Polymorphisms of the <i>BMPR1B</i>, <i>BMP15</i> and <i>GDF9</i> fecundity genes in four Chinese sheep breeds
<p>Numerous studies on prolific sheep breeds have shown that the transforming growth factor beta (TGF-<span class="inline-formula"><i>β</i></span>) superfamily members, including bone morphogenetic protein receptor type 1B (<i>BMPR1B</i>), bone mor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2024-02-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/67/51/2024/aab-67-51-2024.pdf |
| Summary: | <p>Numerous studies on prolific sheep breeds have shown that the transforming growth factor beta (TGF-<span class="inline-formula"><i>β</i></span>) superfamily members, including bone morphogenetic protein receptor type 1B (<i>BMPR1B</i>), bone morphogenetic protein 15 (<i>BMP15</i>) and growth differentiation factor 9 (<i>GDF9</i>), are the essential regulators of ovulation rate and litter size. In total, 11 known mutations (1 in <i>BMPR1B</i>, 6 in <i>BMP15</i> and 4 in <i>GDF9</i>) are able to increase the ovulation rate and litter size, respectively. In this study, the genomic DNA was isolated from 512 high-prolificacy sheep (including the Small-tailed Han, Hu and Wadi sheep breeds) and 164 low-prolificacy sheep (Tan sheep), and genotyping of the specific mutations of the three fecundity-related genes was carried out by sequencing. The results showed that the <i>FecB</i> mutation in <i>BMPR1B</i> was detected in all four sheep breeds, and the frequency of B allele was significantly higher in the high-prolificacy breeds than that in the low-prolificacy breed (<span class="inline-formula"><i>P</i><0.001</span>). A novel mutation, c.T755C (named S1), was found in <i>BMP15</i> from the four sheep breeds. However, known mutations such as <i>FecX</i><span class="inline-formula"><sup><i>I</i></sup></span>, <i>FecX</i><span class="inline-formula"><sup><i>H</i></sup></span>, <i>FecX</i><span class="inline-formula"><sup><i>B</i></sup></span>, <i>FecX</i><span class="inline-formula"><sup><i>G</i></sup></span>, <i>FecX</i><span class="inline-formula"><sup><i>L</i></sup></span> and <i>FecX</i><span class="inline-formula"><sup><i>R</i></sup></span> were not detected in these breeds. Three known loci (G1, G3 and G4) and a new mutation, c.A1515G (named S2), were found in <i>GDF9</i>, and the other three known mutations (<i>FecG</i><span class="inline-formula"><sup><i>H</i></sup></span> (G8), <i>FecG</i><span class="inline-formula"><sup><i>E</i></sup></span> and <i>FecTT</i>) were not detected in all four sheep breeds. The genotype distribution at the G1 and G4 loci had significant differences between the low-prolificacy sheep breed and the other three high-prolificacy sheep breeds. There was no difference in the genotype distribution at the G1 and G4 loci between the three high-prolificacy sheep breeds. Haplotype analysis of the four polymorphic loci in <i>GDF9</i> suggested that H4 (GGAA) was the preponderant haplotype in the three high-prolificacy sheep breeds, but H1 (GGGG) was in the low-prolificacy sheep breed. These results preliminarily showed that the <i>BMPR1B</i> and <i>GDF9</i> might be major genes influencing the prolificacy of Chinese sheep breeds.</p> |
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| ISSN: | 0003-9438 2363-9822 |