Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV)
Introduction. The mouse-specific orthopoxvirus, ectromelia virus, is one of the best models that can be used to study key issues of pathogenesis, prevention, and treatment of smallpox, and to develop measures to increase virulence, transmissibility, or the ability to overcome vaccine immunity. Th...
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| Format: | Article |
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Central Research Institute for Epidemiology
2023-09-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/10570/795 |
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| author | Tamara V. Teplyakova Alexey S. Kabanov Alena S. Ovchinnikova Dmitry A. Odnoshevskyh Irina F. Petrovskaya Tatiana S. Nepomnyashchikh Oleg V. Pyankov |
| author_facet | Tamara V. Teplyakova Alexey S. Kabanov Alena S. Ovchinnikova Dmitry A. Odnoshevskyh Irina F. Petrovskaya Tatiana S. Nepomnyashchikh Oleg V. Pyankov |
| author_sort | Tamara V. Teplyakova |
| collection | DOAJ |
| description | Introduction. The mouse-specific orthopoxvirus, ectromelia virus, is one of the best models that can be used to study key issues of pathogenesis, prevention, and treatment of smallpox, and to develop measures to increase virulence, transmissibility, or the ability to overcome vaccine immunity.
The aim of the work is to screen the antiviral activity of samples from Inonotus obliquus chaga and humic acid from brown coal in vitro against ectromelia virus.
Materials and methods. We used ectromelia virus, strain K-1 (reg. No V-142), obtained from the State Collection of Pathogens of Viral Infections and Rickettsioses of the State Scientific Center of Virology and Biotechnology Vector; Vero Е6 cell culture (No 70) from the Collection of cell cultures of the State Scientific Center of Virology and Biotechnology Vector. Nine samples from chaga I. obliquus and humic acid from brown coal were used to evaluate the changes in the infectivity of the ectromelia virus on cell culture using 2 schemes of application of drugs and virus (preventive and therapeutic schemes), and to assess their cytotoxicity and antiviral activity.
Results. 50% cytotoxic concentration, 50% virus-inhibiting concentrations and selectivity index were determined for all samples. The studied samples were shown to be non-toxic to the monolayer of Vero cell culture in a dilution of 300 and more micrograms/ml, while demonstrated high antiviral activity against strain K-1 of ectromelia virus in two application schemes preventive and curative.
Conclusion. All samples tested for ectromelia virus in vitro can be considered promising for further development of drugs against diseases caused by orthopoxviruses. |
| first_indexed | 2024-03-11T10:51:10Z |
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| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-11T10:51:10Z |
| publishDate | 2023-09-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-90be7552ab404c83abe87209f6eed99a2023-11-13T17:14:22ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972023-09-0168427728210.36233/0507-4088-1741174Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV)Tamara V. Teplyakova0https://orcid.org/0000-0002-0621-8698Alexey S. Kabanov1https://orcid.org/0000-0002-6287-0912Alena S. Ovchinnikova2https://orcid.org/0000-0002-1745-7643Dmitry A. Odnoshevskyh3https://orcid.org/0000-0003-0616-7488Irina F. Petrovskaya4https://orcid.org/0000-0002-9833-0555Tatiana S. Nepomnyashchikh5https://orcid.org/0000-0003-0592-8273Oleg V. Pyankov6State Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorState Research Center of Virology and Biotechnology “Vector” of RospotrebnadzorIntroduction. The mouse-specific orthopoxvirus, ectromelia virus, is one of the best models that can be used to study key issues of pathogenesis, prevention, and treatment of smallpox, and to develop measures to increase virulence, transmissibility, or the ability to overcome vaccine immunity. The aim of the work is to screen the antiviral activity of samples from Inonotus obliquus chaga and humic acid from brown coal in vitro against ectromelia virus. Materials and methods. We used ectromelia virus, strain K-1 (reg. No V-142), obtained from the State Collection of Pathogens of Viral Infections and Rickettsioses of the State Scientific Center of Virology and Biotechnology Vector; Vero Е6 cell culture (No 70) from the Collection of cell cultures of the State Scientific Center of Virology and Biotechnology Vector. Nine samples from chaga I. obliquus and humic acid from brown coal were used to evaluate the changes in the infectivity of the ectromelia virus on cell culture using 2 schemes of application of drugs and virus (preventive and therapeutic schemes), and to assess their cytotoxicity and antiviral activity. Results. 50% cytotoxic concentration, 50% virus-inhibiting concentrations and selectivity index were determined for all samples. The studied samples were shown to be non-toxic to the monolayer of Vero cell culture in a dilution of 300 and more micrograms/ml, while demonstrated high antiviral activity against strain K-1 of ectromelia virus in two application schemes preventive and curative. Conclusion. All samples tested for ectromelia virus in vitro can be considered promising for further development of drugs against diseases caused by orthopoxviruses.https://virusjour.crie.ru/jour/article/viewFile/10570/795chagainonotus obliquusmelaninhumic acidectromelia virusin vitroantiviral activity |
| spellingShingle | Tamara V. Teplyakova Alexey S. Kabanov Alena S. Ovchinnikova Dmitry A. Odnoshevskyh Irina F. Petrovskaya Tatiana S. Nepomnyashchikh Oleg V. Pyankov Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) Вопросы вирусологии chaga inonotus obliquus melanin humic acid ectromelia virus in vitro antiviral activity |
| title | Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) |
| title_full | Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) |
| title_fullStr | Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) |
| title_full_unstemmed | Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) |
| title_short | Screening of antiviral activity of samples from chaga <i>Inonotus obliquus</i> and humic acid from brown coal on <i>Vero</i> cell culture against ectromelia virus (Poxviridae: <i>Orthopoxvirus</i>; ECTV) |
| title_sort | screening of antiviral activity of samples from chaga i inonotus obliquus i and humic acid from brown coal on i vero i cell culture against ectromelia virus poxviridae i orthopoxvirus i ectv |
| topic | chaga inonotus obliquus melanin humic acid ectromelia virus in vitro antiviral activity |
| url | https://virusjour.crie.ru/jour/article/viewFile/10570/795 |
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