Primary Mammary Organoid Model of Lactation and Involution
Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00068/full |
| _version_ | 1819266360384946176 |
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| author | Jakub Sumbal Jakub Sumbal Aurelie Chiche Elsa Charifou Zuzana Koledova Han Li |
| author_facet | Jakub Sumbal Jakub Sumbal Aurelie Chiche Elsa Charifou Zuzana Koledova Han Li |
| author_sort | Jakub Sumbal |
| collection | DOAJ |
| description | Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer. |
| first_indexed | 2024-12-23T21:00:02Z |
| format | Article |
| id | doaj.art-90c79e24c72c42fbaebdd0cd1fa503ae |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-23T21:00:02Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-90c79e24c72c42fbaebdd0cd1fa503ae2022-12-21T17:31:24ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-03-01810.3389/fcell.2020.00068509220Primary Mammary Organoid Model of Lactation and InvolutionJakub Sumbal0Jakub Sumbal1Aurelie Chiche2Elsa Charifou3Zuzana Koledova4Han Li5Department of Developmental and Stem Cell Biology, Cellular Plasticity and Disease Modelling, CNRS UMR 3738, Institut Pasteur, Paris, FranceDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Developmental and Stem Cell Biology, Cellular Plasticity and Disease Modelling, CNRS UMR 3738, Institut Pasteur, Paris, FranceDepartment of Developmental and Stem Cell Biology, Cellular Plasticity and Disease Modelling, CNRS UMR 3738, Institut Pasteur, Paris, FranceDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Developmental and Stem Cell Biology, Cellular Plasticity and Disease Modelling, CNRS UMR 3738, Institut Pasteur, Paris, FranceMammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer.https://www.frontiersin.org/article/10.3389/fcell.2020.00068/full3D culturefibroblast growth factor 2involutionlactationmammary glandmilk production |
| spellingShingle | Jakub Sumbal Jakub Sumbal Aurelie Chiche Elsa Charifou Zuzana Koledova Han Li Primary Mammary Organoid Model of Lactation and Involution Frontiers in Cell and Developmental Biology 3D culture fibroblast growth factor 2 involution lactation mammary gland milk production |
| title | Primary Mammary Organoid Model of Lactation and Involution |
| title_full | Primary Mammary Organoid Model of Lactation and Involution |
| title_fullStr | Primary Mammary Organoid Model of Lactation and Involution |
| title_full_unstemmed | Primary Mammary Organoid Model of Lactation and Involution |
| title_short | Primary Mammary Organoid Model of Lactation and Involution |
| title_sort | primary mammary organoid model of lactation and involution |
| topic | 3D culture fibroblast growth factor 2 involution lactation mammary gland milk production |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00068/full |
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