Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers
In the present study, the underlying short-term effects of gold nanoparticles (AuNP, spheres, citrate coated, ∼40 nm) on the hepatic function of gilthead sea bream (Sparus aurata) was assessed, using a species-specific enriched oligonucleotide microarray platform (SAQ). Two distinct concentrations o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmars.2019.00147/full |
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| author | Mariana Teles Felipe E. Reyes-López Joan C. Balasch Asta Tvarijonaviciute Laura Guimarães Miguel Oliveira Lluis Tort |
| author_facet | Mariana Teles Felipe E. Reyes-López Joan C. Balasch Asta Tvarijonaviciute Laura Guimarães Miguel Oliveira Lluis Tort |
| author_sort | Mariana Teles |
| collection | DOAJ |
| description | In the present study, the underlying short-term effects of gold nanoparticles (AuNP, spheres, citrate coated, ∼40 nm) on the hepatic function of gilthead sea bream (Sparus aurata) was assessed, using a species-specific enriched oligonucleotide microarray platform (SAQ). Two distinct concentrations of AuNP (0.5 and 50 μg/L) were tested during 24 h waterborne exposure. The transcriptional profile was complemented with outcomes at higher levels of biological organization, including hepatic health indicators and DNA damage indicators. DNA damaging potential of AuNP was assessed in whole peripheral blood, assessing DNA strand breaks (using the comet assay) and chromosome damage (scoring the erythrocytic nuclear abnormalities, ENA). The overall genetic response showed a differential hepatic transcriptional profile, both in terms of number and intensity, of differentially expressed genes (DEG). Concerning the functional pathways that were affected, the main changes found were for gene encoding proteins involved in the response to xenobiotics, oxidoreductase activity, immunomodulation, DNA repair and programmed cell death types I and II. The hepatic health indicators measured revealed that AuNP can induce liver injury, as demonstrated by the plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly increased activities after exposure to the highest AuNP concentration (50 μg/L). Exposure to AuNP also caused DNA strand breaks, however, without causing clastogenesis or aneugenesis, since no ENA were detected. Overall, data showed that a short-term exposure to AuNP can modulate gene expression in liver and affects several biochemical/genetic functions in fish. |
| first_indexed | 2024-12-11T06:26:03Z |
| format | Article |
| id | doaj.art-90c148ed37c945ee829ab1eebf59bf73 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-11T06:26:03Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-90c148ed37c945ee829ab1eebf59bf732022-12-22T01:17:40ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452019-04-01610.3389/fmars.2019.00147432922Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical BiomarkersMariana Teles0Felipe E. Reyes-López1Joan C. Balasch2Asta Tvarijonaviciute3Laura Guimarães4Miguel Oliveira5Lluis Tort6CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, Matosinhos, PortugalDepartment of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Barcelona, SpainDepartment of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Barcelona, SpainInterdisciplinary Laboratory of Clinical Analysis INTERLAB-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, SpainCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, Matosinhos, PortugalDepartment of Biology and CESAM, University of Aveiro, Aveiro, PortugalDepartment of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Barcelona, SpainIn the present study, the underlying short-term effects of gold nanoparticles (AuNP, spheres, citrate coated, ∼40 nm) on the hepatic function of gilthead sea bream (Sparus aurata) was assessed, using a species-specific enriched oligonucleotide microarray platform (SAQ). Two distinct concentrations of AuNP (0.5 and 50 μg/L) were tested during 24 h waterborne exposure. The transcriptional profile was complemented with outcomes at higher levels of biological organization, including hepatic health indicators and DNA damage indicators. DNA damaging potential of AuNP was assessed in whole peripheral blood, assessing DNA strand breaks (using the comet assay) and chromosome damage (scoring the erythrocytic nuclear abnormalities, ENA). The overall genetic response showed a differential hepatic transcriptional profile, both in terms of number and intensity, of differentially expressed genes (DEG). Concerning the functional pathways that were affected, the main changes found were for gene encoding proteins involved in the response to xenobiotics, oxidoreductase activity, immunomodulation, DNA repair and programmed cell death types I and II. The hepatic health indicators measured revealed that AuNP can induce liver injury, as demonstrated by the plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly increased activities after exposure to the highest AuNP concentration (50 μg/L). Exposure to AuNP also caused DNA strand breaks, however, without causing clastogenesis or aneugenesis, since no ENA were detected. Overall, data showed that a short-term exposure to AuNP can modulate gene expression in liver and affects several biochemical/genetic functions in fish.https://www.frontiersin.org/article/10.3389/fmars.2019.00147/fullnanotoxicologymicroarraysgenotoxicitySparus aurataenvironmental monitoring |
| spellingShingle | Mariana Teles Felipe E. Reyes-López Joan C. Balasch Asta Tvarijonaviciute Laura Guimarães Miguel Oliveira Lluis Tort Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers Frontiers in Marine Science nanotoxicology microarrays genotoxicity Sparus aurata environmental monitoring |
| title | Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers |
| title_full | Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers |
| title_fullStr | Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers |
| title_full_unstemmed | Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers |
| title_short | Toxicogenomics of Gold Nanoparticles in a Marine Fish: Linkage to Classical Biomarkers |
| title_sort | toxicogenomics of gold nanoparticles in a marine fish linkage to classical biomarkers |
| topic | nanotoxicology microarrays genotoxicity Sparus aurata environmental monitoring |
| url | https://www.frontiersin.org/article/10.3389/fmars.2019.00147/full |
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