Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors
BACKGROUND Adoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODS We provide results of a personalized T cell manufacturing program evaluating donor, patient, T cell product, and outcome data. Patient-tailored c...
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| Format: | Article |
| Language: | English |
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American Society for Clinical Investigation
2023-06-01
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| Series: | The Journal of Clinical Investigation |
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| Online Access: | https://doi.org/10.1172/JCI163548 |
| _version_ | 1797634445384089600 |
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| author | Agnes Bonifacius Britta Lamottke Sabine Tischer-Zimmermann Rebecca Schultze-Florey Lilia Goudeva Hans-Gert Heuft Lubomir Arseniev Rita Beier Gernot Beutel Gunnar Cario Birgit Fröhlich Johann Greil Leo Hansmann Justin Hasenkamp Michaela Höfs Patrick Hundsdoerfer Edgar Jost Kinan Kafa Oliver Kriege Nicolaus Kröger Stephan Mathas Roland Meisel Michaela Nathrath Mervi Putkonen Sarina Ravens Hans Christian Reinhardt Elisa Sala Martin G. Sauer Clemens Schmitt Roland Schroers Nina Kristin Steckel Ralf Ulrich Trappe Mareike Verbeek Daniel Wolff Rainer Blasczyk Britta Eiz-Vesper Britta Maecker-Kolhoff |
| author_facet | Agnes Bonifacius Britta Lamottke Sabine Tischer-Zimmermann Rebecca Schultze-Florey Lilia Goudeva Hans-Gert Heuft Lubomir Arseniev Rita Beier Gernot Beutel Gunnar Cario Birgit Fröhlich Johann Greil Leo Hansmann Justin Hasenkamp Michaela Höfs Patrick Hundsdoerfer Edgar Jost Kinan Kafa Oliver Kriege Nicolaus Kröger Stephan Mathas Roland Meisel Michaela Nathrath Mervi Putkonen Sarina Ravens Hans Christian Reinhardt Elisa Sala Martin G. Sauer Clemens Schmitt Roland Schroers Nina Kristin Steckel Ralf Ulrich Trappe Mareike Verbeek Daniel Wolff Rainer Blasczyk Britta Eiz-Vesper Britta Maecker-Kolhoff |
| author_sort | Agnes Bonifacius |
| collection | DOAJ |
| description | BACKGROUND Adoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODS We provide results of a personalized T cell manufacturing program evaluating donor, patient, T cell product, and outcome data. Patient-tailored clinical-grade EBV-specific cytotoxic T lymphocyte (EBV-CTL) products from stem cell donors (SCDs), related third-party donors (TPDs), or unrelated TPDs from the allogeneic T cell donor registry (alloCELL) at Hannover Medical School were manufactured by immunomagnetic selection using a CliniMACS Plus or Prodigy device and the EBV PepTivators EBNA-1 and Select. Consecutive manufacturing processes were evaluated, and patient outcome and side effects were retrieved by retrospective chart analysis.RESULTS Forty clinical-grade EBV-CTL products from SCDs, related TPDs, or unrelated TPDs were generated for 37 patients with refractory EBV infections or EBV-associated malignancies with and without a history of transplantation, within 5 days (median) after donor identification. Thirty-four patients received 1–14 EBV-CTL products (fresh and cryopreserved). EBV-CTL transfer led to a complete response in 20 of 29 patients who were evaluated for clinical response. No infusion-related toxicity was reported. EBV-specific T cells in patients’ blood were detectable in 16 of 18 monitored patients (89%) after transfer, and their presence correlated with clinical response.CONCLUSION Personalized clinical-grade manufacture of EBV-CTL products via immunomagnetic selection from SCDs, related TPDs, or unrelated TPDs in a timely manner is feasible. Overall, EBV-CTLs were clinically effective and well tolerated. Our data suggest EBV-CTL transfer as a promising therapeutic approach for immunocompromised patients with refractory EBV-associated diseases beyond HSCT, as well as patients with preexisting organ dysfunction.TRIAL REGISTRATION Not applicable.FUNDING This study was funded in part by the German Research Foundation (DFG, 158989968/SFB 900), the Deutsche Kinderkrebsstiftung (DKS 2013.09), Wilhelm-Sander-Stiftung (reference 2015.097.1), Ellen-Schmidt-Program of Hannover Medical School, and German Federal Ministry of Education and Research (reference 01EO0802). |
| first_indexed | 2024-03-11T12:08:57Z |
| format | Article |
| id | doaj.art-90b7781e7f1547e9ab2df6d697ea2d7d |
| institution | Directory Open Access Journal |
| issn | 1558-8238 |
| language | English |
| last_indexed | 2024-03-11T12:08:57Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Clinical Investigation |
| record_format | Article |
| series | The Journal of Clinical Investigation |
| spelling | doaj.art-90b7781e7f1547e9ab2df6d697ea2d7d2023-11-07T16:20:29ZengAmerican Society for Clinical InvestigationThe Journal of Clinical Investigation1558-82382023-06-0113312Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donorsAgnes BonifaciusBritta LamottkeSabine Tischer-ZimmermannRebecca Schultze-FloreyLilia GoudevaHans-Gert HeuftLubomir ArsenievRita BeierGernot BeutelGunnar CarioBirgit FröhlichJohann GreilLeo HansmannJustin HasenkampMichaela HöfsPatrick HundsdoerferEdgar JostKinan KafaOliver KriegeNicolaus KrögerStephan MathasRoland MeiselMichaela NathrathMervi PutkonenSarina RavensHans Christian ReinhardtElisa SalaMartin G. SauerClemens SchmittRoland SchroersNina Kristin SteckelRalf Ulrich TrappeMareike VerbeekDaniel WolffRainer BlasczykBritta Eiz-VesperBritta Maecker-KolhoffBACKGROUND Adoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODS We provide results of a personalized T cell manufacturing program evaluating donor, patient, T cell product, and outcome data. Patient-tailored clinical-grade EBV-specific cytotoxic T lymphocyte (EBV-CTL) products from stem cell donors (SCDs), related third-party donors (TPDs), or unrelated TPDs from the allogeneic T cell donor registry (alloCELL) at Hannover Medical School were manufactured by immunomagnetic selection using a CliniMACS Plus or Prodigy device and the EBV PepTivators EBNA-1 and Select. Consecutive manufacturing processes were evaluated, and patient outcome and side effects were retrieved by retrospective chart analysis.RESULTS Forty clinical-grade EBV-CTL products from SCDs, related TPDs, or unrelated TPDs were generated for 37 patients with refractory EBV infections or EBV-associated malignancies with and without a history of transplantation, within 5 days (median) after donor identification. Thirty-four patients received 1–14 EBV-CTL products (fresh and cryopreserved). EBV-CTL transfer led to a complete response in 20 of 29 patients who were evaluated for clinical response. No infusion-related toxicity was reported. EBV-specific T cells in patients’ blood were detectable in 16 of 18 monitored patients (89%) after transfer, and their presence correlated with clinical response.CONCLUSION Personalized clinical-grade manufacture of EBV-CTL products via immunomagnetic selection from SCDs, related TPDs, or unrelated TPDs in a timely manner is feasible. Overall, EBV-CTLs were clinically effective and well tolerated. Our data suggest EBV-CTL transfer as a promising therapeutic approach for immunocompromised patients with refractory EBV-associated diseases beyond HSCT, as well as patients with preexisting organ dysfunction.TRIAL REGISTRATION Not applicable.FUNDING This study was funded in part by the German Research Foundation (DFG, 158989968/SFB 900), the Deutsche Kinderkrebsstiftung (DKS 2013.09), Wilhelm-Sander-Stiftung (reference 2015.097.1), Ellen-Schmidt-Program of Hannover Medical School, and German Federal Ministry of Education and Research (reference 01EO0802).https://doi.org/10.1172/JCI163548Therapeutics |
| spellingShingle | Agnes Bonifacius Britta Lamottke Sabine Tischer-Zimmermann Rebecca Schultze-Florey Lilia Goudeva Hans-Gert Heuft Lubomir Arseniev Rita Beier Gernot Beutel Gunnar Cario Birgit Fröhlich Johann Greil Leo Hansmann Justin Hasenkamp Michaela Höfs Patrick Hundsdoerfer Edgar Jost Kinan Kafa Oliver Kriege Nicolaus Kröger Stephan Mathas Roland Meisel Michaela Nathrath Mervi Putkonen Sarina Ravens Hans Christian Reinhardt Elisa Sala Martin G. Sauer Clemens Schmitt Roland Schroers Nina Kristin Steckel Ralf Ulrich Trappe Mareike Verbeek Daniel Wolff Rainer Blasczyk Britta Eiz-Vesper Britta Maecker-Kolhoff Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors The Journal of Clinical Investigation Therapeutics |
| title | Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors |
| title_full | Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors |
| title_fullStr | Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors |
| title_full_unstemmed | Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors |
| title_short | Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors |
| title_sort | patient tailored adoptive immunotherapy with ebv specific t cells from related and unrelated donors |
| topic | Therapeutics |
| url | https://doi.org/10.1172/JCI163548 |
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