Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1
Bacteriophages represent a valuable source for studying the mechanisms underlying virus-host interactions. A better understanding of the host-specificity of viruses at the molecular level can promote various phage applications, including bacterial diagnostics, antimicrobial therapeutics, and improve...
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| Format: | Article |
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MDPI AG
2015-11-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/12/2932 |
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| author | Eugenijus Šimoliūnas Monika Vilkaitytė Laura Kaliniene Aurelija Zajančkauskaitė Algirdas Kaupinis Juozas Staniulis Mindaugas Valius Rolandas Meškys Lidija Truncaitė |
| author_facet | Eugenijus Šimoliūnas Monika Vilkaitytė Laura Kaliniene Aurelija Zajančkauskaitė Algirdas Kaupinis Juozas Staniulis Mindaugas Valius Rolandas Meškys Lidija Truncaitė |
| author_sort | Eugenijus Šimoliūnas |
| collection | DOAJ |
| description | Bacteriophages represent a valuable source for studying the mechanisms underlying virus-host interactions. A better understanding of the host-specificity of viruses at the molecular level can promote various phage applications, including bacterial diagnostics, antimicrobial therapeutics, and improve methods in molecular biology. In this study, we describe the isolation and characterization of a novel coliphage, vB_EcoM_VpaE1, which has different host specificity than its relatives. Morphology studies, coupled with the results of genomic and proteomic analyses, indicate that vB_EcoM_VpaE1 belongs to the newly proposed genus Felix01likevirus in the family Myoviridae. The genus Felix01likevirus comprises a group of highly similar phages that infect O-antigen-expressing Salmonella and Escherichia coli (E. coli) strains. Phage vB_EcoM_VpaE1 differs from the rest of Felix01-like viruses, since it infects O-antigen-deficient E. coli strains with an incomplete core lipopolysaccharide (LPS). We show that vB_EcoM_VpaE1 can infect mutants of E. coli that contain various truncations in their LPS, and can even recognize LPS that is truncated down to the inner-core oligosaccharide, showing potential for the control of rough E. coli strains, which usually emerge as resistant mutants upon infection by O-Ag-specific phages. Furthermore, VpaE1 can replicate in a wide temperature range from 9 to 49 °C, suggesting that this virus is well adapted to harsh environmental conditions. Since the structural proteins of such phages tend to be rather robust, the receptor-recognizing proteins of VpaE1 are an attractive tool for application in glycan analysis, bacterial diagnostics and antimicrobial therapeutics. |
| first_indexed | 2024-12-14T13:33:24Z |
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| id | doaj.art-90b9ca26e5e7492ba5d50aabfedaaee1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-14T13:33:24Z |
| publishDate | 2015-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-90b9ca26e5e7492ba5d50aabfedaaee12022-12-21T22:59:38ZengMDPI AGViruses1999-49152015-11-017126163618110.3390/v7122932v7122932Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1Eugenijus Šimoliūnas0Monika Vilkaitytė1Laura Kaliniene2Aurelija Zajančkauskaitė3Algirdas Kaupinis4Juozas Staniulis5Mindaugas Valius6Rolandas Meškys7Lidija Truncaitė8Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaProteomics Centre, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaLaboratory of Plant Viruses, Institute of Botany, Nature Research Centre, Vilnius LT-08412, LithuaniaProteomics Centre, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius LT-08662, LithuaniaBacteriophages represent a valuable source for studying the mechanisms underlying virus-host interactions. A better understanding of the host-specificity of viruses at the molecular level can promote various phage applications, including bacterial diagnostics, antimicrobial therapeutics, and improve methods in molecular biology. In this study, we describe the isolation and characterization of a novel coliphage, vB_EcoM_VpaE1, which has different host specificity than its relatives. Morphology studies, coupled with the results of genomic and proteomic analyses, indicate that vB_EcoM_VpaE1 belongs to the newly proposed genus Felix01likevirus in the family Myoviridae. The genus Felix01likevirus comprises a group of highly similar phages that infect O-antigen-expressing Salmonella and Escherichia coli (E. coli) strains. Phage vB_EcoM_VpaE1 differs from the rest of Felix01-like viruses, since it infects O-antigen-deficient E. coli strains with an incomplete core lipopolysaccharide (LPS). We show that vB_EcoM_VpaE1 can infect mutants of E. coli that contain various truncations in their LPS, and can even recognize LPS that is truncated down to the inner-core oligosaccharide, showing potential for the control of rough E. coli strains, which usually emerge as resistant mutants upon infection by O-Ag-specific phages. Furthermore, VpaE1 can replicate in a wide temperature range from 9 to 49 °C, suggesting that this virus is well adapted to harsh environmental conditions. Since the structural proteins of such phages tend to be rather robust, the receptor-recognizing proteins of VpaE1 are an attractive tool for application in glycan analysis, bacterial diagnostics and antimicrobial therapeutics.http://www.mdpi.com/1999-4915/7/12/2932E. coli lipopolysaccharideinner core oligosacharidebacteriophage vB_EcoM_VpaE1 host-rangegenus Felix01likevirusJ0101 |
| spellingShingle | Eugenijus Šimoliūnas Monika Vilkaitytė Laura Kaliniene Aurelija Zajančkauskaitė Algirdas Kaupinis Juozas Staniulis Mindaugas Valius Rolandas Meškys Lidija Truncaitė Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 Viruses E. coli lipopolysaccharide inner core oligosacharide bacteriophage vB_EcoM_VpaE1 host-range genus Felix01likevirus J0101 |
| title | Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 |
| title_full | Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 |
| title_fullStr | Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 |
| title_full_unstemmed | Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 |
| title_short | Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1 |
| title_sort | incomplete lps core specific felix01 like virus vb ecom vpae1 |
| topic | E. coli lipopolysaccharide inner core oligosacharide bacteriophage vB_EcoM_VpaE1 host-range genus Felix01likevirus J0101 |
| url | http://www.mdpi.com/1999-4915/7/12/2932 |
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