Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering
As a gel scaffold for chondrocyte tissue engineering, agarose concentration plays a significant role in the relationship between porosity and nutrition. In this work, the effect of concentration and period cultured on Glycosaminoglycan (GAG) and mechanical properties have been studied. A bovine chon...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Vasyl Stefanyk Precarpathian National University
2021-12-01
|
| Series: | Фізика і хімія твердого тіла |
| Subjects: | |
| Online Access: | https://journals.pnu.edu.ua/index.php/pcss/article/view/5176 |
| _version_ | 1828134319889055744 |
|---|---|
| author | Akram Jawad |
| author_facet | Akram Jawad |
| author_sort | Akram Jawad |
| collection | DOAJ |
| description | As a gel scaffold for chondrocyte tissue engineering, agarose concentration plays a significant role in the relationship between porosity and nutrition. In this work, the effect of concentration and period cultured on Glycosaminoglycan (GAG) and mechanical properties have been studied. A bovine chondrocytes have been isolated and seeded in different agarose gel scoffed concentrations, about 4% and 6%, for different period cultured, 0 and 7 days. The MTS machine and Spectrophotometric with calibration curve method were used to measure mechanical properties, and GAG concentration of the prepared samples, respectively. The results of mechanical tests and GAG contents shown that there are a wide range of dispersion in the most of the samples, which attribute to different factors. For mechanical properties, these factors could be attributed to anisotropic of the produced chondrocyte with agarose scaffolds, insufficient cells' dispersion within the gel scaffold during seeding and cultured time, and some test procedure condition, such as EBSS hydration. While for GAG results, those factors could be the differences of the cell growth environment between in-vitro and in vivo media. |
| first_indexed | 2024-04-11T17:32:27Z |
| format | Article |
| id | doaj.art-90ce9ce3ee884267b13d332359a97b66 |
| institution | Directory Open Access Journal |
| issn | 1729-4428 2309-8589 |
| language | English |
| last_indexed | 2024-04-11T17:32:27Z |
| publishDate | 2021-12-01 |
| publisher | Vasyl Stefanyk Precarpathian National University |
| record_format | Article |
| series | Фізика і хімія твердого тіла |
| spelling | doaj.art-90ce9ce3ee884267b13d332359a97b662022-12-22T04:11:58ZengVasyl Stefanyk Precarpathian National UniversityФізика і хімія твердого тіла1729-44282309-85892021-12-0122476777410.15330/pcss.22.4.767-7745176Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue EngineeringAkram Jawad0University of Babylon; Queen Mary University of LondonAs a gel scaffold for chondrocyte tissue engineering, agarose concentration plays a significant role in the relationship between porosity and nutrition. In this work, the effect of concentration and period cultured on Glycosaminoglycan (GAG) and mechanical properties have been studied. A bovine chondrocytes have been isolated and seeded in different agarose gel scoffed concentrations, about 4% and 6%, for different period cultured, 0 and 7 days. The MTS machine and Spectrophotometric with calibration curve method were used to measure mechanical properties, and GAG concentration of the prepared samples, respectively. The results of mechanical tests and GAG contents shown that there are a wide range of dispersion in the most of the samples, which attribute to different factors. For mechanical properties, these factors could be attributed to anisotropic of the produced chondrocyte with agarose scaffolds, insufficient cells' dispersion within the gel scaffold during seeding and cultured time, and some test procedure condition, such as EBSS hydration. While for GAG results, those factors could be the differences of the cell growth environment between in-vitro and in vivo media.https://journals.pnu.edu.ua/index.php/pcss/article/view/5176chondrocytetissue engineeringagarosebiomaterialsglycosaminoglycan |
| spellingShingle | Akram Jawad Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering Фізика і хімія твердого тіла chondrocyte tissue engineering agarose biomaterials glycosaminoglycan |
| title | Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering |
| title_full | Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering |
| title_fullStr | Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering |
| title_full_unstemmed | Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering |
| title_short | Investigation of the Effect of Agarose Gel Concentration and Culture Period on Bio and Mechanical Properties of Chondrocyte Tissue Engineering |
| title_sort | investigation of the effect of agarose gel concentration and culture period on bio and mechanical properties of chondrocyte tissue engineering |
| topic | chondrocyte tissue engineering agarose biomaterials glycosaminoglycan |
| url | https://journals.pnu.edu.ua/index.php/pcss/article/view/5176 |
| work_keys_str_mv | AT akramjawad investigationoftheeffectofagarosegelconcentrationandcultureperiodonbioandmechanicalpropertiesofchondrocytetissueengineering |