DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate
The aim of this study was to elucidate the role of DNA methylation-silenced genes in malignant transformation. We screened DNA methylation-silenced genes during methacrylate (GMA)-induced malignant transformation of human bronchial epithelial cells (16HBE) by DNA methylation microarray analysis. The...
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| Format: | Article |
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Taylor & Francis Group
2023-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2023.2206543 |
| _version_ | 1826978978147074048 |
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| author | Quankai Wang Boshen Wang Huanhuan Li Miao Wang Xinwei Li Baoli Zhu Jianning Xu |
| author_facet | Quankai Wang Boshen Wang Huanhuan Li Miao Wang Xinwei Li Baoli Zhu Jianning Xu |
| author_sort | Quankai Wang |
| collection | DOAJ |
| description | The aim of this study was to elucidate the role of DNA methylation-silenced genes in malignant transformation. We screened DNA methylation-silenced genes during methacrylate (GMA)-induced malignant transformation of human bronchial epithelial cells (16HBE) by DNA methylation microarray analysis. The methylation status of some identified genes was assessed by a polymerase chain reaction and compared with uninduced control cells. A total of 801, 66 and 30 genes were silenced in 10th, 20th and 30th generation cells; 8 genes were silenced in 10th and 20th generation cells but not in 30th generation cells; 14 genes were silenced in 30th and 10th generation cells but not in 20th generation cells; and one gene was silenced in 20th and 30th generation cells but not in 10th generation cells. TRPV1 and SOD2 genes were methylated and their gene expression levels were significantly lower in 30th generation 16HBE cells treated with GMA compared to uninduced control cells (P < 0.05). The expression levels of DNA methylation-silenced genes were altered throughout the GMA-induced malignant transformation of 16HBE cells. Furthermore, TRPV1/SOD2 may represent a specific molecular marker associated with the malignant transformation of GMA-stimulated 16HBE cells. |
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| id | doaj.art-90c1beaa431748ee9fcb4b60875404b0 |
| institution | Directory Open Access Journal |
| issn | 2689-5307 |
| language | English |
| last_indexed | 2025-02-18T05:21:48Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-90c1beaa431748ee9fcb4b60875404b02024-11-15T10:36:02ZengTaylor & Francis GroupAll Life2689-53072023-12-01160110.1080/26895293.2023.22065432206543DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylateQuankai Wang0Boshen Wang1Huanhuan Li2Miao Wang3Xinwei Li4Baoli Zhu5Jianning Xu6National Institute of Occupational Health and Poison ControlNational Institute of Occupational Health and Poison ControlNational Institute of Occupational Health and Poison ControlNational Institute of Occupational Health and Poison ControlNational Institute of Occupational Health and Poison ControlJiangsu Center for Disease Control and Prevention, Nanjing, People’s Republic of ChinaNational Institute of Occupational Health and Poison ControlThe aim of this study was to elucidate the role of DNA methylation-silenced genes in malignant transformation. We screened DNA methylation-silenced genes during methacrylate (GMA)-induced malignant transformation of human bronchial epithelial cells (16HBE) by DNA methylation microarray analysis. The methylation status of some identified genes was assessed by a polymerase chain reaction and compared with uninduced control cells. A total of 801, 66 and 30 genes were silenced in 10th, 20th and 30th generation cells; 8 genes were silenced in 10th and 20th generation cells but not in 30th generation cells; 14 genes were silenced in 30th and 10th generation cells but not in 20th generation cells; and one gene was silenced in 20th and 30th generation cells but not in 10th generation cells. TRPV1 and SOD2 genes were methylated and their gene expression levels were significantly lower in 30th generation 16HBE cells treated with GMA compared to uninduced control cells (P < 0.05). The expression levels of DNA methylation-silenced genes were altered throughout the GMA-induced malignant transformation of 16HBE cells. Furthermore, TRPV1/SOD2 may represent a specific molecular marker associated with the malignant transformation of GMA-stimulated 16HBE cells.http://dx.doi.org/10.1080/26895293.2023.2206543dna methylationglycidyl methacrylate16hbe |
| spellingShingle | Quankai Wang Boshen Wang Huanhuan Li Miao Wang Xinwei Li Baoli Zhu Jianning Xu DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate All Life dna methylation glycidyl methacrylate 16hbe |
| title | DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| title_full | DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| title_fullStr | DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| title_full_unstemmed | DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| title_short | DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| title_sort | dna methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate |
| topic | dna methylation glycidyl methacrylate 16hbe |
| url | http://dx.doi.org/10.1080/26895293.2023.2206543 |
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