Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections
In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. <i>Ralstonia</i> <i>solanacearum</i> and <i>Fusarium oxysporum f. sp. lycopersici</i> a...
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/10/2123 |
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| author | Nandhitha Venkatesh Max J. Koss Claudio Greco Grant Nickles Philipp Wiemann Nancy P. Keller |
| author_facet | Nandhitha Venkatesh Max J. Koss Claudio Greco Grant Nickles Philipp Wiemann Nancy P. Keller |
| author_sort | Nandhitha Venkatesh |
| collection | DOAJ |
| description | In order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. <i>Ralstonia</i> <i>solanacearum</i> and <i>Fusarium oxysporum f. sp. lycopersici</i> are vascular wilt pathogens that can result in heavy yield losses in susceptible hosts such as tomato. Although both pathogens occupy the xylem, the costs of mixed infections on wilt disease are unknown. Here, we characterize the consequences of co-infection with <i>R. solanacearum</i> and <i>F. oxysporum</i> using tomato as the model host. Our results demonstrate that bacterial wilt severity is reduced in co-infections, that bikaverin synthesis by <i>Fusarium </i>contributes to bacterial wilt reduction, and that the arrival time of each microbe at the infection court is important in driving the severity of wilt disease. Further, analysis of the co-infection root secretome identified previously uncharacterized secreted metabolites that reduce <i>R. solanacearum </i>growth in vitro and provide protection to tomato seedlings against bacterial wilt disease. Taken together, these results highlight the need to understand the consequences of mixed infections in plant disease. |
| first_indexed | 2024-03-10T06:22:11Z |
| format | Article |
| id | doaj.art-90c79e5303d646cba2b7bbff62fbef1f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T06:22:11Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-90c79e5303d646cba2b7bbff62fbef1f2023-11-22T19:14:40ZengMDPI AGMicroorganisms2076-26072021-10-01910212310.3390/microorganisms9102123Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-InfectionsNandhitha Venkatesh0Max J. Koss1Claudio Greco2Grant Nickles3Philipp Wiemann4Nancy P. Keller5Department of Plant Pathology, University of Wisconsin—Madison, Madison, WI 53706, USADepartment of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USADepartment of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USADepartment of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USADepartment of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USADepartment of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USAIn order to gain a comprehensive understanding of plant disease in natural and agricultural ecosystems, it is essential to examine plant disease in multi-pathogen–host systems. <i>Ralstonia</i> <i>solanacearum</i> and <i>Fusarium oxysporum f. sp. lycopersici</i> are vascular wilt pathogens that can result in heavy yield losses in susceptible hosts such as tomato. Although both pathogens occupy the xylem, the costs of mixed infections on wilt disease are unknown. Here, we characterize the consequences of co-infection with <i>R. solanacearum</i> and <i>F. oxysporum</i> using tomato as the model host. Our results demonstrate that bacterial wilt severity is reduced in co-infections, that bikaverin synthesis by <i>Fusarium </i>contributes to bacterial wilt reduction, and that the arrival time of each microbe at the infection court is important in driving the severity of wilt disease. Further, analysis of the co-infection root secretome identified previously uncharacterized secreted metabolites that reduce <i>R. solanacearum </i>growth in vitro and provide protection to tomato seedlings against bacterial wilt disease. Taken together, these results highlight the need to understand the consequences of mixed infections in plant disease.https://www.mdpi.com/2076-2607/9/10/2123secondary metabolitesplant–microbe interactionscoinfectionwilt diseasebacterial–fungal interactions<i>Fusarium oxysporum</i> |
| spellingShingle | Nandhitha Venkatesh Max J. Koss Claudio Greco Grant Nickles Philipp Wiemann Nancy P. Keller Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections Microorganisms secondary metabolites plant–microbe interactions coinfection wilt disease bacterial–fungal interactions <i>Fusarium oxysporum</i> |
| title | Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections |
| title_full | Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections |
| title_fullStr | Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections |
| title_full_unstemmed | Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections |
| title_short | Secreted Secondary Metabolites Reduce Bacterial Wilt Severity of Tomato in Bacterial–Fungal Co-Infections |
| title_sort | secreted secondary metabolites reduce bacterial wilt severity of tomato in bacterial fungal co infections |
| topic | secondary metabolites plant–microbe interactions coinfection wilt disease bacterial–fungal interactions <i>Fusarium oxysporum</i> |
| url | https://www.mdpi.com/2076-2607/9/10/2123 |
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