The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa
Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this st...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00750/full |
| _version_ | 1818012937467461632 |
|---|---|
| author | Xianyun Sun Xianyun Sun Fei Wang Fei Wang Nan Lan Nan Lan Bo Liu Chengcheng Hu Chengcheng Hu Wei Xue Wei Xue Zhenying Zhang Shaojie Li Shaojie Li |
| author_facet | Xianyun Sun Xianyun Sun Fei Wang Fei Wang Nan Lan Nan Lan Bo Liu Chengcheng Hu Chengcheng Hu Wei Xue Wei Xue Zhenying Zhang Shaojie Li Shaojie Li |
| author_sort | Xianyun Sun |
| collection | DOAJ |
| description | Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several conidiation related genes are positively regulated by ADA-6, including genes that positively regulate conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in conidiation, sexual development, and oxidative stress response of N. crassa. |
| first_indexed | 2024-04-14T06:27:12Z |
| format | Article |
| id | doaj.art-90aeb2fd67f5400381ac094eb0b74bd8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T06:27:12Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-90aeb2fd67f5400381ac094eb0b74bd82022-12-22T02:07:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-04-011010.3389/fmicb.2019.00750441260The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassaXianyun Sun0Xianyun Sun1Fei Wang2Fei Wang3Nan Lan4Nan Lan5Bo Liu6Chengcheng Hu7Chengcheng Hu8Wei Xue9Wei Xue10Zhenying Zhang11Shaojie Li12Shaojie Li13State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Food Science and Engineering, Qilu University of Technology, Jinan, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCollege of Food Science and Engineering, Qilu University of Technology, Jinan, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaConidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several conidiation related genes are positively regulated by ADA-6, including genes that positively regulate conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in conidiation, sexual development, and oxidative stress response of N. crassa.https://www.frontiersin.org/article/10.3389/fmicb.2019.00750/fullconidiationada-6sexual developmentoxidative stress responseNeurospora crassa |
| spellingShingle | Xianyun Sun Xianyun Sun Fei Wang Fei Wang Nan Lan Nan Lan Bo Liu Chengcheng Hu Chengcheng Hu Wei Xue Wei Xue Zhenying Zhang Shaojie Li Shaojie Li The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa Frontiers in Microbiology conidiation ada-6 sexual development oxidative stress response Neurospora crassa |
| title | The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa |
| title_full | The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa |
| title_fullStr | The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa |
| title_full_unstemmed | The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa |
| title_short | The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa |
| title_sort | zn ii 2cys6 type transcription factor ada 6 regulates conidiation sexual development and oxidative stress response in neurospora crassa |
| topic | conidiation ada-6 sexual development oxidative stress response Neurospora crassa |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00750/full |
| work_keys_str_mv | AT xianyunsun theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT xianyunsun theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT feiwang theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT feiwang theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT nanlan theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT nanlan theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT boliu theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT chengchenghu theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT chengchenghu theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT weixue theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT weixue theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT zhenyingzhang theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT shaojieli theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT shaojieli theznii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT xianyunsun znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT xianyunsun znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT feiwang znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT feiwang znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT nanlan znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT nanlan znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT boliu znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT chengchenghu znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT chengchenghu znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT weixue znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT weixue znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT zhenyingzhang znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT shaojieli znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa AT shaojieli znii2cys6typetranscriptionfactorada6regulatesconidiationsexualdevelopmentandoxidativestressresponseinneurosporacrassa |