Possibilities of using a miniature fluorescence microscope
The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf |
| _version_ | 1818718264847499264 |
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| author | Erofeev Alexander Gerasimov Evgeny Barinov Dmitry Bolsunovskaya Marina Bezprozvanny Ilya |
| author_facet | Erofeev Alexander Gerasimov Evgeny Barinov Dmitry Bolsunovskaya Marina Bezprozvanny Ilya |
| author_sort | Erofeev Alexander |
| collection | DOAJ |
| description | The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions. |
| first_indexed | 2024-12-17T19:48:17Z |
| format | Article |
| id | doaj.art-90c0ba7523164506ba70d4a61307db28 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-17T19:48:17Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-90c0ba7523164506ba70d4a61307db282022-12-21T21:34:48ZengEDP SciencesE3S Web of Conferences2267-12422020-01-012240200510.1051/e3sconf/202022402005e3sconf_TPACEE2020_02005Possibilities of using a miniature fluorescence microscopeErofeev Alexander0Gerasimov Evgeny1Barinov Dmitry2Bolsunovskaya Marina3Bezprozvanny IlyaPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityThe article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf |
| spellingShingle | Erofeev Alexander Gerasimov Evgeny Barinov Dmitry Bolsunovskaya Marina Bezprozvanny Ilya Possibilities of using a miniature fluorescence microscope E3S Web of Conferences |
| title | Possibilities of using a miniature fluorescence microscope |
| title_full | Possibilities of using a miniature fluorescence microscope |
| title_fullStr | Possibilities of using a miniature fluorescence microscope |
| title_full_unstemmed | Possibilities of using a miniature fluorescence microscope |
| title_short | Possibilities of using a miniature fluorescence microscope |
| title_sort | possibilities of using a miniature fluorescence microscope |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf |
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