Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture
Abstract In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation metho...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-04-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202103554 |
| _version_ | 1818348973097746432 |
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| author | Dirk Rommel Matthias Mork Sitara Vedaraman Céline Bastard Luis P. B. Guerzoni Yonca Kittel Rostislav Vinokur Nikolai Born Tamás Haraszti Laura De Laporte |
| author_facet | Dirk Rommel Matthias Mork Sitara Vedaraman Céline Bastard Luis P. B. Guerzoni Yonca Kittel Rostislav Vinokur Nikolai Born Tamás Haraszti Laura De Laporte |
| author_sort | Dirk Rommel |
| collection | DOAJ |
| description | Abstract In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation method by photoinitiated free‐radical polymerization of star‐polyethylene glycol‐acrylate with glycidyl methacrylate or 2‐aminoethyl methacrylate comonomers. The resulting complementary epoxy‐ and amine‐functionalized microgels assemble and interlink with each other via a ring opening reaction, resulting in macroporous constructs with pores up to several hundreds of micrometers. The level of crosslinking depends on the functionalization degree of the microgels, which also affects the stiffness and cell adhesiveness of the microgels when modified with the cell‐adhesive GRGDS‐PC peptide. Therefore, 3D spreading and growth of cells inside the macroporous structure is influenced not only by the presence of macropores but also by the mechanical and biochemical properties of the individual microgels. |
| first_indexed | 2024-12-13T17:58:33Z |
| format | Article |
| id | doaj.art-90b3d059ab61417cad2fbaa526676213 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-13T17:58:33Z |
| publishDate | 2022-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-90b3d059ab61417cad2fbaa5266762132022-12-21T23:36:18ZengWileyAdvanced Science2198-38442022-04-01910n/an/a10.1002/advs.202103554Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell CultureDirk Rommel0Matthias Mork1Sitara Vedaraman2Céline Bastard3Luis P. B. Guerzoni4Yonca Kittel5Rostislav Vinokur6Nikolai Born7Tamás Haraszti8Laura De Laporte9DWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyOptics11Life Amsterdam 1081 NetherlandsDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyDWI – Leibniz Institute for Interactive Materials Aachen 52074 GermanyAbstract In this work, a two component microgel assembly using soft anisometric microgels that interlink to create a 3D macroporous construct for cell growth is reported. Reactive microgel rods with variable aspect ratio are produced via microfluidics in a continuous plug‐flow on‐chip gelation method by photoinitiated free‐radical polymerization of star‐polyethylene glycol‐acrylate with glycidyl methacrylate or 2‐aminoethyl methacrylate comonomers. The resulting complementary epoxy‐ and amine‐functionalized microgels assemble and interlink with each other via a ring opening reaction, resulting in macroporous constructs with pores up to several hundreds of micrometers. The level of crosslinking depends on the functionalization degree of the microgels, which also affects the stiffness and cell adhesiveness of the microgels when modified with the cell‐adhesive GRGDS‐PC peptide. Therefore, 3D spreading and growth of cells inside the macroporous structure is influenced not only by the presence of macropores but also by the mechanical and biochemical properties of the individual microgels.https://doi.org/10.1002/advs.2021035543D cell culturemacroporous microgel scaffoldsmicrofluidicsrod‐shaped microgelstissue engineering |
| spellingShingle | Dirk Rommel Matthias Mork Sitara Vedaraman Céline Bastard Luis P. B. Guerzoni Yonca Kittel Rostislav Vinokur Nikolai Born Tamás Haraszti Laura De Laporte Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture Advanced Science 3D cell culture macroporous microgel scaffolds microfluidics rod‐shaped microgels tissue engineering |
| title | Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture |
| title_full | Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture |
| title_fullStr | Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture |
| title_full_unstemmed | Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture |
| title_short | Functionalized Microgel Rods Interlinked into Soft Macroporous Structures for 3D Cell Culture |
| title_sort | functionalized microgel rods interlinked into soft macroporous structures for 3d cell culture |
| topic | 3D cell culture macroporous microgel scaffolds microfluidics rod‐shaped microgels tissue engineering |
| url | https://doi.org/10.1002/advs.202103554 |
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