BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study
Arecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/10/14/5008 |
| _version_ | 1827712676673880064 |
|---|---|
| author | Deepti Shrivastava Kumar Chandan Srivastava Jithendra Kajoor Dayakara Mohammed G. Sghaireen Ravi Kumar Gudipaneni Khalid Al-Johani Muhammad Nadeem Baig Zohaib Khurshid |
| author_facet | Deepti Shrivastava Kumar Chandan Srivastava Jithendra Kajoor Dayakara Mohammed G. Sghaireen Ravi Kumar Gudipaneni Khalid Al-Johani Muhammad Nadeem Baig Zohaib Khurshid |
| author_sort | Deepti Shrivastava |
| collection | DOAJ |
| description | Arecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy subjects and chronic periodontitis (CP) patients. An in vitro study was designed with an equal number of (<i>n</i> = 30) gingival crevicular fluid (GCF) samples collected from CP patients and healthy subjects. Bactericidal activity and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) assays were performed with the GCF samples pre-treated with extracts of two varieties of areca nut: ripe and tender. Simultaneously, controls were also carried out with Hank’s balanced salt solution (HBSS) and catechin. Independent t-test and one-way analysis of variance (ANOVA), along with post-hoc analysis, were employed for statistical analysis. In both study groups, a significant reduction (<i>p</i> < 0.01)in the bactericidal activity was noted when the samples treated with the ripe areca nut (rANE) were compared with the tender variant (tANE). Similarly, H<sub>2</sub>O<sub>2</sub> levels were significantly reduced (<i>p</i> < 0.001) in the rANE in contrast to tANE for both study groups. The above results were significant within the group but were found to be non-significant between the study groups, except when it was treated with HBSS (<i>p</i> < 0.001). In the present study, it was found that there was a reduction in the bactericidal activity and H<sub>2</sub>O<sub>2</sub> production of cPMNs in both healthy subjects and CP patients in the presence of areca nut extract. Moreover, the effect of rANE on cPMNs was more detrimental than tANE. |
| first_indexed | 2024-03-10T18:19:57Z |
| format | Article |
| id | doaj.art-90c2a91675254d379ba764e4ef52f038 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T18:19:57Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-90c2a91675254d379ba764e4ef52f0382023-11-20T07:25:58ZengMDPI AGApplied Sciences2076-34172020-07-011014500810.3390/app10145008BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro StudyDeepti Shrivastava0Kumar Chandan Srivastava1Jithendra Kajoor Dayakara2Mohammed G. Sghaireen3Ravi Kumar Gudipaneni4Khalid Al-Johani5Muhammad Nadeem Baig6Zohaib Khurshid7Department of Preventive Dentistry, College of Dentistry, Jouf University, Sakakah 72345, Saudi ArabiaDepartment of Oral and Maxillofacial Surgery & Diagnostic Sciences, College of Dentistry, Jouf University, Sakakah 72345, Saudi ArabiaDepartment of Periodontics, Kantidevi Dental College, Mathura 281121, IndiaDepartment of Prosthetic Dental Sciences, College of Dentistry, Jouf University, Sakakah 72345, Saudi ArabiaDepartment of Preventive Dentistry, College of Dentistry, Jouf University, Sakakah 72345, Saudi ArabiaDepartment of Oral Diagnostic Sciences, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Preventive Dentistry, College of Dentistry, Jouf University, Sakakah 72345, Saudi ArabiaDepartment of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa31982, Saudi ArabiaArecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy subjects and chronic periodontitis (CP) patients. An in vitro study was designed with an equal number of (<i>n</i> = 30) gingival crevicular fluid (GCF) samples collected from CP patients and healthy subjects. Bactericidal activity and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) assays were performed with the GCF samples pre-treated with extracts of two varieties of areca nut: ripe and tender. Simultaneously, controls were also carried out with Hank’s balanced salt solution (HBSS) and catechin. Independent t-test and one-way analysis of variance (ANOVA), along with post-hoc analysis, were employed for statistical analysis. In both study groups, a significant reduction (<i>p</i> < 0.01)in the bactericidal activity was noted when the samples treated with the ripe areca nut (rANE) were compared with the tender variant (tANE). Similarly, H<sub>2</sub>O<sub>2</sub> levels were significantly reduced (<i>p</i> < 0.001) in the rANE in contrast to tANE for both study groups. The above results were significant within the group but were found to be non-significant between the study groups, except when it was treated with HBSS (<i>p</i> < 0.001). In the present study, it was found that there was a reduction in the bactericidal activity and H<sub>2</sub>O<sub>2</sub> production of cPMNs in both healthy subjects and CP patients in the presence of areca nut extract. Moreover, the effect of rANE on cPMNs was more detrimental than tANE.https://www.mdpi.com/2076-3417/10/14/5008neutrophilsareca nutperiodontitisantibacterial activityreactive oxygen species |
| spellingShingle | Deepti Shrivastava Kumar Chandan Srivastava Jithendra Kajoor Dayakara Mohammed G. Sghaireen Ravi Kumar Gudipaneni Khalid Al-Johani Muhammad Nadeem Baig Zohaib Khurshid BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study Applied Sciences neutrophils areca nut periodontitis antibacterial activity reactive oxygen species |
| title | BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study |
| title_full | BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study |
| title_fullStr | BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study |
| title_full_unstemmed | BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study |
| title_short | BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study |
| title_sort | bactericidalactivity of crevicular polymorphonuclear neutrophils in chronic periodontitis patients and healthy subjects under the influence of areca nut extract an in vitro study |
| topic | neutrophils areca nut periodontitis antibacterial activity reactive oxygen species |
| url | https://www.mdpi.com/2076-3417/10/14/5008 |
| work_keys_str_mv | AT deeptishrivastava bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT kumarchandansrivastava bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT jithendrakajoordayakara bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT mohammedgsghaireen bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT ravikumargudipaneni bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT khalidaljohani bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT muhammadnadeembaig bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy AT zohaibkhurshid bactericidalactivityofcrevicularpolymorphonuclearneutrophilsinchronicperiodontitispatientsandhealthysubjectsundertheinfluenceofarecanutextractaninvitrostudy |