of thalli. However, this is a feature of most cryptogams. For this reason, we assessed the sensitivity of the aquatic moss <i>Taxiphyllum barbieri</i> (Java moss) to copper excess in a short-term study. Moss <i>T. barbieri</i> belongs to the common aquatic plants originating from Southeast Asia. To test the sensitivity (or tolerance) of the moss to excess Cu, selected concentrations (50, 250 and 500 µM) were employed in our 24 h studies. Total and intracellular Cu accumulation positively correlated with Cu availability in the water. This total and intracellular Cu accumulation was negatively correlated with decreased intracellular K content. Excess Cu negatively affected the composition of assimilation pigments and soluble proteins. Cu caused increased peroxidation of membrane lipids assessed using TBARS assay. Excess Cu decreased GSH to GSSG ratio and ascorbic acid content. We did not observe phytochelatin synthesis in this moss. The roles of selected amino acids, their intermediates and derivatives, as well as S-containing nucleosides and phenolic acids in Cu homeostasis and toxicity or tolerance were evaluated. We assume that this moss has potential for future employment in water quality evaluation.https://www.mdpi.com/2223-7747/12/20/3607amino acidsantioxidantscopperglutathionemetalsmosses |
| spellingShingle | Martin Bačkor Michal Goga Pragya Singh Viktória Tuptová Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> Plants amino acids antioxidants copper glutathione metals mosses |
| title | Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> |
| title_full | Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> |
| title_fullStr | Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> |
| title_full_unstemmed | Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> |
| title_short | Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss <i>Taxiphyllum barbieri</i> |
| title_sort | mechanisms of copper toxicity and tolerance in the aquatic moss i taxiphyllum barbieri i |
| topic | amino acids antioxidants copper glutathione metals mosses |
| url | https://www.mdpi.com/2223-7747/12/20/3607 |
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