Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae)
Premise of the study: Microsatellite primers were developed in the endangered tree species <i>Taxus wallichiana</i> from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-03-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1200281 |
| _version_ | 1818359931795931136 |
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| author | Jyoti Prasad Gajurel Carolina Cornejo Silke Werth Krishna Kumar Shrestha Christoph Scheidegger |
| author_facet | Jyoti Prasad Gajurel Carolina Cornejo Silke Werth Krishna Kumar Shrestha Christoph Scheidegger |
| author_sort | Jyoti Prasad Gajurel |
| collection | DOAJ |
| description | Premise of the study: Microsatellite primers were developed in the endangered tree species <i>Taxus wallichiana</i> from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal.
Methods and Results: We developed 10 highly polymorphic microsatellite markers from 454 DNA sequencing. Characterization of the new microsatellite loci was done in 99 individuals collected from three valleys with different climatic regimes. The number of alleles per locus varied from four to 12. Observed heterozygosity of populations, averaged across loci, ranged from 0.30 to 0.59.
Conclusions: The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis. |
| first_indexed | 2024-12-13T20:52:44Z |
| format | Article |
| id | doaj.art-90cbbcc41c994dccbd21927ca2fbee90 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-13T20:52:44Z |
| publishDate | 2013-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-90cbbcc41c994dccbd21927ca2fbee902022-12-21T23:31:51ZengWileyApplications in Plant Sciences2168-04502013-03-0113120028110.3732/apps.1200281Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae)Jyoti Prasad Gajurel0Carolina Cornejo1Silke Werth2Krishna Kumar Shrestha3Christoph Scheidegger4Central Department of Botany, Tribhuvan University, Kathmandu, Nepal; Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, SwitzerlandSwiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, SwitzerlandSwiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, SwitzerlandCentral Department of Botany, Tribhuvan University, Kathmandu, NepalSwiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, SwitzerlandPremise of the study: Microsatellite primers were developed in the endangered tree species <i>Taxus wallichiana</i> from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal. Methods and Results: We developed 10 highly polymorphic microsatellite markers from 454 DNA sequencing. Characterization of the new microsatellite loci was done in 99 individuals collected from three valleys with different climatic regimes. The number of alleles per locus varied from four to 12. Observed heterozygosity of populations, averaged across loci, ranged from 0.30 to 0.59. Conclusions: The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis.http://www.bioone.org/doi/full/10.3732/apps.1200281454 pyrosequencingconservation geneticsHimalayan yewHimalayassimple sequence repeat marker |
| spellingShingle | Jyoti Prasad Gajurel Carolina Cornejo Silke Werth Krishna Kumar Shrestha Christoph Scheidegger Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) Applications in Plant Sciences 454 pyrosequencing conservation genetics Himalayan yew Himalayas simple sequence repeat marker |
| title | Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) |
| title_full | Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) |
| title_fullStr | Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) |
| title_full_unstemmed | Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) |
| title_short | Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae) |
| title_sort | development and characterization of microsatellite loci in the endangered species i taxus wallichiana i taxaceae |
| topic | 454 pyrosequencing conservation genetics Himalayan yew Himalayas simple sequence repeat marker |
| url | http://www.bioone.org/doi/full/10.3732/apps.1200281 |
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