Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles
<p>Abstract</p> <p>Background</p> <p>Previous studies reported divergent results on nutraceutical actions and free radical scavenging capability of ginseng extracts. Variations in ginsenoside profile of ginseng due to different soil and cultivating season may contribute...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2012-05-01
|
| Series: | Journal of the International Society of Sports Nutrition |
| Subjects: | |
| Online Access: | http://www.jissn.com/content/9/1/23 |
| _version_ | 1811227356964061184 |
|---|---|
| author | Yu Szu-Hsien Huang Hui-Yu Korivi Mallikarjuna Hsu Ming-Fen Huang Chih-Yang Hou Chien-Wen Chen Chung-Yu Kao Chung-Lan Lee Ru-Ping Lee Shin-Da Kuo Chia-Hua |
| author_facet | Yu Szu-Hsien Huang Hui-Yu Korivi Mallikarjuna Hsu Ming-Fen Huang Chih-Yang Hou Chien-Wen Chen Chung-Yu Kao Chung-Lan Lee Ru-Ping Lee Shin-Da Kuo Chia-Hua |
| author_sort | Yu Szu-Hsien |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Previous studies reported divergent results on nutraceutical actions and free radical scavenging capability of ginseng extracts. Variations in ginsenoside profile of ginseng due to different soil and cultivating season may contribute to the inconsistency. To circumvent this drawback, we assessed the effect of major ginsenoside-Rg1 (Rg1) on skeletal muscle antioxidant defense system against exhaustive exercise-induced oxidative stress.</p> <p>Methods</p> <p>Forty weight-matched rats were evenly divided into control (N = 20) and Rg1 (N = 20) groups. Rg1 was orally administered at the dose of 0.1 mg/kg bodyweight per day for 10-week. After this long-term Rg1 administration, ten rats from each group performed an exhaustive swimming, and remaining rats considered as non-exercise control. Tibialis anterior (TA) muscles were surgically collected immediately after exercise along with non-exercise rats.</p> <p>Results</p> <p>Exhaustive exercise significantly (p<0.05) increased the lipid peroxidation of control group, as evidenced by elevated malondialdehyde (MDA) levels. The increased oxidative stress after exercise was also confirmed by decreased reduced glutathione to oxidized glutathione ratio (GSH/GSSG ratio) in control rats. However, these changes were completely eliminated in Rg1 group. Catalase (CAT) and glutathione peroxidase (GPx) activities were significantly (p<0.05) increased by Rg1 in non-exercise rats, while no significant change after exercise. Nevertheless, glutathione reductase (GR) and glutathione S-transferase (GST) activities were significantly increased after exercise in Rg1 group.</p> <p>Conclusions</p> <p>This study provide compelling evidences that Rg1 supplementation can strengthen antioxidant defense system in skeletal muscle and completely attenuate the membrane lipid peroxidation induced by exhaustive exercise. Our findings suggest that Rg1 can use as a nutraceutical supplement to buffer the exhaustive exercise-induced oxidative stress.</p> |
| first_indexed | 2024-04-12T09:41:08Z |
| format | Article |
| id | doaj.art-90c9a71534f34c9b98286bb504d1d66d |
| institution | Directory Open Access Journal |
| issn | 1550-2783 |
| language | English |
| last_indexed | 2024-04-12T09:41:08Z |
| publishDate | 2012-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of the International Society of Sports Nutrition |
| spelling | doaj.art-90c9a71534f34c9b98286bb504d1d66d2022-12-22T03:38:04ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832012-05-01912310.1186/1550-2783-9-23Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal musclesYu Szu-HsienHuang Hui-YuKorivi MallikarjunaHsu Ming-FenHuang Chih-YangHou Chien-WenChen Chung-YuKao Chung-LanLee Ru-PingLee Shin-DaKuo Chia-Hua<p>Abstract</p> <p>Background</p> <p>Previous studies reported divergent results on nutraceutical actions and free radical scavenging capability of ginseng extracts. Variations in ginsenoside profile of ginseng due to different soil and cultivating season may contribute to the inconsistency. To circumvent this drawback, we assessed the effect of major ginsenoside-Rg1 (Rg1) on skeletal muscle antioxidant defense system against exhaustive exercise-induced oxidative stress.</p> <p>Methods</p> <p>Forty weight-matched rats were evenly divided into control (N = 20) and Rg1 (N = 20) groups. Rg1 was orally administered at the dose of 0.1 mg/kg bodyweight per day for 10-week. After this long-term Rg1 administration, ten rats from each group performed an exhaustive swimming, and remaining rats considered as non-exercise control. Tibialis anterior (TA) muscles were surgically collected immediately after exercise along with non-exercise rats.</p> <p>Results</p> <p>Exhaustive exercise significantly (p<0.05) increased the lipid peroxidation of control group, as evidenced by elevated malondialdehyde (MDA) levels. The increased oxidative stress after exercise was also confirmed by decreased reduced glutathione to oxidized glutathione ratio (GSH/GSSG ratio) in control rats. However, these changes were completely eliminated in Rg1 group. Catalase (CAT) and glutathione peroxidase (GPx) activities were significantly (p<0.05) increased by Rg1 in non-exercise rats, while no significant change after exercise. Nevertheless, glutathione reductase (GR) and glutathione S-transferase (GST) activities were significantly increased after exercise in Rg1 group.</p> <p>Conclusions</p> <p>This study provide compelling evidences that Rg1 supplementation can strengthen antioxidant defense system in skeletal muscle and completely attenuate the membrane lipid peroxidation induced by exhaustive exercise. Our findings suggest that Rg1 can use as a nutraceutical supplement to buffer the exhaustive exercise-induced oxidative stress.</p>http://www.jissn.com/content/9/1/23GinsengGinsenosideAntioxidant statusLipid peroxidationSwimmingMDAProtein carbonylOxidative damageFree radical attackSports nutritionSarcolemma |
| spellingShingle | Yu Szu-Hsien Huang Hui-Yu Korivi Mallikarjuna Hsu Ming-Fen Huang Chih-Yang Hou Chien-Wen Chen Chung-Yu Kao Chung-Lan Lee Ru-Ping Lee Shin-Da Kuo Chia-Hua Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles Journal of the International Society of Sports Nutrition Ginseng Ginsenoside Antioxidant status Lipid peroxidation Swimming MDA Protein carbonyl Oxidative damage Free radical attack Sports nutrition Sarcolemma |
| title | Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles |
| title_full | Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles |
| title_fullStr | Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles |
| title_full_unstemmed | Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles |
| title_short | Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles |
| title_sort | oral rg1 supplementation strengthens antioxidant defense system against exercise induced oxidative stress in rat skeletal muscles |
| topic | Ginseng Ginsenoside Antioxidant status Lipid peroxidation Swimming MDA Protein carbonyl Oxidative damage Free radical attack Sports nutrition Sarcolemma |
| url | http://www.jissn.com/content/9/1/23 |
| work_keys_str_mv | AT yuszuhsien oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT huanghuiyu oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT korivimallikarjuna oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT hsumingfen oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT huangchihyang oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT houchienwen oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT chenchungyu oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT kaochunglan oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT leeruping oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT leeshinda oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles AT kuochiahua oralrg1supplementationstrengthensantioxidantdefensesystemagainstexerciseinducedoxidativestressinratskeletalmuscles |