Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland
The increasing population of European bison (Bison bonasus) can contribute to the prevalence of zoonotic pathogens. The aim of the present study was to assess the presence of A. phagocytophilum infection in European bison tissues as well as ticks removed from European bison in Lithuania and Poland....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Ticks and Tick-Borne Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X23001279 |
| _version_ | 1797676300861702144 |
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| author | Indrė Lipatova Dalia Černevičienė Loreta Griciuvienė Irma Ražanskė Asta Aleksandravičienė Artūras Kibiša Jana Radzijevskaja Wanda Olech Krzysztof Anusz Anna Didkowska Algimantas Paulauskas |
| author_facet | Indrė Lipatova Dalia Černevičienė Loreta Griciuvienė Irma Ražanskė Asta Aleksandravičienė Artūras Kibiša Jana Radzijevskaja Wanda Olech Krzysztof Anusz Anna Didkowska Algimantas Paulauskas |
| author_sort | Indrė Lipatova |
| collection | DOAJ |
| description | The increasing population of European bison (Bison bonasus) can contribute to the prevalence of zoonotic pathogens. The aim of the present study was to assess the presence of A. phagocytophilum infection in European bison tissues as well as ticks removed from European bison in Lithuania and Poland. A further objective of this work was to compare the detected A. phagocytophilum strains. A total of 85 tissue samples (spleen) of European bison and 560 ticks belonging to two species, Ixodes ricinus (n = 408) and Dermacentor reticulatus (n = 152) were tested. DNA of A. phagocytophilum was detected based on RT-PCR in 40% of the European bison samples, 8.8% of the I. ricinus and 5.9% of the D. reticulatus ticks. Analysis of the obtained partial 16S rRNA gene sequences of A. phagocytophilum revealed the presence of three variants with two polymorphic sites. Furthermore, phylogenetic analysis with partial msp4 gene sequences grouped A. phagocytophilum variants into three clusters. This study revealed that the groEL gene sequences of A. phagocytophilum from European bison and their ticks grouped into ecotype I and only one sequence from Lithuanian European bison belonged to ecotype II. The results of the present study indicated that European bison may play a role as a natural reservoir of A. phagocytophilum. |
| first_indexed | 2024-03-11T22:27:00Z |
| format | Article |
| id | doaj.art-90bc9fec6bad4cf39f109933b65f776b |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-03-11T22:27:00Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-90bc9fec6bad4cf39f109933b65f776b2023-09-24T05:14:40ZengElsevierTicks and Tick-Borne Diseases1877-96032023-11-01146102246Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and PolandIndrė Lipatova0Dalia Černevičienė1Loreta Griciuvienė2Irma Ražanskė3Asta Aleksandravičienė4Artūras Kibiša5Jana Radzijevskaja6Wanda Olech7Krzysztof Anusz8Anna Didkowska9Algimantas Paulauskas10Vytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, Lithuania; Corresponding author at. Indrė Lipatova, Vytautas Magnus University, Department of Biology, Universiteto 10, LT-53361, Akademija, Kaunas District, Lithuania.Vytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaDepartment of Animal Genetics and Conservation, Warsaw University of Life Sciences (SGGW), Ciszewskiego 8, 02-786 Warsaw, PolandDepartment of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, PolandDepartment of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, PolandVytautas Magnus University, K. Donelaičio 58, Kaunas LT-44248, LithuaniaThe increasing population of European bison (Bison bonasus) can contribute to the prevalence of zoonotic pathogens. The aim of the present study was to assess the presence of A. phagocytophilum infection in European bison tissues as well as ticks removed from European bison in Lithuania and Poland. A further objective of this work was to compare the detected A. phagocytophilum strains. A total of 85 tissue samples (spleen) of European bison and 560 ticks belonging to two species, Ixodes ricinus (n = 408) and Dermacentor reticulatus (n = 152) were tested. DNA of A. phagocytophilum was detected based on RT-PCR in 40% of the European bison samples, 8.8% of the I. ricinus and 5.9% of the D. reticulatus ticks. Analysis of the obtained partial 16S rRNA gene sequences of A. phagocytophilum revealed the presence of three variants with two polymorphic sites. Furthermore, phylogenetic analysis with partial msp4 gene sequences grouped A. phagocytophilum variants into three clusters. This study revealed that the groEL gene sequences of A. phagocytophilum from European bison and their ticks grouped into ecotype I and only one sequence from Lithuanian European bison belonged to ecotype II. The results of the present study indicated that European bison may play a role as a natural reservoir of A. phagocytophilum.http://www.sciencedirect.com/science/article/pii/S1877959X23001279AnaplasmaBison bonasusIxodes ricinusDermacentor reticulatus |
| spellingShingle | Indrė Lipatova Dalia Černevičienė Loreta Griciuvienė Irma Ražanskė Asta Aleksandravičienė Artūras Kibiša Jana Radzijevskaja Wanda Olech Krzysztof Anusz Anna Didkowska Algimantas Paulauskas Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland Ticks and Tick-Borne Diseases Anaplasma Bison bonasus Ixodes ricinus Dermacentor reticulatus |
| title | Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland |
| title_full | Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland |
| title_fullStr | Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland |
| title_full_unstemmed | Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland |
| title_short | Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland |
| title_sort | anaplasma phagocytophilum in european bison bison bonasus and their ticks from lithuania and poland |
| topic | Anaplasma Bison bonasus Ixodes ricinus Dermacentor reticulatus |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X23001279 |
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