Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr.
Soybean transcription factor GmNAC plays important roles in plant resistance to environmental stresses. In this study, <i>GmNAC3</i> was cloned in the drought tolerant soybean variety “Jiyu47”, with the molecular properties of GmNAC3 characterized to establish its candidacy as a NAC tran...
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2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12378 |
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| author | Zhanyu Chen Xiaoqin Yang Minghao Tang Yujue Wang Qian Zhang Huiying Li Ying Zhou Fengjie Sun Xiyan Cui |
| author_facet | Zhanyu Chen Xiaoqin Yang Minghao Tang Yujue Wang Qian Zhang Huiying Li Ying Zhou Fengjie Sun Xiyan Cui |
| author_sort | Zhanyu Chen |
| collection | DOAJ |
| description | Soybean transcription factor GmNAC plays important roles in plant resistance to environmental stresses. In this study, <i>GmNAC3</i> was cloned in the drought tolerant soybean variety “Jiyu47”, with the molecular properties of GmNAC3 characterized to establish its candidacy as a NAC transcription factor. The yeast self-activation experiments revealed the transcriptional activation activity of GmNAC3, which was localized in the nucleus by the subcellular localization analysis. The highest expression of <i>GmNAC3</i> was detected in roots in the podding stage of soybean, and in roots of soybean seedlings treated with 20% PEG6000 for 12 h, which was 16 times higher compared with the control. In the transgenic soybean hairy roots obtained by the <i>Agrobacterium</i>-mediated method treated with 20% PEG6000 for 12 h, the activities of superoxide dismutase, peroxidase, and catalase and the content of proline were increased, the malondialdehyde content was decreased, and the expressions of stress resistance-related genes (i.e., <i>APX2</i>, <i>LEA14</i>, <i>6PGDH</i>, and <i>P5CS</i>) were up-regulated. These expression patterns were confirmed by transgenic <i>Arabidopsis thaliana</i> with the overexpression of <i>GmNAC3</i>. This study provided strong scientific evidence to support further investigation of the regulatory function of <i>GmNAC3</i> in plant drought resistance and the molecular mechanisms regulating the plant response to environmental stresses. |
| first_indexed | 2024-03-09T12:14:59Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:14:59Z |
| publishDate | 2022-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-90cb79bb8e55422faae66d714bd680012023-11-30T22:47:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201237810.3390/ijms232012378Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr.Zhanyu Chen0Xiaoqin Yang1Minghao Tang2Yujue Wang3Qian Zhang4Huiying Li5Ying Zhou6Fengjie Sun7Xiyan Cui8College of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaDepartment of Biological Sciences, School of Science and Technology, Georgia Gwinnett College, Lawrenceville, GA 30043, USACollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaSoybean transcription factor GmNAC plays important roles in plant resistance to environmental stresses. In this study, <i>GmNAC3</i> was cloned in the drought tolerant soybean variety “Jiyu47”, with the molecular properties of GmNAC3 characterized to establish its candidacy as a NAC transcription factor. The yeast self-activation experiments revealed the transcriptional activation activity of GmNAC3, which was localized in the nucleus by the subcellular localization analysis. The highest expression of <i>GmNAC3</i> was detected in roots in the podding stage of soybean, and in roots of soybean seedlings treated with 20% PEG6000 for 12 h, which was 16 times higher compared with the control. In the transgenic soybean hairy roots obtained by the <i>Agrobacterium</i>-mediated method treated with 20% PEG6000 for 12 h, the activities of superoxide dismutase, peroxidase, and catalase and the content of proline were increased, the malondialdehyde content was decreased, and the expressions of stress resistance-related genes (i.e., <i>APX2</i>, <i>LEA14</i>, <i>6PGDH</i>, and <i>P5CS</i>) were up-regulated. These expression patterns were confirmed by transgenic <i>Arabidopsis thaliana</i> with the overexpression of <i>GmNAC3</i>. This study provided strong scientific evidence to support further investigation of the regulatory function of <i>GmNAC3</i> in plant drought resistance and the molecular mechanisms regulating the plant response to environmental stresses.https://www.mdpi.com/1422-0067/23/20/12378<i>Glycine max</i>GmNAC3 transcription factordrought resistance<i>Arabidopsis</i>PEG6000superoxide dismutase |
| spellingShingle | Zhanyu Chen Xiaoqin Yang Minghao Tang Yujue Wang Qian Zhang Huiying Li Ying Zhou Fengjie Sun Xiyan Cui Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. International Journal of Molecular Sciences <i>Glycine max</i> GmNAC3 transcription factor drought resistance <i>Arabidopsis</i> PEG6000 superoxide dismutase |
| title | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. |
| title_full | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. |
| title_fullStr | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. |
| title_full_unstemmed | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. |
| title_short | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in <i>Glycine max</i> (L.) Merr. |
| title_sort | molecular characterization and drought resistance of gmnac3 transcription factor in i glycine max i l merr |
| topic | <i>Glycine max</i> GmNAC3 transcription factor drought resistance <i>Arabidopsis</i> PEG6000 superoxide dismutase |
| url | https://www.mdpi.com/1422-0067/23/20/12378 |
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