Preliminary study on the involvement of platelets in mouse experimental periodontitis
Background/purpose: Although some studies have taken an interest in the participation of platelets in periodontitis, so far, we know very little about the roles of platelets in periodontitis. The objective of this study is to explore the involvement of platelets in the development of experimental pe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S199179022200085X |
| _version_ | 1798030943659753472 |
|---|---|
| author | Jie Zhang Xian'e Wang Ruifang Lu Peihui Zou Yalin Zhan Huanxin Meng |
| author_facet | Jie Zhang Xian'e Wang Ruifang Lu Peihui Zou Yalin Zhan Huanxin Meng |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | Background/purpose: Although some studies have taken an interest in the participation of platelets in periodontitis, so far, we know very little about the roles of platelets in periodontitis. The objective of this study is to explore the involvement of platelets in the development of experimental periodontitis in mice. Materials and methods: Twenty C57BL/6 male mice were used for this study. Experimental periodontitis models of mice were constructed by ligating for 1, 3, 7, and 14 days, respectively. Morphological changes in the alveolar bone were assessed by micro-computed tomography (Micro-CT). The gingival crevicular fluid samples of ligation sites were collected and stained by immunocytochemistry. Immunohistochemistry was used to detect platelets infiltration in gingival tissues of mice. Results: The results of Micro-CT showed that with the extension of ligation time, alveolar bone resorption increased, suggesting that the experimental periodontitis models were established. Immunochemical staining showed that there were almost no platelets in the gingival crevicular fluid of mice ligated for 1 and 3 days. And at 7 and 14 days of ligation, a large number of platelets were present in the gingival crevicular fluid and formed complexes with neutrophils. And with the extension of ligation time, the extent of platelet infiltration increased in mice gingival tissues. Conclusion: Platelets were infiltrated increasedly in the gingival sulcus and gingival tissues following the experimental time, and may participate in the development of mouse experimental periodontitis. |
| first_indexed | 2024-04-11T19:49:27Z |
| format | Article |
| id | doaj.art-90b8f182c7a74a2d999ce4427fb7b8f4 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-11T19:49:27Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-90b8f182c7a74a2d999ce4427fb7b8f42022-12-22T04:06:22ZengElsevierJournal of Dental Sciences1991-79022022-10-0117414941500Preliminary study on the involvement of platelets in mouse experimental periodontitisJie Zhang0Xian'e Wang1Ruifang Lu2Peihui Zou3Yalin Zhan4Huanxin Meng5Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR ChinaDepartment of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR ChinaDepartment of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR ChinaDepartment of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR ChinaDepartment of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, PR China; Corresponding author. First Clinical Division, Peking University School and Hospital of Stomatology, 37A Xishiku Street, Xicheng District, Beijing, 100034, PR China.Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, PR China; National Center of Stomatology & National Clinical Research Center for Oral Diseases, Beijing, PR China; National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China; Corresponding author. Department of Periodontology, Peking University School and Hospital of Stomatology, No.22, Zhongguancun South Avenue, Haidian District, Beijing, 100081, PR China.Background/purpose: Although some studies have taken an interest in the participation of platelets in periodontitis, so far, we know very little about the roles of platelets in periodontitis. The objective of this study is to explore the involvement of platelets in the development of experimental periodontitis in mice. Materials and methods: Twenty C57BL/6 male mice were used for this study. Experimental periodontitis models of mice were constructed by ligating for 1, 3, 7, and 14 days, respectively. Morphological changes in the alveolar bone were assessed by micro-computed tomography (Micro-CT). The gingival crevicular fluid samples of ligation sites were collected and stained by immunocytochemistry. Immunohistochemistry was used to detect platelets infiltration in gingival tissues of mice. Results: The results of Micro-CT showed that with the extension of ligation time, alveolar bone resorption increased, suggesting that the experimental periodontitis models were established. Immunochemical staining showed that there were almost no platelets in the gingival crevicular fluid of mice ligated for 1 and 3 days. And at 7 and 14 days of ligation, a large number of platelets were present in the gingival crevicular fluid and formed complexes with neutrophils. And with the extension of ligation time, the extent of platelet infiltration increased in mice gingival tissues. Conclusion: Platelets were infiltrated increasedly in the gingival sulcus and gingival tissues following the experimental time, and may participate in the development of mouse experimental periodontitis.http://www.sciencedirect.com/science/article/pii/S199179022200085XPlateletsExperimental periodontitisGingival crevicular fluidInflammationGingival tissue |
| spellingShingle | Jie Zhang Xian'e Wang Ruifang Lu Peihui Zou Yalin Zhan Huanxin Meng Preliminary study on the involvement of platelets in mouse experimental periodontitis Journal of Dental Sciences Platelets Experimental periodontitis Gingival crevicular fluid Inflammation Gingival tissue |
| title | Preliminary study on the involvement of platelets in mouse experimental periodontitis |
| title_full | Preliminary study on the involvement of platelets in mouse experimental periodontitis |
| title_fullStr | Preliminary study on the involvement of platelets in mouse experimental periodontitis |
| title_full_unstemmed | Preliminary study on the involvement of platelets in mouse experimental periodontitis |
| title_short | Preliminary study on the involvement of platelets in mouse experimental periodontitis |
| title_sort | preliminary study on the involvement of platelets in mouse experimental periodontitis |
| topic | Platelets Experimental periodontitis Gingival crevicular fluid Inflammation Gingival tissue |
| url | http://www.sciencedirect.com/science/article/pii/S199179022200085X |
| work_keys_str_mv | AT jiezhang preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis AT xianewang preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis AT ruifanglu preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis AT peihuizou preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis AT yalinzhan preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis AT huanxinmeng preliminarystudyontheinvolvementofplateletsinmouseexperimentalperiodontitis |