Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation
In this study, the gamma ray-induced Maillard reaction method was carried out for chitosan (CTS) and glucosamine (GA) to improve the water solubility and antibacterial activity. The mixture solution of CTS and GA was exposed to gamma rays at a dose of 25 kGy and freeze-dried to obtain a Maillard rea...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/22/4397 |
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| author | Anh Quoc Le Van Phu Dang Ngoc Duy Nguyen Chi Thuan Nguyen Quoc Hien Nguyen |
| author_facet | Anh Quoc Le Van Phu Dang Ngoc Duy Nguyen Chi Thuan Nguyen Quoc Hien Nguyen |
| author_sort | Anh Quoc Le |
| collection | DOAJ |
| description | In this study, the gamma ray-induced Maillard reaction method was carried out for chitosan (CTS) and glucosamine (GA) to improve the water solubility and antibacterial activity. The mixture solution of CTS and GA was exposed to gamma rays at a dose of 25 kGy and freeze-dried to obtain a Maillard reaction product (MRP) powder. The physicochemical and biological properties of the CTS-GA MRP powder were investigated. The CTS-GA MRP powder expressed good solubility at a concentration of 0.05 g/mL. In addition, the result of the antibacterial activity test against <i>Escherichia coli</i> revealed that the CTS-GA MRP powder exhibited highly antibacterial activity at pH 7; in particular, bacterial density was reduced by over 4 logs. Furthermore, the cytotoxicity test of the CTS-GA MRP powder on mouse fibroblast cells (L929) showed non-cytotoxicity with high cell viability (>90%) at concentrations of 0.1–1 mg/mL. Owing to the high antibacterial activity and low cytotoxicity, the water-soluble CTS-GA MRP powder can be used as a favorable natural preservative for food and cosmetics. |
| first_indexed | 2024-03-09T16:30:34Z |
| format | Article |
| id | doaj.art-90b6a308da584198a0f17e96daf4f02a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T16:30:34Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-90b6a308da584198a0f17e96daf4f02a2023-11-24T15:02:28ZengMDPI AGPolymers2073-43602023-11-011522439710.3390/polym15224397Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray IrradiationAnh Quoc Le0Van Phu Dang1Ngoc Duy Nguyen2Chi Thuan Nguyen3Quoc Hien Nguyen4Faculty of Biology-Biotechnology, University of Science, Ho Chi Minh City 700000, VietnamResearch and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, Ho Chi Minh City 700000, VietnamResearch and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, Ho Chi Minh City 700000, VietnamResearch and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, Ho Chi Minh City 700000, VietnamVietnam Atomic Energy Institute, Hanoi 100000, VietnamIn this study, the gamma ray-induced Maillard reaction method was carried out for chitosan (CTS) and glucosamine (GA) to improve the water solubility and antibacterial activity. The mixture solution of CTS and GA was exposed to gamma rays at a dose of 25 kGy and freeze-dried to obtain a Maillard reaction product (MRP) powder. The physicochemical and biological properties of the CTS-GA MRP powder were investigated. The CTS-GA MRP powder expressed good solubility at a concentration of 0.05 g/mL. In addition, the result of the antibacterial activity test against <i>Escherichia coli</i> revealed that the CTS-GA MRP powder exhibited highly antibacterial activity at pH 7; in particular, bacterial density was reduced by over 4 logs. Furthermore, the cytotoxicity test of the CTS-GA MRP powder on mouse fibroblast cells (L929) showed non-cytotoxicity with high cell viability (>90%) at concentrations of 0.1–1 mg/mL. Owing to the high antibacterial activity and low cytotoxicity, the water-soluble CTS-GA MRP powder can be used as a favorable natural preservative for food and cosmetics.https://www.mdpi.com/2073-4360/15/22/4397chitosanglucosamineMaillard reactionantibacterial effectcytotoxicity |
| spellingShingle | Anh Quoc Le Van Phu Dang Ngoc Duy Nguyen Chi Thuan Nguyen Quoc Hien Nguyen Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation Polymers chitosan glucosamine Maillard reaction antibacterial effect cytotoxicity |
| title | Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation |
| title_full | Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation |
| title_fullStr | Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation |
| title_full_unstemmed | Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation |
| title_short | Antibacterial Activity against <i>Escherichia coli</i> and Cytotoxicity of Maillard Reaction Product of Chitosan and Glucosamine Prepared by Gamma Co-60 Ray Irradiation |
| title_sort | antibacterial activity against i escherichia coli i and cytotoxicity of maillard reaction product of chitosan and glucosamine prepared by gamma co 60 ray irradiation |
| topic | chitosan glucosamine Maillard reaction antibacterial effect cytotoxicity |
| url | https://www.mdpi.com/2073-4360/15/22/4397 |
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