Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.
A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal-...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25014571/pdf/?tool=EBI |
| _version_ | 1819181047319887872 |
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| author | Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han |
| author_facet | Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han |
| author_sort | Xiao-Qiang Han |
| collection | DOAJ |
| description | A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells. |
| first_indexed | 2024-12-22T22:24:01Z |
| format | Article |
| id | doaj.art-90c75538544d4575aeff4ee04206414b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T22:24:01Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-90c75538544d4575aeff4ee04206414b2022-12-21T18:10:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10038010.1371/journal.pone.0100380Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.Xiao-Qiang HanGar-Lee YueRui-Qi YueCai-Xia DongChung-Lap ChanChun-Hay KoWing-Shing CheungKe-Wang LuoHui DaiChun-Kwok WongPing-Chung LeungQuan-Bin HanA polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25014571/pdf/?tool=EBI |
| spellingShingle | Xiao-Qiang Han Gar-Lee Yue Rui-Qi Yue Cai-Xia Dong Chung-Lap Chan Chun-Hay Ko Wing-Shing Cheung Ke-Wang Luo Hui Dai Chun-Kwok Wong Ping-Chung Leung Quan-Bin Han Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. PLoS ONE |
| title | Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_full | Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_fullStr | Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_full_unstemmed | Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_short | Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense. |
| title_sort | structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of ganoderma sinense |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25014571/pdf/?tool=EBI |
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