Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal
Wild birds may be healthy carriers, and therefore, may be involved in the dissemination of clinically relevant antimicrobial-resistant bacteria, such as extended-spectrum β-lactamases (ESBL) and carbapenemase-producing <i>Enterobacteriaceae</i>. This study evaluated whether urban pigeons...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1368 |
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| author | Samanta Freire Teresa Grilo Laurent Poirel Marta Aires-de-Sousa |
| author_facet | Samanta Freire Teresa Grilo Laurent Poirel Marta Aires-de-Sousa |
| author_sort | Samanta Freire |
| collection | DOAJ |
| description | Wild birds may be healthy carriers, and therefore, may be involved in the dissemination of clinically relevant antimicrobial-resistant bacteria, such as extended-spectrum β-lactamases (ESBL) and carbapenemase-producing <i>Enterobacteriaceae</i>. This study evaluated whether urban pigeons living in five spots in Lisbon, Portugal, may be colonized and, therefore, constitute potential spreaders of multidrug-resistant bacteria. A total of 100 pigeon fecal samples were collected in different urban areas for the detection of ESBL- or carbapenemase-producing <i>Enterobacteriaceae</i>. All β-lactamase-producing isolates were tested for antimicrobial susceptibility and their genetic backgrounds were characterized by multilocus sequence typing. Of the 100 fecal samples collected, nine ESBL-producing <i>Escherichia coli</i> (9%) were identified. Three isolates carried the <i>bla</i><sub>CTX-M-15</sub> gene, three isolates harbored the <i>bla</i><sub>CTX-M-27</sub> and three isolates carried the <i>bla</i><sub>SHV-12</sub> gene. Genotyping of the nine ESBL-producing <i>E. coli</i> strains revealed seven different sequence types (STs) including ST10, ST131, ST154, ST206, ST1488 (SLV ST10), ST2858 and ST3576, most of which have been already described in humans, animals or in the environment. Urban pigeons constitute a potential source of ESBL genes and may be a transmission vehicle of multidrug-resistant bacteria in the environment. |
| first_indexed | 2024-03-09T20:50:46Z |
| format | Article |
| id | doaj.art-90c82a3e660c48db8d6d9adee2bc373e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:50:46Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-90c82a3e660c48db8d6d9adee2bc373e2023-11-23T22:35:16ZengMDPI AGAntibiotics2079-63822022-10-011110136810.3390/antibiotics11101368Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, PortugalSamanta Freire0Teresa Grilo1Laurent Poirel2Marta Aires-de-Sousa3Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalLaboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalMedical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandLaboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalWild birds may be healthy carriers, and therefore, may be involved in the dissemination of clinically relevant antimicrobial-resistant bacteria, such as extended-spectrum β-lactamases (ESBL) and carbapenemase-producing <i>Enterobacteriaceae</i>. This study evaluated whether urban pigeons living in five spots in Lisbon, Portugal, may be colonized and, therefore, constitute potential spreaders of multidrug-resistant bacteria. A total of 100 pigeon fecal samples were collected in different urban areas for the detection of ESBL- or carbapenemase-producing <i>Enterobacteriaceae</i>. All β-lactamase-producing isolates were tested for antimicrobial susceptibility and their genetic backgrounds were characterized by multilocus sequence typing. Of the 100 fecal samples collected, nine ESBL-producing <i>Escherichia coli</i> (9%) were identified. Three isolates carried the <i>bla</i><sub>CTX-M-15</sub> gene, three isolates harbored the <i>bla</i><sub>CTX-M-27</sub> and three isolates carried the <i>bla</i><sub>SHV-12</sub> gene. Genotyping of the nine ESBL-producing <i>E. coli</i> strains revealed seven different sequence types (STs) including ST10, ST131, ST154, ST206, ST1488 (SLV ST10), ST2858 and ST3576, most of which have been already described in humans, animals or in the environment. Urban pigeons constitute a potential source of ESBL genes and may be a transmission vehicle of multidrug-resistant bacteria in the environment.https://www.mdpi.com/2079-6382/11/10/1368pigeonsESBL<i>Enterobacteriaceae</i><i>Escherichia coli</i>Portugal |
| spellingShingle | Samanta Freire Teresa Grilo Laurent Poirel Marta Aires-de-Sousa Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal Antibiotics pigeons ESBL <i>Enterobacteriaceae</i> <i>Escherichia coli</i> Portugal |
| title | Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal |
| title_full | Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal |
| title_fullStr | Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal |
| title_full_unstemmed | Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal |
| title_short | Urban Pigeons (<i>Columba livia</i>) as a Source of Broad-Spectrum β-Lactamase-Producing <i>Escherichia coli</i> in Lisbon, Portugal |
| title_sort | urban pigeons i columba livia i as a source of broad spectrum β lactamase producing i escherichia coli i in lisbon portugal |
| topic | pigeons ESBL <i>Enterobacteriaceae</i> <i>Escherichia coli</i> Portugal |
| url | https://www.mdpi.com/2079-6382/11/10/1368 |
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